Effect of serum from overfatigue rats on JNK/c-Jun/HO-1 pathway in human umbilical vein endothelial cells and the intervening effect of Tongxinluo (通心络) superfine powder

Jun-qing Liang; Hai-bo Xu; Yi-ling Wu; Shi-ran Sun; Zhen-hua Jia; Cong Wei; Jia-hua You

doi:10.1007/s11655-009-0121-0

Your Location：

Home >

Browse articles >

Effect of serum from overfatigue rats on JNK/c-Jun/HO-1 pathway in human umbilical vein endothelial cells and the intervening effect of Tongxinluo (通心络) superfine powder

Updated：2021-08-27

- Effect of serum from overfatigue rats on JNK/c-Jun/HO-1 pathway in human umbilical vein endothelial cells and the intervening effect of Tongxinluo (通心络) superfine powder
- Chinese Journal of Integrative Medicine Vol. 15, Issue 2, Pages: 121-127(2009)
- Affiliations：
  
  1. Department of Pharmacology, Hebei Yiling Pharmaceutical Research Institute,Shijiazhuang,China
  2. Synthesized Department of the Affiliated Hospital of Chengde Medical College,Chengde,China
  3. Department of Gastroenterology, Traditional Chinese Medicine Hospital of Hebei,Shijiazhuang,China
  4. Department of Neurology, Traditional Chinese Medicine Hospital of Shijiazhuang,Shijiazhuang,China
- Author bio：
- Funds：
  
  Supported by the National Key Basic Research and Development Project (973 Project, No. 2005CB523301), the International Science and Technology Cooperation Program (No. 2006DFB32460)
- DOI：10.1007/s11655-009-0121-0
  CLC：
- Published：2009，
  
  Published Online：29 April 2009，
Scan for full text
Liang, Jq., Xu, Hb., Wu, Yl. et al. Effect of serum from overfatigue rats on JNK/c-Jun/HO-1 pathway in human umbilical vein endothelial cells and the intervening effect of Tongxinluo (通心络) superfine powder., Chin. J. Integr. Med. 15, 121–127 (2009). https://doi.org/10.1007/s11655-009-0121-0

Jun-qing Liang, Hai-bo Xu, Yi-ling Wu, et al. Effect of serum from overfatigue rats on JNK/c-Jun/HO-1 pathway in human umbilical vein endothelial cells and the intervening effect of Tongxinluo (通心络) superfine powder. [J]. Chinese Journal of Integrative Medicine 15(2):121-127(2009)
Liang, Jq., Xu, Hb., Wu, Yl. et al. Effect of serum from overfatigue rats on JNK/c-Jun/HO-1 pathway in human umbilical vein endothelial cells and the intervening effect of Tongxinluo (通心络) superfine powder., Chin. J. Integr. Med. 15, 121–127 (2009). https://doi.org/10.1007/s11655-009-0121-0 DOI：

Jun-qing Liang, Hai-bo Xu, Yi-ling Wu, et al. Effect of serum from overfatigue rats on JNK/c-Jun/HO-1 pathway in human umbilical vein endothelial cells and the intervening effect of Tongxinluo (通心络) superfine powder. [J]. Chinese Journal of Integrative Medicine 15(2):121-127(2009) DOI： 10.1007/s11655-009-0121-0.

摘要

To cultivate human umbilical vein endothelial cells (HUVECs) in the serum of overfatigue rats with the intervention of Tongxinluo (通心络) superfine powder (TXLSP). By examining the variation of the activity of JNK/c-Jun/HO-1 pathway

the possible mechanisms of vascular endothelial dysfunction under overfatigue conditions and the intervening effect of TXLSP were explored. The HUVECs were randomly divided into the normal control group

the model group

the SP600125 (a specifific antagonist of JNK) group

the TXLSP group and the TXLSP + SP600125 group. The content of carboyhemoglobin (COHb) and the leak rate of lactic dehydrogenase (LDH) in different groups were measured. The mRNA and protein expression of JNK

c-Jun

HO-1 and the phosphorylation level of c-Jun (P-c-Jun) were detected using Western blot and PCR methods. Compared with the normal control group

the COHb level in supernatant was increased signifificantly in the model group

and the expression of HO-1

JNK

c-Jun mRNA and corresponding proteins and P-c-Jun were also increased remarkably. The increases in these parameters were signifificantly decreased by SP600125. TXLSP showed remarkable up-regulation on the expression of JNK

c-Jun

P-c-Jun and HO-1 mRNA and their protein expression. Compared with the SP600125 group

the expressions of JNK

c-Jun

P-c-Jun and HO-1 mRNA and its protein in the TXLSP+SP600125 group were signifificantly increased at different time points (P<0.05

P<0.01). The vascular endothelial dysfunction under overfatigue conditions is related to the activity of the JNK/c-Jun/HO-1 pathway. One of the mechanisms of TXLSP in improving the vascular endothelial function is to adjust the activity of the JNK/c-Jun/HO-1 pathway at gene and protein levels.

Abstract

the model group

the SP600125 (a specifific antagonist of JNK) group

c-Jun

HO-1 and the phosphorylation level of c-Jun (P-c-Jun) were detected using Western blot and PCR methods. Compared with the normal control group

the COHb level in supernatant was increased signifificantly in the model group

and the expression of HO-1

JNK

c-Jun

P-c-Jun and HO-1 mRNA and their protein expression. Compared with the SP600125 group

the expressions of JNK

c-Jun

P-c-Jun and HO-1 mRNA and its protein in the TXLSP+SP600125 group were signifificantly increased at different time points (P<0.05

关键词

overfatigueserumvascular endothelial dysfunctionsignal conductionTongxinluo superfine powder

Keywords

overfatigueserumvascular endothelial dysfunctionsignal conductionTongxinluo superfine powder

references

Wu YL. The study of relevance between collaterals and vascular disease and treatment. J Tradit Chin Med (Chin) 2006;47:163–165.

Cheng ZQ, Yao L, Gong WB, Tang YX, Wu YF. The establishment and appraisal of Wistar rat with heart-qi deficiency syndrome. China J Tradit Chin Med Pharm (Chin) 2003;18:654–658.

Morita T, Perrella MA, Lee ME, Kourembanas S. Smooth muscle cell-derived carbon monoxide is a regulator of vascular cGMP. Proc Natl Acad Sci USA 1995;92:1475–1479.

Liang JQ, Wu YL, Jia ZH, Xu HB, Ding CH, Wang HT. The changes of HO/CO system in arterial tissuse of rats with collateral-qi in a defficiency condition and intervening effects of renshen, shuangshen and TXL. Second Milit Med Univ J (Chin) 2007;28:782–785.

Guo YZ, Chen HP. Effects of hemin on the expression of hemeoxygenase in human umbilical vein endothelial cells. J Chin Phys (Chin) 2006;8:173–175.

Adams JL, Badger AM, Kumar S, Lee JC. p38 MAP kinase: molecular target for the inhibition of pro-inflammatory cytokines. Prog Med Chem 2001;38:1–60.

Han J, Lee JD, Bibbs L, Ulevitch RJ. A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells. Science 1994;265:808–811.

Ono K, Han J. The p38 signal transduction pathway activation and function. Cell Signal 2000;12:1–13.

Lee PJ, Camhi SL, Chin BY, Alam J, Choi AM. AP-1 and STAT mediate hyperoxia-induced gene transcription of heme oxygenase-1. Am J Physiol Lung Cell Mol Physiol 2000;279:175–182.

Ryseck RP, Bravo R. c-Jun, Jun B, and Jun D differ in their binding affinities to AP-1 and CRE consensus sequences: effect of FOS proteins. Oncogene 1991;6:533–542.

Bulger EM, Garcia I, Maier RV. Induction of heme oxygenase-1 inhibits endothelial cell activation by endotoxin and oxidant stress. Surgery 2003;134:146–152.

Wu YL. Collateral disease theory. Beijing: Chinese Science and Technology Publishing House; 2004:864–867.

FULL TEXT LINK

More>

Views

382

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

No data

Related Author

No data

Related Institution

No data

Address：1 Caochang, xiyuan, Beijing, People's Republic of China Postal code：100091
Tel：010-62886827, 62877592 Fax：010-62874291
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05067934号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰