Total Glucosides of Paeony Regulate Immune Imbalance Mediated by Dermal Mesenchymal Stem Cells in Psoriasis Mice

LEI Ming-jun; BAI Fan; ZHANG Qing-yun; YANG Qing-qing; TIAN Zan

doi:10.1007/s11655-023-3737-y

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Total Glucosides of Paeony Regulate Immune Imbalance Mediated by Dermal Mesenchymal Stem Cells in Psoriasis Mice

Original Article | Updated：2023-05-25

- Total Glucosides of Paeony Regulate Immune Imbalance Mediated by Dermal Mesenchymal Stem Cells in Psoriasis Mice
- Total Glucosides of Paeony Regulate Immune Imbalance Mediated by Dermal Mesenchymal Stem Cells in Psoriasis Mice
- 中国结合医学杂志（英文版） 2023年29卷第6期页码：517-525
- Affiliations：
  
  Department of Dermatology, Hebei Province Hospital of Chinese Medicine, Shijiazhuang (050011), China
- Author bio：
  
  Prof. TIAN Zan, E-mail: tianzan2021@163.com
- Funds：
  
  Hebei Provincial Administration of Traditional Chinese Medicine(2020016)
- DOI：10.1007/s11655-023-3737-y
  中图分类号：
- 纸质出版日期：2023-06，
  
  录用日期：2023-01-28
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Total Glucosides of Paeony Regulate Immune Imbalance Mediated by Dermal Mesenchymal Stem Cells in Psoriasis Mice[J]. 中国结合医学杂志（英文版）, 2023,29(6):517-525.

LEI Ming-jun, BAI Fan, ZHANG Qing-yun, et al. Total Glucosides of Paeony Regulate Immune Imbalance Mediated by Dermal Mesenchymal Stem Cells in Psoriasis Mice[J]. Chinese Journal of Integrative Medicine, 2023,29(6):517-525.
Total Glucosides of Paeony Regulate Immune Imbalance Mediated by Dermal Mesenchymal Stem Cells in Psoriasis Mice[J]. 中国结合医学杂志（英文版）, 2023,29(6):517-525. DOI： 10.1007/s11655-023-3737-y.

LEI Ming-jun, BAI Fan, ZHANG Qing-yun, et al. Total Glucosides of Paeony Regulate Immune Imbalance Mediated by Dermal Mesenchymal Stem Cells in Psoriasis Mice[J]. Chinese Journal of Integrative Medicine, 2023,29(6):517-525. DOI： 10.1007/s11655-023-3737-y.

摘要

Abstract

Objective:

To investigate the therapeutic effects of total glucosides of paeony (TGP) on psoriasis based on the immunomodulatory effect of dermal mesenchymal stem cells (DMSCs).

Methods:

A total of 30 male BALB/c mice were divided into 6 groups (

=5 in each) by a random number table method

including control

psoriasis model (model

5% imiquimod cream 42 mg/d)

low-

medium- and high-dose TGP (50

100

and 200 mg/kg

M-

and H-TGP

respectively)

and positive control group (2.5 mg/kg acitretin). After 14 days of continuous administration

the skin's histopathological changes

apoptosis

secretion of inflammatory cytokines

and proportion of regulatory T cells (Treg) and T helper cell 17 (Th17) were evaluated using hematoxylin-eosin (HE) staining

TdT-mediated dUTP nick end labeling staining

enzyme-linked immunosorbent assay

and flow cytometry

respectively. DMSCs were further isolated from the skin tissues of normal and psoriatic mice

and the cell morphology

phenotype

and cycle were observed. Furthermore

TGP was used to treat psoriatic DMSCs to analyze the effects on the DMSCs immune regulation.

Results:

TGP alleviated skin pathological injury

reduced epidermis layer thickness

inhibited apoptosis

and regulated the secretion of inflammatory cytokines and the proportion of Treg and Th17 in the skin tissues of psoriatic mice (

0.05 or

0.01). There was no significant difference in cell morphology and phenotype between control and psoriatic DMSCs (

0.05)

however

more psoriatic DMSCs remained in G

phase compared with the normal DMSCs (

0.01). TGP treatment of psoriatic DMSCs significantly increased cell viability

decreased apoptosis

relieved inflammatory response

and inhibited the expression of toll-like receptor 4 and P65 (

0.05 or

0.01).

Conclusion:

TGP may exert a good therapeutic effect on psoriasis by regulating the immune imbalance of DMSCs.

关键词

Keywords

total glucosides of paeonypsoriasisdermal mesenchymal stem cellsinflammatory responseimmune imbalance

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