Pien Tze Huang Inhibits Proliferation of Colorectal Cancer Cells through Suppressing PNO1 Expression and Activating p53/p21 Signaling Pathway

CAO Liu-jing; LIU Li-ya; CHEN You-qin; HAN Yu-ying; WEI Li-hui; YAO Meng-ying; FANG Yi; WU Mei-zhu; CHENG Ying; Thomas J. Sferra; LIU Hui-xin; LI Li; PENG Jun; SHEN A-ling

doi:10.1007/s11655-024-3709-5

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Pien Tze Huang Inhibits Proliferation of Colorectal Cancer Cells through Suppressing PNO1 Expression and Activating p53/p21 Signaling Pathway

Original Article | Updated：2024-07-03

- Pien Tze Huang Inhibits Proliferation of Colorectal Cancer Cells through Suppressing PNO1 Expression and Activating p53/p21 Signaling Pathway
- Pien Tze Huang Inhibits Proliferation of Colorectal Cancer Cells through Suppressing PNO1 Expression and Activating p53/p21 Signaling Pathway
- 中国结合医学杂志（英文版） 2024年30卷第6期页码：515-524
- Affiliations：
  
  1.Clinical Research Institute, the Second Affiliated Hospital &Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou (350122), China
  2.Fujian Key Laboratory of Integrative Medicine in Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou (350122), China
  3.Department of Pediatrics, Case Western Reserve University School of Medicine, UH Rainbow Babies and Children's Hospital, Cleveland, OH (44106), USA
  4.Innovation and Transformation Center, Fujian University of Traditional Chinese Medicine, Fuzhou(350122), China
  5.Department of Health Management, Fujian Provincial Hospital, Shengli Clinical College of Fujian Medical University, Fuzhou (350001), China
- Author bio：
  
  Prof. SHEN A-ling, E-mail: saling86@hotmail.com
- Funds：
  
  National Natural Science Foundation of China(81803882;82274188;82274148);Natural Science Foundation of Fujian Province(2020J06026)
- DOI：10.1007/s11655-024-3709-5
  中图分类号：
- 纸质出版日期：2024-06，
  
  网络出版日期：2024-01-13，
  
  录用日期：2023-06-02
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Pien Tze Huang Inhibits Proliferation of Colorectal Cancer Cells through Suppressing PNO1 Expression and Activating p53/p21 Signaling Pathway[J]. 中国结合医学杂志（英文版）, 2024,30(6):515-524.

CAO Liu-jing, LIU Li-ya, CHEN You-qin, et al. Pien Tze Huang Inhibits Proliferation of Colorectal Cancer Cells through Suppressing PNO1 Expression and Activating p53/p21 Signaling Pathway[J]. Chinese Journal of Integrative Medicine, 2024,30(6):515-524.
Pien Tze Huang Inhibits Proliferation of Colorectal Cancer Cells through Suppressing PNO1 Expression and Activating p53/p21 Signaling Pathway[J]. 中国结合医学杂志（英文版）, 2024,30(6):515-524. DOI： 10.1007/s11655-024-3709-5.

CAO Liu-jing, LIU Li-ya, CHEN You-qin, et al. Pien Tze Huang Inhibits Proliferation of Colorectal Cancer Cells through Suppressing PNO1 Expression and Activating p53/p21 Signaling Pathway[J]. Chinese Journal of Integrative Medicine, 2024,30(6):515-524. DOI： 10.1007/s11655-024-3709-5.

摘要

Abstract

Objective:

To explore the regulatory effect of Pien Tze Huang (PZH) on targeting partner of NOB1 (PNO1) and it's down-stream mediators in colorectal cancer (CRC) cells.

Methods:

Quantitative polymerase chain reaction was performed to determine mRNA levels of PNO1

TP53

and CDKN1A. Western blotting was performed to determine protein levels of PNO1

p53

and p21. HCT-8 cells were transduced with a lentivirus over-expressing PNO1. Colony formation assay was used to detect cell survival in PNO1 overexpression of HCT-8 cells after PZH treatment. Cell-cycle distribution

cell viability and cell apoptosis were performed to identify the effect of PNO1 overexpression on cell proliferation and apoptosis of HCT-8 cells after PZH treatment. Xenograft BALB/c nude mice bearing HCT116 cells transduced with sh-PNO1 or sh-Ctrl lentivirus were evaluated. Western blot assay was performed to detect PNO1

p53

p21 and PCNA expression in tumor sections. Terminal deoxynucleotidyl transferase dUTP nick end labling (TUNEL) assay was used to determine the apoptotic cells in tissues.

Results:

PZH treatment decreased cell viability

down-regulated PNO1 expression

and up-regulated p53 and p21 expressions in HCT-8 cells (

0.05). PNO1 overexpression attenuated the effects of PZH treatment

including the expression of p53 and p21

cell growth

cell viability

cell cycle arrest and cell apoptosis

in vitro

(

0.05). PNO1 knockdown eliminated the effects of PZH treatment on tumor growth

inhibiting cell proliferation inhibition and apoptosis induction

in vivo

(

0.05). Similarly

PNO1 knockdown attenuated the effects of PZH treatment on the downregulation of PNO1 and up regulation of p53 and p21

in vivo

(

0.05).

Conclusion:

The mechanism by which PZH induces its CRC anti-proliferative effect is at least in part by regulating the expression of PNO1 and its downstream targets p53 and p21.

关键词

Keywords

colorectal cancerPien Tze HuangChinese medicinepartner of NOB1proliferationp53/p21 pathway

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