Synergistic anti-inflammatory effect of Radix Platycodon in combination with herbs for cleaning-heat and detoxification and its mechanism

Yu-hang Li; Feng-jie Zheng; Ying Huang; Xiang-gen Zhong; Ming-zhang Guo

doi:10.1007/s11655-011-0763-6

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Synergistic anti-inflammatory effect of Radix Platycodon in combination with herbs for cleaning-heat and detoxification and its mechanism

Updated：2021-08-27

- Synergistic anti-inflammatory effect of Radix Platycodon in combination with herbs for cleaning-heat and detoxification and its mechanism
- Chinese Journal of Integrative Medicine Vol. 19, Issue 1, Pages: 29-35(2013)
- Affiliations：
  
  1. Biomedical Research School, Beijing University of Chinese Medicine,Beijing,China
  2. Experimental Center, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Basic Research Program of China (973 Program, No. 2009CB522704) and the National Natural Science Foundation of China (No. 30672676)
- DOI：10.1007/s11655-011-0763-6
  CLC：
- Published：2013，
  
  Published Online：29 June 2011，
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Li, Yh., Zheng, Fj., Huang, Y. et al. Synergistic anti-inflammatory effect of Radix Platycodon in combination with herbs for cleaning-heat and detoxification and its mechanism., Chin. J. Integr. Med. 19, 29–35 (2013). https://doi.org/10.1007/s11655-011-0763-6

Yu-hang Li, Feng-jie Zheng, Ying Huang, et al. Synergistic anti-inflammatory effect of Radix Platycodon in combination with herbs for cleaning-heat and detoxification and its mechanism. [J]. Chinese Journal of Integrative Medicine 19(1):29-35(2013)
Li, Yh., Zheng, Fj., Huang, Y. et al. Synergistic anti-inflammatory effect of Radix Platycodon in combination with herbs for cleaning-heat and detoxification and its mechanism., Chin. J. Integr. Med. 19, 29–35 (2013). https://doi.org/10.1007/s11655-011-0763-6 DOI：

Yu-hang Li, Feng-jie Zheng, Ying Huang, et al. Synergistic anti-inflammatory effect of Radix Platycodon in combination with herbs for cleaning-heat and detoxification and its mechanism. [J]. Chinese Journal of Integrative Medicine 19(1):29-35(2013) DOI： 10.1007/s11655-011-0763-6.

摘要

To investigate the synergistic anti-inflammatory effect of Radix Platycodon in combination with herbs for cleaning-heat and detoxification and its mechanism for Fei (肺)-targeting. Forty Wistar rats were randomly divided into five groups (8 per group): the sham-operated group

model group

Radix Platycodon group

Flos Lonicera and Fructus Forsythia (LF) group

and Radix Platycodon

Flos Lonicera and Fructus Forsythia combination (PLF) group

using a random number table. A rat chronic obstructive pulmonary disease (COPD) model was established by passive smoking and intratracheal instillation of lipopolysaccharide (LPS). The treatments started from the 15th day of passive smoking for a total duration of 14 days. At the end of the treatment

changes in the following measurements were determined: lung histopathology

inflammatory cytokines including tumor necrosis factor α (TNF-α)

transforming growth factor β (TGF-β) and interleukin IL-1β (IL-1β) in bronchoalveolar lavage fluid (BALF)

and mRNA expression of endogenous active substance intestinal trefoil factor 3 (TFF3) in the lung tissue. Light microscopy showed that compared with the sham-operated group

rats in the COPD model group had disrupted alveolar structure

collapsed local alveoli

significantly widened or even fused alveolar septa

and massive infiltration of inflammatory cells in the alveolar wall and interstitium. In addition

significant bronchial epithelium hyperplasia

partially shed epithelia

and marked inflammatory cell infiltration in the bronchial wall and its surrounding tissues were noticed. Electron microscopy showed that rats in the model group had degeneration of alveolar type II epithelial cell; reduction

breakage or even loss of cell surface microvilli; swollen mitochondria with disappearing cristae and vacuole-like structure; and

increased secondary lysosomes in alveolar macrophages. The TNF-α

TGF-β and IL-1β levels and white blood cell (WBC) count in BALF were significantly increased (P<0.01 or P<0.05) and TFF3 mRNA expression in the lung tissue was significantly reduced (P<0.01). After treatment

the pathological morphology of lung injury was less severe in all three treatment groups. In addition

TGF-β and IL-1β and WBC count in BALF were decreased (P<0.01 or P<0.05)

and TFF3 mRNA expression in the lung tissue was significantly increased in the PLF group (P<0.01). Compared with the LF group

the IL-1β in BALF was significantly decreased P<0.05)

and TFF3 mRNA expression was significantly increased (P<0.05) in the PLF group. Radix Platycodon synergizes with herbs for cleaning-heat and detoxification in reducing inflammatory injury in a rat model of COPD. The synergistic anti-inflammatory effect is reflected in the improvement in pathological changes and in the reduction of IL-1β levels in BALF. The mechanism of such synergistic action may be related to its effect on maintaining the TFF3 mRNA expression and Fei-targeting function.

Abstract

model group

Radix Platycodon group

Flos Lonicera and Fructus Forsythia (LF) group

and Radix Platycodon

Flos Lonicera and Fructus Forsythia combination (PLF) group

changes in the following measurements were determined: lung histopathology

inflammatory cytokines including tumor necrosis factor α (TNF-α)

transforming growth factor β (TGF-β) and interleukin IL-1β (IL-1β) in bronchoalveolar lavage fluid (BALF)

and mRNA expression of endogenous active substance intestinal trefoil factor 3 (TFF3) in the lung tissue. Light microscopy showed that compared with the sham-operated group

rats in the COPD model group had disrupted alveolar structure

collapsed local alveoli

significantly widened or even fused alveolar septa

and massive infiltration of inflammatory cells in the alveolar wall and interstitium. In addition

significant bronchial epithelium hyperplasia

partially shed epithelia

breakage or even loss of cell surface microvilli; swollen mitochondria with disappearing cristae and vacuole-like structure; and

increased secondary lysosomes in alveolar macrophages. The TNF-α

the pathological morphology of lung injury was less severe in all three treatment groups. In addition

TGF-β and IL-1β and WBC count in BALF were decreased (P<0.01 or P<0.05)

and TFF3 mRNA expression in the lung tissue was significantly increased in the PLF group (P<0.01). Compared with the LF group

the IL-1β in BALF was significantly decreased P<0.05)

关键词

Radix Platycodoncleaning-heat and detoxificationChronic Obstructive Pulmonary Diseaseintestinal trefoil factor 3Fei-targeting

Keywords

Radix Platycodoncleaning-heat and detoxificationChronic Obstructive Pulmonary Diseaseintestinal trefoil factor 3Fei-targeting

references

Qing Dynasty·Huang GX. Seeking accuracy in the materia medica. 1st ed. Beijing: China Press of Traditional Chinese Medicine; 1999:104.

Nie HM, ed. Treatise on cold damage and clinical practice. 1st ed. Guangzhou: Guangzhou Science and Technology Press; 1993:586–587.

Li YH, Huang Y, Guo MZ, Zhong XG, Jia X, Wu YF, et al. Influence of Chinese herbal drugs combined with Radix Platycodi on pathomorphological changes in rats with COPD. J Beijing Univ Chin Med (Chin) 2008;31:819–822.

Li HM, Cui DJ, Tong X, Pang BS, Gao YB, Wang DW. Establishment of chronic obstructive pulmonary disease rat models by passive cigarette smoking and intratracheal instillation of lipopolysaccharide. Chin J Pathophysiol (Chin) 2002;18:808–812.

Huang Y, Guo MZ, Li LN, Zhong XG, Wu YF, LI YH. Effect of Radix Platycodi on mRNA expression of TFF3 in different tissues of rats with chronic obstructive pulmonary diseases. Chin J Basic Med Tradit Chin Med (Chin) 2009;15:69–71.

Newton R, Holden NS, Catley MC, Oyelusi W, Leigh R, Proud D, et al. Repression of inflammatory gene expression in human pulmonary epithelial cells by smallmolecule IkappaB kinase inhibitors. J Pharmacol Exp Ther 2007;321:734–742.

Hicks A, Monkarsh SP, Hoffman AF, Goodnow R Jr. Leukotriene B4 receptor antagonists as therapeutics for inflammatory disease: preclinical and clinical developments. Expert Opin Invest Drugs 2007;16:1909–1920.

Bailey WC, Tashkin DP. Pharmacologic therapy: novel approaches for chronic obstructive pulmonary disease. Proc Am Thorac Soc 2007;4:543–548.

Macklem PT. Therapeutic implications of the pathophysiology of COPD. Eur Respir J 2010;35:676–680.

Guidelines: executive summary: global strategy for the diagnosis, management, and prevention of COPD. December 2009(http://www.goldcopd.com/).

Chen N, Li H, Zhao LY, Liu JB, Huang Q, Wang TH, et al. Therapy for clearing heat and resolving phlegm in treatment of systemic inflammatory response syndrome in acute deterioration stage of chronic obstructive pulmonary disease: a randomized controlled trial. J Chin Integr Med (Chin) 2009;7:105–109.

Yu XQ, Li JS, Li L. Study on distribution regularity of TCM syndromes in chronic obstructive pulmonary disease patients. J Henan Univ Chin Med (Chin) 2003;18:44–46.

You BW, Zhou K. Experience of chronic obstructive pulmonary disease therapy for Huoxue Qingfei Decoction (活血清肺饮) combined with Western medicine: a repor of 25 cases. J Tradit Chin Med (Chin)2009;50:721–722.

Song LR, Hong X, Ding XL, Zang DY. Dictionary of modern Chinese medicine. 1st ed. Beijing: People’s Medical Publishing House; 2002:1652.

Wiede A, Jagla W, Welte T, Köhnlein T, Busk H, Hoffmann W. Localization of TFF3, a new mucus-associated peptide of the human respiratory tract. Am J Respir Crit Care Med 1999;159:1330–1335.

LeSimple P, van Seuningen I, Buisine MP, Copin MC, Hinz M, Hoffmann W, et al. Trefoil factor family 3 peptide promotes human airway epithelial ciliated cell differentiation. Am J Respir Cell Mol Biol 2007;36:296–303.

Thim L, May FE. Structure of mammalian trefoil factors and functional insights. Cell Mol Life Sci 2005;62:2956–2973.

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Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University

Department of Traditional Chinese Medicine, the 2nd Xiangya Hospital, Central South University

Department of Respiratory, Huaihe Hospital of Henan University

Department of Respiratory, Kaifeng Hospital of Traditional Chinese Medicine

Department of Respiratory, the Second People’s Hospital of Jiaozuo City

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