Salvianolic Acid B Exerts Antiphotoaging Effect on Ultraviolet B-Irradiated Human Keratinocytes by Alleviating Oxidative Stress via SIRT1 Protein<sup>*</sup>

ZHANG Qiao-ju; LUO Xi; ZHENG Yu-wen; ZHENG Jun-qiao; WU Xin-ying; WANG Shu-mei; SHI Jun

doi:10.1007/s11655-025-3931-9

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Salvianolic Acid B Exerts Antiphotoaging Effect on Ultraviolet B-Irradiated Human Keratinocytes by Alleviating Oxidative Stress via SIRT1 Protein^*

Original Article | Updated：2025-10-23

- Salvianolic Acid B Exerts Antiphotoaging Effect on Ultraviolet B-Irradiated Human Keratinocytes by Alleviating Oxidative Stress via SIRT1 Protein^*
- Salvianolic Acid B Exerts Antiphotoaging Effect on Ultraviolet B-Irradiated Human Keratinocytes by Alleviating Oxidative Stress via SIRT1 Protein^*
- 中国结合医学杂志（英文版） 2025年31卷第11期页码：1021-1028
- Affiliations：
  
  1.School of Chinese Materia Medica, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou (510006), China
  2.College of Traditional Chinese Medicine, Huizhou Health Sciences Polytechnic, Huizhou, Guangdong Province (516025), China
  3.Guangdong Provincial Key Laboratory for Research and Evaluation of Pharmaceutical Preparations, Guangzhou (510006), China
- Author bio：
  
  Prof. SHI Jun, E-mail: shijun8008@163.com
- Funds：
  
  the National Natural Science Foundation of China(82173982);the Guangdong Natural Science Project(2022A1515011382)
- DOI：10.1007/s11655-025-3931-9
  中图分类号：
- 录用：2024-12-10，
  
  纸质出版：2025-11
- Accepted：
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Salvianolic Acid B Exerts Antiphotoaging Effect on Ultraviolet B-Irradiated Human Keratinocytes by Alleviating Oxidative Stress via SIRT1 Protein^*[J]. 中国结合医学杂志（英文版）, 2025,31(11):1021-1028.

ZHANG Qiao-ju, LUO Xi, ZHENG Yu-wen, et al. Salvianolic Acid B Exerts Antiphotoaging Effect on Ultraviolet B-Irradiated Human Keratinocytes by Alleviating Oxidative Stress via SIRT1 Protein^*[J]. Chinese journal of integrative medicine, 2025, 31(11): 1021-1028.
Salvianolic Acid B Exerts Antiphotoaging Effect on Ultraviolet B-Irradiated Human Keratinocytes by Alleviating Oxidative Stress via SIRT1 Protein^*[J]. 中国结合医学杂志（英文版）, 2025,31(11):1021-1028. DOI： 10.1007/s11655-025-3931-9.

ZHANG Qiao-ju, LUO Xi, ZHENG Yu-wen, et al. Salvianolic Acid B Exerts Antiphotoaging Effect on Ultraviolet B-Irradiated Human Keratinocytes by Alleviating Oxidative Stress via SIRT1 Protein^*[J]. Chinese journal of integrative medicine, 2025, 31(11): 1021-1028. DOI： 10.1007/s11655-025-3931-9.

摘要

Abstract

Objective:

To explore the anti-photoaging properties of salvianolic acid B (Sal B).

Methods:

The optimal photoaging model of human immortalized keratinocytes (HaCaT cells) were constructed by expose to ultraviolet B (UVB) radiation. The cells were divided into control

model and different concentrations of Sal B groups. Cell viability was measured via cell counting kit-8. Subsequently

the levels of oxidative stress

including reactive oxygen species (ROS)

hydroxyproline (Hyp)

catalase (CAT)

and glutathione peroxidase (GSH-Px) were detected using the relevant kits. Silent information regulator 1 (SIRT1) protein level was detected using Western blot. The binding pattern of Sal B and SIRT1 was determined via molecular docking.

Results:

Sal B significantly increased the viability of UVB-irradiated HaCaT cells (

0.05 or

0.01). Sal

B effectively scavenged the accumulation of ROS induced by UVB (

0.05 or

0.01). In addition

Sal B modulated oxidative stress by increasing the intracellular concentrations of Hyp and CAT and the activity of GSH-Px (

0.05 or

0.01). The Western blot results revealed a substantial increase in SIRT1 protein levels following Sal B administration (

0.05). Moreover

Sal B exhibited good binding affinity toward SIRT1

with a docking energy of –7.5 kCal/mol.

Conclusion:

Sal B could improve the repair of photodamaged cells by alleviating cellular oxidative stress and regulating the expression of SIRT1 protein.

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