Protective Mechanism of <italic style="font-style: italic">Cordyceps sinensis</italic> Treatment on Acute Kidney Injury-Induced Acute Lung Injury through AMPK/mTOR Signaling Pathway

WANG Ruo-lin; LIU Shu-hua; SHEN Si-heng; JIAN Lu-yong; YUAN Qi; GUO Hua-hui; HUANG Jia-sheng; CHEN Peng-hui; HUANG Ren-fa

doi:10.1007/s11655-023-3593-4

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Protective Mechanism of Cordyceps sinensis Treatment on Acute Kidney Injury-Induced Acute Lung Injury through AMPK/mTOR Signaling Pathway

Original Article | Updated：2023-09-26

- Protective Mechanism of Cordyceps sinensis Treatment on Acute Kidney Injury-Induced Acute Lung Injury through AMPK/mTOR Signaling Pathway
- Protective Mechanism of Cordyceps sinensis Treatment on Acute Kidney Injury-Induced Acute Lung Injury through AMPK/mTOR Signaling Pathway
- 中国结合医学杂志（英文版） 2023年29卷第10期页码：875-884
- Affiliations：
  
  1.Nephropathy Department, Shenzhen Hospital (Futian)of Guangzhou University of Chinese Medicine, Shenzhen, Guangdong Province (518034), China
  2.The Third Clinical Medical School, Guangzhou University of Chinese Medicine, Guangzhou (510006), China
- Author bio：
  
  Prof. HUANG Ren-fa, E-mail: huangrenfa1972@163.com
- Funds：
  
  the National Natural Science Foundation of China(81460682);the Natural Science Foundation of Guangdong Province(2020A1515010566);the Shenzhen Foundation of Science and Technology Research and Development(JCYJ20190809102413156)
- DOI：10.1007/s11655-023-3593-4
  中图分类号：
- 纸质出版日期：2023-10，
  
  网络出版日期：2023-02-27，
  
  录用日期：2022-10-09
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Protective Mechanism of Cordyceps sinensis Treatment on Acute Kidney Injury-Induced Acute Lung Injury through AMPK/mTOR Signaling Pathway[J]. 中国结合医学杂志（英文版）, 2023,29(10):875-884.

WANG Ruo-lin, LIU Shu-hua, SHEN Si-heng, et al. Protective Mechanism of Cordyceps sinensis Treatment on Acute Kidney Injury-Induced Acute Lung Injury through AMPK/mTOR Signaling Pathway[J]. Chinese Journal of Integrative Medicine, 2023,29(10):875-884.
Protective Mechanism of Cordyceps sinensis Treatment on Acute Kidney Injury-Induced Acute Lung Injury through AMPK/mTOR Signaling Pathway[J]. 中国结合医学杂志（英文版）, 2023,29(10):875-884. DOI： 10.1007/s11655-023-3593-4.

WANG Ruo-lin, LIU Shu-hua, SHEN Si-heng, et al. Protective Mechanism of Cordyceps sinensis Treatment on Acute Kidney Injury-Induced Acute Lung Injury through AMPK/mTOR Signaling Pathway[J]. Chinese Journal of Integrative Medicine, 2023,29(10):875-884. DOI： 10.1007/s11655-023-3593-4.

摘要

Abstract

Objective:

To investigate protective effect of

Cordyceps sinensis

(CS) through autophagy-associated adenosine monophosphate-activated protein kinase (AMPK)/mammalian target of rapamycin (mTOR) signaling pathway in acute kidney injury (AKI)-induced acute lung injury (ALI).

Methods:

Forty-eight male Sprague-Dawley rats were divided into 4 groups according to a random number table

including the normal saline (NS)-treated sham group (sham group)

NS-treated ischemia reperfusion injury (IRI) group (IRI group)

and low- (5 g/kg•d) and high-dose (10 g/kg•d) CS-treated IRI groups (CS1 and CS2 groups)

12 rats in each group. Nephrectomy of the right kidney was performed on the IRI rat model that was subjected to 60 min of left renal pedicle occlusion followed by 12

and 72 h of reperfusion. The wet-to-dry (W/D) ratio of lung

levels of serum creatinine (Scr)

blood urea nitrogen (BUN)

inflammatory cytokines such as interleukin-β and tumor necrosis factor-α

and biomarkers of oxidative stress such as superoxide dismutase

malonaldehyde (MDA) and myeloperoxidase (MPO)

were assayed. Histological examinations were conducted to determine damage of tissues in the kidney and lung. The protein expressions of light chain 3 Ⅱ/light chain 3 Ⅰ (LC3-Ⅱ/LC3-Ⅰ)

uncoordinated-51-like kinase 1 (ULK1)

P62

AMPK and mTOR were measured by Western blot and immunohistochemistry

respectively.

Results:

The renal IRI induced pulmonary injury following AKI

resulting in significant increases in W/D ratio of lung

and the levels of Scr

BUN

inflammatory cytokines

MDA and MPO (

0.01); all of these were reduced in the CS groups (

0.05 or

0.01). Compared with the IRI groups

the expression levels of P62 and mTOR were significantly lower (

0.05 or

0.01)

while those of LC3-Ⅱ/LC3-Ⅰ

ULK1

and AMPK were significantly higher in the CS2 group (

0.05 or

0.01).

Conclusion:

CS had a potential in treating lung injury following renal IRI through activation of the autophagy-related AMPK/mTOR signaling pathway in AKI-induced ALI.

关键词

Keywords

autophagyischemic reperfusion injuryacute kidney injuryacute lung injuryCordyceps sinensisChinese medicine

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Protective Mechanism of Cordyceps sinensis Treatment on Acute Kidney Injury-Induced Acute Lung Injury through AMPK/mTOR Signaling Pathway

Protective Mechanism of Cordyceps sinensis Treatment on Acute Kidney Injury-Induced Acute Lung Injury through AMPK/mTOR Signaling Pathway

DOI：10.1007/s11655-023-3593-4

摘要

Abstract

关键词

Keywords

references