Effect of Bushen Huoxue Granule on Clearance of Pathological α-Synuclein in MPP<sup>+</sup>-Induced PC12 Cells

LUAN Zhen-xian; TANG Xiang-lin; HAO Fei-ran; LI Min; LI Shao-dan; YANG Ming-hui

doi:10.1007/s11655-025-3707-2

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Effect of Bushen Huoxue Granule on Clearance of Pathological α-Synuclein in MPP⁺-Induced PC12 Cells

Original Article | Updated：2025-08-25

- Effect of Bushen Huoxue Granule on Clearance of Pathological α-Synuclein in MPP⁺-Induced PC12 Cells
- Effect of Bushen Huoxue Granule on Clearance of Pathological α-Synuclein in MPP⁺-Induced PC12 Cells
- 中国结合医学杂志（英文版） 2025年31卷第9期页码：830-836
- Affiliations：
  
  Medical Department of Traditional Chinese Medicine, the Sixth Medical Center of the PLA General Hospital, Beijing (100048), China
- Author bio：
  
  Prof. YANG Ming-hui, E-mail: Ymh9651@sina.com
- Funds：
  
  the National Natural Science Foundation of China(82130117)
- DOI：10.1007/s11655-025-3707-2
  中图分类号：
- 录用日期：2024-05-05，
  
  网络出版日期：2025-03-25，
  
  纸质出版日期：2025-09
- Accepted：
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Effect of Bushen Huoxue Granule on Clearance of Pathological α-Synuclein in MPP⁺-Induced PC12 Cells[J]. 中国结合医学杂志（英文版）, 2025,31(9):830-836.

LUAN Zhen-xian, TANG Xiang-lin, HAO Fei-ran, et al. Effect of Bushen Huoxue Granule on Clearance of Pathological α-Synuclein in MPP⁺-Induced PC12 Cells[J]. Chinese journal of integrative medicine, 2025, 31(9): 830-836.
Effect of Bushen Huoxue Granule on Clearance of Pathological α-Synuclein in MPP⁺-Induced PC12 Cells[J]. 中国结合医学杂志（英文版）, 2025,31(9):830-836. DOI： 10.1007/s11655-025-3707-2.

LUAN Zhen-xian, TANG Xiang-lin, HAO Fei-ran, et al. Effect of Bushen Huoxue Granule on Clearance of Pathological α-Synuclein in MPP⁺-Induced PC12 Cells[J]. Chinese journal of integrative medicine, 2025, 31(9): 830-836. DOI： 10.1007/s11655-025-3707-2.

摘要

Abstract

Objective:

To investigate the effects of Bushen Huoxue Granule on the ubiquitin-proteasome system (UPS) in an

in vitro

model of Parkinson's disease.

Methods:

After treated with 1-methyl-4-phenylpyridinium (MPP

1 mmol/L) for 24 h

the cells were incubated with drug-free serum

Madopar-containing serum or Bushen Huoxue Granule-containing serum (BCS

10%

and 20%) for another 24 h. The levels of α-synuclein (α-syn)

tyrosine hydroxylase (TH) and UPS-related proteins were detected by Western blot. The expression levels of α-syn in PC12 cells were also analyzed by Western blot after treated with proteasome inhibitor MG132 and WT-α-syn plasmid transfection

respectively

as well as the alterations induced by subsequent BCS intervention. Immunocytochemistry was performed to determine the changes in α-syn phosphorylation at serine 129 (pSer129-α-syn) expression. The 20S proteasome levels were measured by enzyme-linked immunosorbnent assay.

Results:

BCS (volume fraction 20%) intervention could alleviate the MMP

-induced cell viability decrease (

0.05). In the MPP

treated cells

α-syn was up-regulated

while TH and proteins of UPS such as ubiquitin (Ub)

Ub binding with Ub-activating enzyme (UBE1)

Parkin and Ub C-terminal hydrolase-1 (UCHL-1) were down-regulated (

0.05). BCS intervention could attenuate the above changes (

0.05). The activity of BCS on blocking α-syn accumulation was weakened by MG132 (

0.05). While α-syn level was significantly increased in cells transfected with plasmid

and reduced by BCS intervention (

0.05). pSer129-α-syn was increased in MPP

-induced PC12 cells

whereas decreased by later BCS intervention (

0.05). The 20S proteasome activity of MPP

-induced PC12 cells was decreased

but increased after BCS intervention (

0.05).

Conclusion:

BCS intervention protected UPS function

increased 20S proteasome activity

promoted pathological α-syn clearance

restored cell viability

and reversed the damage caused by MPP

in the

in vitro

model of Parkinson's disease.

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Effect of Bushen Huoxue Granule on Clearance of Pathological α-Synuclein in MPP+-Induced PC12 Cells

Effect of Bushen Huoxue Granule on Clearance of Pathological α-Synuclein in MPP+-Induced PC12 Cells

DOI：10.1007/s11655-025-3707-2

摘要

Abstract

关键词

Keywords

references

Effect of Bushen Huoxue Granule on Clearance of Pathological α-Synuclein in MPP⁺-Induced PC12 Cells

Effect of Bushen Huoxue Granule on Clearance of Pathological α-Synuclein in MPP⁺-Induced PC12 Cells