Ginsenoside Rb1, a panoxadiol saponin against oxidative damage and renal interstitial fibrosis in rats with unilateral ureteral obstruction

Xi-sheng Xie; Heng-chuan Liu; Man Yang; Chuan Zuo; Yao Deng; Jun-ming Fan

doi:10.1007/s11655-009-0133-9

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Ginsenoside Rb1, a panoxadiol saponin against oxidative damage and renal interstitial fibrosis in rats with unilateral ureteral obstruction

Updated：2021-08-27

- Ginsenoside Rb1, a panoxadiol saponin against oxidative damage and renal interstitial fibrosis in rats with unilateral ureteral obstruction
- Chinese Journal of Integrative Medicine Vol. 15, Issue 2, Pages: 133-140(2009)
- Affiliations：
  
  1. Department of Nephrology, West China Hospital, Sichuan University,Chengdu,China
  2. Department of Medicine Technology, West China School of Public Health, Sichuan University,Chengdu,China
  3. State Key Laboratory of Biotherapy of Human Disease, West China Hospital, Sichuan University,Chengdu,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-009-0133-9
  CLC：
- Published：2009，
  
  Published Online：29 April 2009，
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Xie, Xs., Liu, Hc., Yang, M. et al. Ginsenoside Rb1, a panoxadiol saponin against oxidative damage and renal interstitial fibrosis in rats with unilateral ureteral obstruction., Chin. J. Integr. Med. 15, 133–140 (2009). https://doi.org/10.1007/s11655-009-0133-9

Xi-sheng Xie, Heng-chuan Liu, Man Yang, et al. Ginsenoside Rb1, a panoxadiol saponin against oxidative damage and renal interstitial fibrosis in rats with unilateral ureteral obstruction. [J]. Chinese Journal of Integrative Medicine 15(2):133-140(2009)
Xie, Xs., Liu, Hc., Yang, M. et al. Ginsenoside Rb1, a panoxadiol saponin against oxidative damage and renal interstitial fibrosis in rats with unilateral ureteral obstruction., Chin. J. Integr. Med. 15, 133–140 (2009). https://doi.org/10.1007/s11655-009-0133-9 DOI：

Xi-sheng Xie, Heng-chuan Liu, Man Yang, et al. Ginsenoside Rb1, a panoxadiol saponin against oxidative damage and renal interstitial fibrosis in rats with unilateral ureteral obstruction. [J]. Chinese Journal of Integrative Medicine 15(2):133-140(2009) DOI： 10.1007/s11655-009-0133-9.

摘要

To investigate the possible protective effect and mechanism of ginsenoside Rb1 against oxidative damage and renal interstitial fibrosis on rats with unilateral ureteral obstruction (UUO). In total

80 male rats were randomly divided into 4 groups

20 in each group: the sham operated group (SOR)

UUO group

UUO with ginsenoside Rb1 treatment group (treated with intraperitoneal injection of 50 mg/ kg daily) and UUO with Losartan treatment group (as the positive control

treated with 20 mg/kg by gastrogavage per day). The rats were randomly sacrifificed on day 3

7 and 14 after surgery

respectively. The histopathologic changes of renal interstitial tissues were observed with Masson staining. The mRNA of transforming growth factor β 1 (TGF-β 1)

collagen I and fibronectin were reversed transcribed and quantified by Real-time PCR. Enzyme-linked immunosorbent assay was used to quantitatively detect TGF-β 1 and 8-hydroxy-2′-deoxyguanosine (8-OHdG) levels. P47phox protein expression was assessed by immunohistochemistry and Western blot analysis. In the UUO model

the obstructed kidney showed typical features of progressive renal tubulointerstitial fibrosis

and the levels of TGF-β1

collagen I and fibronectin increased (P<0.05). As compared with the UUO group

ginsennoside Rb1 significantly inhibited the interstitial fibrosis including tubular injury and collagen deposition

and decreased the levels of TGF-β1 (P<0.05). Ginsenoside Rb1 also inhibited the heme oxygenase (HO-1) and 8-OHdG

two markers of oxidative stress (P<0.05). Moreover

ginsenoside Rb1 suppressed the expression of p47phox

a subunit of nicotinamide adeninedinucleotide phosphate (NADPH) oxidase (P<0.05). Ginsenoside Rb1 can obviously inhibit renal interstitial fibrosis in rats with UUO

its mechanism possibly via against the oxidative damage and suppressing TGF-β1 expression.

Abstract

To investigate the possible protective effect and mechanism of ginsenoside Rb1 against oxidative damage and renal interstitial fibrosis on rats with unilateral ureteral obstruction (UUO). In total

80 male rats were randomly divided into 4 groups

20 in each group: the sham operated group (SOR)

UUO group

UUO with ginsenoside Rb1 treatment group (treated with intraperitoneal injection of 50 mg/ kg daily) and UUO with Losartan treatment group (as the positive control

treated with 20 mg/kg by gastrogavage per day). The rats were randomly sacrifificed on day 3

7 and 14 after surgery

respectively. The histopathologic changes of renal interstitial tissues were observed with Masson staining. The mRNA of transforming growth factor β 1 (TGF-β 1)

the obstructed kidney showed typical features of progressive renal tubulointerstitial fibrosis

and the levels of TGF-β1

collagen I and fibronectin increased (P<0.05). As compared with the UUO group

ginsennoside Rb1 significantly inhibited the interstitial fibrosis including tubular injury and collagen deposition

and decreased the levels of TGF-β1 (P<0.05). Ginsenoside Rb1 also inhibited the heme oxygenase (HO-1) and 8-OHdG

two markers of oxidative stress (P<0.05). Moreover

ginsenoside Rb1 suppressed the expression of p47phox

a subunit of nicotinamide adeninedinucleotide phosphate (NADPH) oxidase (P<0.05). Ginsenoside Rb1 can obviously inhibit renal interstitial fibrosis in rats with UUO

its mechanism possibly via against the oxidative damage and suppressing TGF-β1 expression.

关键词

ginsenoside Rb1unilateral ureteral obstructionoxidative stresstransforming growth factor beta1p47phox

Keywords

ginsenoside Rb1unilateral ureteral obstructionoxidative stresstransforming growth factor beta1p47phox

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