Protective effects and mechanism of puerarin on learning-memory disorder after global cerebral ischemia-reperfusion injury in rats

Hai-qin Wu; He-na Guo; Hu-qing Wang; Ming-ze Chang; Gui-lian Zhang; Ying-xian Zhao

doi:10.1007/s11655-009-0054-4

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Protective effects and mechanism of puerarin on learning-memory disorder after global cerebral ischemia-reperfusion injury in rats

Updated：2021-08-27

- Protective effects and mechanism of puerarin on learning-memory disorder after global cerebral ischemia-reperfusion injury in rats
- Chinese Journal of Integrative Medicine Vol. 15, Issue 1, Pages: 54-59(2009)
- Affiliations：
  
  Department of Neurology, the Second Affiliated Hospital, Medical College of Xi’an Jiaotong University,Xi’an,China
- Author bio：
- Funds：
  
  Supported by the Key Technologies Research and Development Program of Shaanxi Province (No. 2002k10-G2)
- DOI：10.1007/s11655-009-0054-4
  CLC：
- Published：2009，
  
  Published Online：7 March 2009，
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Wu, Hq., Guo, Hn., Wang, Hq. et al. Protective effects and mechanism of puerarin on learning-memory disorder after global cerebral ischemia-reperfusion injury in rats., Chin. J. Integr. Med. 15, 54–59 (2009). https://doi.org/10.1007/s11655-009-0054-4

Hai-qin Wu, He-na Guo, Hu-qing Wang, et al. Protective effects and mechanism of puerarin on learning-memory disorder after global cerebral ischemia-reperfusion injury in rats. [J]. Chinese Journal of Integrative Medicine 15(1):54-59(2009)
Wu, Hq., Guo, Hn., Wang, Hq. et al. Protective effects and mechanism of puerarin on learning-memory disorder after global cerebral ischemia-reperfusion injury in rats., Chin. J. Integr. Med. 15, 54–59 (2009). https://doi.org/10.1007/s11655-009-0054-4 DOI：

Hai-qin Wu, He-na Guo, Hu-qing Wang, et al. Protective effects and mechanism of puerarin on learning-memory disorder after global cerebral ischemia-reperfusion injury in rats. [J]. Chinese Journal of Integrative Medicine 15(1):54-59(2009) DOI： 10.1007/s11655-009-0054-4.

摘要

To observe the effect of puerarin on the learning-memory disorder after global cerebral ischemia-reperfusion injury in rats

and to explore its mechanism of action. The global cerebral ischemia-reperfusion injury model was established using the modifified Pulsinelli four-vessel occlusion in Sprague-Dawley rats. Rats were intraperitoneally injected with puerarin (100 mg/kg) 1 h before ischemia and once every 6 h afterwards. The learning-memory ability was evaluated by the passive avoidance test. The dynamic changes of the cell counts of apoptosis and positive expression of Bcl-2 in the hippocampus CA1 region were determined by the TUNEL and immunohistochemical methods

respectively. (1) Compared with the reperfusion group

the step through latency (STL) in the passive avoidance test in the puerarin group was prolonged signifificantly (P<0.01). (2) The apoptotic neurons were injured most severely on the 3rd day in the hippocampal CA1 region after global ischemia and reperfusion. In the puerarin group

the number of apoptotic cells decreased at respective time points after ischemia-reperfusion (P<0.01). (3) The level of positive expression of Bcl-2 varied according to the duration of reperfusion and the peak level occurred on day 1 in the hippocampal CA1 region after global cerebral ischemia. Compared with the reperfusion group

the expression of Bcl-2 in the puerarin group was up-regulated at the respective time points after ischemia reperfusion (P<0.01)

reaching the peak on day 1. Puerarin could improve the learning-memory ability after global cerebral ischemia and reperfusion in rats. The protective mechanism might be related to the effect of inhibiting or delaying the cell apoptosis through up-regulating the expression of Bcl-2 after ischemia and reperfusion.

Abstract

To observe the effect of puerarin on the learning-memory disorder after global cerebral ischemia-reperfusion injury in rats

respectively. (1) Compared with the reperfusion group

the expression of Bcl-2 in the puerarin group was up-regulated at the respective time points after ischemia reperfusion (P<0.01)

关键词

Puerarincerebral ischemia-reperfusion injurylearning-memory disorder

Keywords

Puerarincerebral ischemia-reperfusion injurylearning-memory disorder

references

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Related Institution

Deparment of Laboratory Medicine, Wenzhou Medical College

Department of Clinical Laboratory, Chinese People’s Liberation Army General Hospital

Institute of Basic Medical Sciences, Peking Union Medical College, Chinese Academy of Medical Sciences

Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences

Department of Orthopedics, the Second Affiliated Hospital of Medical College of Xi’an Jiaotong University

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