Honeysuckle-Derived Exosome-Like Nanovesicles Alleviate Cisplatin-Induced Acute Kidney Injury by Anti-Inflammation and Mitochondrial Function Protection

GONG Wan-cheng; LI Jing-xuan; HU Xiao-juan; HUANG Yi-lin; WU Ai-lin; LI Yi-shu; WANG Xin-qiu; ZHANG Xing-xian; MOU Xiao-zhou; YANG Xiang-hong

doi:10.1007/s11655-025-4026-3

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Honeysuckle-Derived Exosome-Like Nanovesicles Alleviate Cisplatin-Induced Acute Kidney Injury by Anti-Inflammation and Mitochondrial Function Protection

Original Article | Updated：2026-02-14

- Honeysuckle-Derived Exosome-Like Nanovesicles Alleviate Cisplatin-Induced Acute Kidney Injury by Anti-Inflammation and Mitochondrial Function Protection
- Honeysuckle-Derived Exosome-Like Nanovesicles Alleviate Cisplatin-Induced Acute Kidney Injury by Anti-Inflammation and Mitochondrial Function Protection
- Chinese Journal of Integrative Medicine 2026年32卷第1期页码：34-42
- Affiliations：
  
  1.College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou (310014), China
  2.Emergency and Critical Care Center, Intensive Care Unit, Zhejiang Provincial People's Hospital, Hangzhou Medical College, Hangzhou (310014), China
  3.Clinical Research Institute, Zhejiang Provincial People's Hospital, Hangzhou Medical College, Hangzhou (310014), China
- Author bio：
  
  Prof. YANG Xiang-hong, E-mail: jyy623@163.com
- Funds：
  
  Provincial Key R&D Program Project of Zhejiang Provincial Science and Technology Department(2019C03024)
- DOI：10.1007/s11655-025-4026-3
  中图分类号：
- 录用：2025-05-08，
  
  网络首发：2025-11-18，
  
  纸质出版：2026-01
- Accepted：
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GONG Wan-cheng, LI Jing-xuan, HU Xiao-juan, 等. Honeysuckle-Derived Exosome-Like Nanovesicles Alleviate Cisplatin-Induced Acute Kidney Injury by Anti-Inflammation and Mitochondrial Function Protection[J]. Chinese Journal of Integrative Medicine, 2026,32(1):34-42.

GONG Wan-cheng, LI Jing-xuan, HU Xiao-juan, et al. Honeysuckle-Derived Exosome-Like Nanovesicles Alleviate Cisplatin-Induced Acute Kidney Injury by Anti-Inflammation and Mitochondrial Function Protection[J]. Chinese Journal of Integrative Medicine, 2026, 32(1): 34-42.
GONG Wan-cheng, LI Jing-xuan, HU Xiao-juan, 等. Honeysuckle-Derived Exosome-Like Nanovesicles Alleviate Cisplatin-Induced Acute Kidney Injury by Anti-Inflammation and Mitochondrial Function Protection[J]. Chinese Journal of Integrative Medicine, 2026,32(1):34-42. DOI： 10.1007/s11655-025-4026-3.

GONG Wan-cheng, LI Jing-xuan, HU Xiao-juan, et al. Honeysuckle-Derived Exosome-Like Nanovesicles Alleviate Cisplatin-Induced Acute Kidney Injury by Anti-Inflammation and Mitochondrial Function Protection[J]. Chinese Journal of Integrative Medicine, 2026, 32(1): 34-42. DOI： 10.1007/s11655-025-4026-3.

摘要

Abstract

Objective:

To explore the potential of honeysuckle-derived exosome-like nanovesicles (HELNVs) for preventing cisplatin-induced acute kidney injury (AKI).

Methods:

The renoprotective efficacy of HELNVs against cisplatin-induced AKI was assessed in human kidney cell-2 (HK-2) cells exposed to 100 μmol/L cisplatin for 24 h

followed by HELNVs (50–200 μg/mL) for another 24 h; the optimal therapeutic concentration was determined as 100 μg/mL. At this concentration

oxygen species (ROS) levels were measured by flow cytometry. Male C57BL/6 mice received a single intraperitoneal injection of cisplatin (30 mg/kg) to establish an AKI model and were then computer-randomized into 3 groups (

=6 per group): control group

daily intraperitoneal administration of normal saline (0.9% NaCl); the cisplatin-injured group

same NaCl regimen; the HELNVs treatment group

daily intraperitoneal administration of HELNVs (30 mg/kg) for 5 consecutive days

with euthanasia on day 6. Renal accumulation of HELNVs was tracked by small-animal multispectral imaging (peak uptake at 8–10 h). Functional assessment included serum creatinine and blood urea nitrogen (BUN) quantified with an automated biochemical analyzer. Molecular analyses included enzyme-linked immunosorbent assay (ELISA) quantification of interleukin (IL)-1β

IL-6

IL-10

and tumor necrosis factor-α (TNF-α).

Results:

HELNVs significantly reduced ROS

inflammatory responses

and apoptosis in cisplatin-treated HK-2 cells (

0.01). In cisplatin-induced AKI mice

HELNVs were efficiently absorbed by kidney cells

effectively prevented oxidative damage and mitochondrial dysfunction (

0.01). Following treatment with 30 mg/kg HELNVs

serum creatinine and BUN levels were markedly reduced (

0.01). ELISA results showed decreased levels of IL-1β

IL-6

and TNF-α

along with up-regulated IL-10 (

0.01).

Conclusion:

HELNVs may serve as a promising therapeutic approach for ameliorating cisplatin-induced AKI

offering a potential novel treatment option for managing this condition in clinical settings.

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