Multidimensional Analgesia of Acupuncture by Increasing Expression of MD2 in Central Nervous System

LI Wan-rong; REN Lu-lu; ZHAO Tian-tian; DAI Dan-qing; GAO Xiao-fei; LIANG Hua-zheng; XIONG Li-ze

doi:10.1007/s11655-024-4106-9

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Multidimensional Analgesia of Acupuncture by Increasing Expression of MD2 in Central Nervous System

Acupuncture Research | 更新时间：2024-10-29

- Multidimensional Analgesia of Acupuncture by Increasing Expression of MD2 in Central Nervous System
- Multidimensional Analgesia of Acupuncture by Increasing Expression of MD2 in Central Nervous System
- 角色： First author , 第一作者 ,
  
  机构：
  Shanghai Key Laboratory of Anesthesiology and Brain Functional Modulation, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai (200434), China
  Clinical Research Center for Anesthesiology and Perioperative Medicine, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai (200434), China
  Translational Research Institute of Brain and Brain-Like Intelligence, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai (200434), China
  Department of Anesthesiology and Perioperative Medicine, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai (200434), China
  
  邮箱：
  
  暂无本作者相关信息
  LI Wan-rong
  1234 ，
  角色：
  
  机构：
  School of Rehabilitation, Southwest Jiaotong University, Chengdu (618099), China
  
  邮箱：
  
  暂无本作者相关信息
  REN Lu-lu
  5 ，
  角色：
  
  机构：
  Shanghai Key Laboratory of Anesthesiology and Brain Functional Modulation, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai (200434), China
  Clinical Research Center for Anesthesiology and Perioperative Medicine, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai (200434), China
  Translational Research Institute of Brain and Brain-Like Intelligence, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai (200434), China
  Department of Anesthesiology and Perioperative Medicine, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai (200434), China
  
  邮箱：
  
  暂无本作者相关信息
  ZHAO Tian-tian
  1234 ，
  角色：
  
  机构：
  Shanghai Key Laboratory of Anesthesiology and Brain Functional Modulation, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai (200434), China
  Clinical Research Center for Anesthesiology and Perioperative Medicine, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai (200434), China
  Translational Research Institute of Brain and Brain-Like Intelligence, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai (200434), China
  Department of Anesthesiology and Perioperative Medicine, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai (200434), China
  
  邮箱：
  
  暂无本作者相关信息
  DAI Dan-qing
  1234 ，
  角色：
  
  机构：
  Shanghai Key Laboratory of Anesthesiology and Brain Functional Modulation, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai (200434), China
  Clinical Research Center for Anesthesiology and Perioperative Medicine, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai (200434), China
  Translational Research Institute of Brain and Brain-Like Intelligence, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai (200434), China
  Department of Anesthesiology and Perioperative Medicine, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai (200434), China
  
  邮箱：
  
  暂无本作者相关信息
  GAO Xiao-fei
  1234 ，
  角色：
  
  机构：
  Shanghai Key Laboratory of Anesthesiology and Brain Functional Modulation, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai (200434), China
  Clinical Research Center for Anesthesiology and Perioperative Medicine, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai (200434), China
  Translational Research Institute of Brain and Brain-Like Intelligence, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai (200434), China
  Department of Anesthesiology and Perioperative Medicine, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai (200434), China
  Monash Suzhou Research Institute, Suzhou, Jiangsu Province (215125), China
  
  邮箱：
  
  暂无本作者相关信息
  LIANG Hua-zheng
  12346 ，
  角色： Corresponding author , 通讯作者 ,
  
  机构：
  Shanghai Key Laboratory of Anesthesiology and Brain Functional Modulation, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai (200434), China
  Clinical Research Center for Anesthesiology and Perioperative Medicine, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai (200434), China
  Translational Research Institute of Brain and Brain-Like Intelligence, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai (200434), China
  Department of Anesthesiology and Perioperative Medicine, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai (200434), China
  
  邮箱： lizexiong@tongji.edu.cn
  
  简介： Prof. XIONG Li-ze, E-mail: lizexiong@tongji.edu.cn
  
  暂无本作者相关信息
  XIONG Li-ze
  1234 ，
- 中国结合医学杂志（英文版） 2024年30卷第11期页码：1035-1044
- 作者机构：
  
  1.Shanghai Key Laboratory of Anesthesiology and Brain Functional Modulation, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai (200434), China
  2.Clinical Research Center for Anesthesiology and Perioperative Medicine, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai (200434), China
  3.Translational Research Institute of Brain and Brain-Like Intelligence, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai (200434), China
  4.Department of Anesthesiology and Perioperative Medicine, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai (200434), China
  5.School of Rehabilitation, Southwest Jiaotong University, Chengdu (618099), China
  6.Monash Suzhou Research Institute, Suzhou, Jiangsu Province (215125), China
- 作者简介：
  
  Prof. XIONG Li-ze, E-mail: lizexiong@tongji.edu.cn
- 基金信息：
  
  the National Natural Science Foundation of China(82130121;81974535);the Second Round of the Three-Year Action Plan for "Strengthening and Promoting Traditional Chinese Medicine" of Hongkou District, Shanghai(HKGYQYXM-2022-06)
- DOI：10.1007/s11655-024-4106-9
  中图分类号：
- 纸质出版日期：2024-11，
  
  网络出版日期：2024-07-29，
  
  录用日期：2023-11-15
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引用本文
Multidimensional Analgesia of Acupuncture by Increasing Expression of MD2 in Central Nervous System[J]. 中国结合医学杂志（英文版）, 2024,30(11):1035-1044.

LI Wan-rong, REN Lu-lu, ZHAO Tian-tian, et al. Multidimensional Analgesia of Acupuncture by Increasing Expression of MD2 in Central Nervous System[J]. Chinese Journal of Integrative Medicine, 2024,30(11):1035-1044.
Multidimensional Analgesia of Acupuncture by Increasing Expression of MD2 in Central Nervous System[J]. 中国结合医学杂志（英文版）, 2024,30(11):1035-1044. DOI： 10.1007/s11655-024-4106-9.

LI Wan-rong, REN Lu-lu, ZHAO Tian-tian, et al. Multidimensional Analgesia of Acupuncture by Increasing Expression of MD2 in Central Nervous System[J]. Chinese Journal of Integrative Medicine, 2024,30(11):1035-1044. DOI： 10.1007/s11655-024-4106-9.

Multidimensional Analgesia of Acupuncture by Increasing Expression of MD2 in Central Nervous System

LI Wan-rong ; REN Lu-lu ; ZHAO Tian-tian ; DAI Dan-qing ; GAO Xiao-fei ; LIANG Hua-zheng ; XIONG Li-ze ;

Abstract

Objective:

To investigate changes of myeloid differentiation factor 2 (MD2) in inflammation-induced pain and acupuncture-mediated analgesia.

译

Methods:

Mice were randomly divided into three groups by a random number table method: saline group (n=16), complete Freund's adjuvant (CFA) group (n=24) and CFA+electroacupuncture (EA) group (n=26). Inflammation-induced pain was modelled by injecting CFA to the plantar surface of the hind paw of mice and EA was applied to bilateral Zusanli (ST 36) to alleviate pain. Only mice in the CFA+EA group received EA treatment (30 min/d for 2 weeks) 24 h after modelling. Mice in the saline and CFA groups received sham EA. von-Frey test and Hargreaves test were used to assess the pain threshold. Brain and spinal tissues were collected for immunofluorescence staining or Western blotting to quantify changes of MD2 expression.

译

Results:

CFA successfully induced plantar pain and EA significantly alleviated pain 3 days after modelling (P<0.01). Compared with the CFA group, the number of MD2⁺/c-fos⁺ neurons was significantly increased in the dorsal horn of the spinal cord 7 and 14 days after EA, especially in laminae Ⅰ–Ⅱ_o (P<0.01). The proportion of double positive cells to the number of c-fos positive cells and the mean fluorescence intensity of MD2 neurons were also significantly increased in laminae Ⅰ–Ⅱ_o (P<0.01). Western blotting showed that the level of MD2 was significantly decreased by EA only in the hippocampus on day 7 and 14 (both P<0.01) and no significant changes were observed in the cortex, thalamus, cerebellum, or the brainstem (P>0.05). Fluorescence staining showed significant decrease in the level of MD2 in periagueductal gray (PAG) and locus coeruleus (LC) after CFA injection on day 7 (P<0.01 for PAG, P<0.05 for LC) and EA significantly reversed this decrease (P<0.01 for PAG, P<0.05 for LC).

译

Conclusion:

The unique changes of MD2 suggest that EA may exert the analgesic effect through modulating neuronal activities of the superficial laminae of the spinal cord and certain regions of the brain.

译

Keywords

electroacupuncture; myeloid differentiation factor 2; inflammation-induced pain; dorsal horn; periaqueductal gray; locus coeruleus

译

摘要

Abstract

Objective:

To investigate changes of myeloid differentiation factor 2 (MD2) in inflammation-induced pain and acupuncture-mediated analgesia.

Methods:

Mice were randomly divided into three groups by a random number table method: saline group (

=16)

complete Freund's adjuvant (CFA) group (

=24) and CFA+electroacupuncture (EA) group (

=26). Inflammation-induced pain was modelled by injecting CFA to the plantar surface of the hind paw of mice and EA was applied to bilateral Zusanli (ST 36) to alleviate pain. Only mice in the CFA+EA group received EA treatment (30 min/d for 2 weeks) 24 h after modelling. Mice in the saline and CFA groups received sham EA. von-Frey test and Hargreaves test were used to assess the pain threshold. Brain and spinal tissues were collected for immunofluorescence staining or Western blotting to quantify changes of MD2 expression.

Results:

CFA successfully induced plantar pain and EA significantly alleviated pain 3 days after modelling (

0.01). Compared with the CFA group

the numb

er of MD2

/c-fos

neurons was significantly increased in the dorsal horn of the spinal cord 7 and 14 days after EA

especially in laminae Ⅰ–Ⅱ

(

0.01). The proportion of double positive cells to the number of c-fos positive cells and the mean fluorescence intensity of MD2 neurons were also significantly increased in laminae Ⅰ–Ⅱ

(

0.01). Western blotting showed that the level of MD2 was significantly decreased by EA only in the hippocampus on day 7 and 14 (both

0.01) and no significant changes were observed in the cortex

thalamus

cerebellum

or the brainstem (

0.05). Fluorescence staining showed significant decrease in the level of MD2 in periagueductal gray (PAG) and locus coeruleus (LC) after CFA injection on day 7 (

0.01 for PAG

0.05 for LC) and EA significantly reversed this decrease (

0.01 for PAG

0.05 for LC).

Conclusion:

The unique changes of MD2 suggest that EA may exert the analgesic effect through modulating neuronal activities of the superficial laminae of the spinal cord and certain regions of the brain.

关键词

Keywords

electroacupuncturemyeloid differentiation factor 2inflammation-induced paindorsal hornperiaqueductal graylocus coeruleus

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