Chrysophanol Induces Cell Death and Inhibits Invasiveness through Alteration of Calcium Levels in HepG2 Human Liver Cancer Cells<sup>*</sup>

CHEN Shu-chao; CHEN Qiao-wen; KO Chih-yuan

doi:10.1007/s11655-024-3817-2

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Chrysophanol Induces Cell Death and Inhibits Invasiveness through Alteration of Calcium Levels in HepG2 Human Liver Cancer Cells^*

Original Article | Updated：2025-04-22

- Chrysophanol Induces Cell Death and Inhibits Invasiveness through Alteration of Calcium Levels in HepG2 Human Liver Cancer Cells^*
- Chrysophanol Induces Cell Death and Inhibits Invasiveness through Alteration of Calcium Levels in HepG2 Human Liver Cancer Cells^*
- 中国结合医学杂志（英文版） 2025年31卷第5期页码：434-440
- Affiliations：
  
  1.Department of Respiratory and Critical Care Medicine, the Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian Province (362000), China
  2.Department of Clinical Nutrition, the Second Affiliated Hospital of Fujian Medical University, Quanzhou (362000), Fujian Province, China
  3.School of Public Health, Fujian Medical University, Fuzhou (350122), China
- Author bio：
  
  Dr. KO Chih-yuan, E-mail: yuanmomoko@gmail.com
- Funds：
  
  Academic Funding from the Second Affiliated Hospital of Fujian Medical University(BS201902);Quanzhou Science and Technology Project (the Malignant Tumor Clinical Medicine Research Center, Quanzhou City, Fujian Province, China)(2020N090S)
- DOI：10.1007/s11655-024-3817-2
  中图分类号：
- 录用日期：2024-04-30，
  
  网络出版日期：2024-08-05，
  
  纸质出版日期：2025-05
- Accepted：
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Chrysophanol Induces Cell Death and Inhibits Invasiveness through Alteration of Calcium Levels in HepG2 Human Liver Cancer Cells^*[J]. 中国结合医学杂志（英文版）, 2025,31(5):434-440.

CHEN Shu-chao, CHEN Qiao-wen, KO Chih-yuan. Chrysophanol Induces Cell Death and Inhibits Invasiveness through Alteration of Calcium Levels in HepG2 Human Liver Cancer Cells^*[J]. Chinese journal of integrative medicine, 2025, 31(5): 434-440.
Chrysophanol Induces Cell Death and Inhibits Invasiveness through Alteration of Calcium Levels in HepG2 Human Liver Cancer Cells^*[J]. 中国结合医学杂志（英文版）, 2025,31(5):434-440. DOI： 10.1007/s11655-024-3817-2.

CHEN Shu-chao, CHEN Qiao-wen, KO Chih-yuan. Chrysophanol Induces Cell Death and Inhibits Invasiveness through Alteration of Calcium Levels in HepG2 Human Liver Cancer Cells^*[J]. Chinese journal of integrative medicine, 2025, 31(5): 434-440. DOI： 10.1007/s11655-024-3817-2.

摘要

Abstract

Objective:

To investigate the effect of chrysophanol

a phytochemical derived from

Radix et Rhizoma Rhei

on HepG2 liver cancer cells.

Methods:

HepG2 cell line was treated with different concentrations chrysophanol (0–100 μmol/L) for 24 h. The cell counting kit 8 assay was employed to assess cell viability. Intracellular calcium levels were examined using Fluo-4 AM and Mag-fluo-4 AM staining

followed by flow cytometry analysis. Mitochondrial membrane potential was measured with JC-1 assay kit. Additionally

the expressions of key proteins such as p-JNK

Bax

cytochrome c (Cyt C)

cleaved caspase-3 (cCaspase-3)

and caspase-8 were analyzed by Western blot. The inhibitory effects of chrysophanol on the invasion of cells were determined using a Transwell assay. Analysis of invasiveness was conducted by wound healing assay.

Results:

Chrysophanol significantly reduced the proliferation of HepG2 liver cancer cells by affecting intracellular calcium distribution

diminishing mitochondrial membrane potential

and enhancing the expressions of p-JNK

Bax

Cyt C

cCaspase-3

and caspase-8 in the groups treated with 75 or 100 μmol/L chrysophanol compared to the control group (

0.05). Additionally

75 and 100 μmol/L chrysophanol exhibited inhibitory effects on cell migration and wound healing.

Conclusion:

Chrysophanol demonstrates potential against HepG2 liver cancer cells

suggesting its potential use as a therapeutic agent for liver cancer treatment.

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