Effects of Jianpi Jiedu Recipe (健脾解毒方) on reversion of P-glycoprotein-mediated multidrug resistance through COX-2 pathway in colorectal cancer

Hua Sui; Hui-rong Zhu; Jie Wu; Alexander Yu Nikitin; Jian-feng Cai; Zhong-ze Fan; Qi Li

doi:10.1007/s11655-013-1452-4

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Effects of Jianpi Jiedu Recipe (健脾解毒方) on reversion of P-glycoprotein-mediated multidrug resistance through COX-2 pathway in colorectal cancer

Updated：2021-08-27

- Effects of Jianpi Jiedu Recipe (健脾解毒方) on reversion of P-glycoprotein-mediated multidrug resistance through COX-2 pathway in colorectal cancer
- Chinese Journal of Integrative Medicine Vol. 20, Issue 8, Pages: 610-617(2014)
- Affiliations：
  
  1. Department of Medical Oncology, Shuguang Hospital & Putuo Hospital, Shanghai University of Traditional Chinese Medicine,Shanghai,China
  2. Department of Molecular Oncology, H. Lee Moffitt Cancer Center and Research Institute,Tampa,USA
  3. Department of Biomedical Sciences, Cornell University, Ithaca,USA
  4. Department of Chemistry, University of South Florida,Tampa,USA
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No.81202812), Science and Technology Commission of Shanghai Municipality (No. 10ZR1427400), Program of Shanghai Municipal Education Commission (No. 09YZ132, 2011JW57), and Shanghai Municipal Health Bureau (No. 2011ZJ030, 20114Y013)
- DOI：10.1007/s11655-013-1452-4
  CLC：
- Published：2014，
  
  Published Online：1 April 2013，
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Sui, H., Zhu, Hr., Wu, J. et al. Effects of Jianpi Jiedu Recipe (健脾解毒方) on reversion of P-glycoprotein-mediated multidrug resistance through COX-2 pathway in colorectal cancer., Chin. J. Integr. Med. 20, 610–617 (2014). https://doi.org/10.1007/s11655-013-1452-4

Hua Sui, Hui-rong Zhu, Jie Wu, et al. Effects of Jianpi Jiedu Recipe (健脾解毒方) on reversion of P-glycoprotein-mediated multidrug resistance through COX-2 pathway in colorectal cancer. [J]. Chinese Journal of Integrative Medicine 20(8):610-617(2014)
Sui, H., Zhu, Hr., Wu, J. et al. Effects of Jianpi Jiedu Recipe (健脾解毒方) on reversion of P-glycoprotein-mediated multidrug resistance through COX-2 pathway in colorectal cancer., Chin. J. Integr. Med. 20, 610–617 (2014). https://doi.org/10.1007/s11655-013-1452-4 DOI：

Hua Sui, Hui-rong Zhu, Jie Wu, et al. Effects of Jianpi Jiedu Recipe (健脾解毒方) on reversion of P-glycoprotein-mediated multidrug resistance through COX-2 pathway in colorectal cancer. [J]. Chinese Journal of Integrative Medicine 20(8):610-617(2014) DOI： 10.1007/s11655-013-1452-4.

摘要

To evaluate the underlying mechanism of Jianpi Jiedu Recipe (健脾解毒方

JJR) in the reversion of multidrug resistance concerning colorectal cancer in vitro and in vivo. Mice were treated orally with JJR at a daily 4.25 g/(kg·day) or injected with vinblastine (VCR) 2.5 mg/(kg·day) for 3 weeks after having been inoculated with HCT8/V cells; tumor tissues were assayed by hematoxylin and eosin staining. Firstly

the effects of JJR on the expression of cyclooxygenase-2 (COX-2) were tested by real-time polymerase chain reaction (PCR) technique and COX-2 gene silenced by siRNA. Secondly

the variation of intracellular concentration of oxaliplatin (L-OHP) was evaluated by the inductively coupled plasma mass spectroscopy (ICPMS) in HCT8/V and its COX-2 siRNA cells; the concentration of JJR combined with chemotherapeutic drugs and the reverse effect of multidrug resistance (MDR) in HCT8/V cells was evaluated by the MTT assay. Thirdly

real-time quantitative PCR and Western blot analysis were used to detect the multidrug resistance gene 1 (MDR1) mRNA and P-gp expression. JJR had an inhibitory effect on the growth of tumors in vivo

and it

in combination with chemotherapeutic drugs

could reverse the drug-resistance of HCT8/V cells and increase the sensitivity of HCT8/V cells to VCR

DDP

5-Fu

and THP. ICP-MS results showed that JJR could increase the concentration of drugs in HCT8/V cells (P<0.01). Furthermore

it was shown that JJR could reverse drug resistance of colorectal cancer cells by decreasing MDR1 expression and P-gp level via downregulation of COX-2

which has been represented as one of the major mechanisms that contributes to the MDR phenotype (P<0.01). JJR reversed multidrug resistance and enhanced the sensitivity to chemotherapy

which could be attributed to the down-regulation of COX-2 in MDR1/P-gp-mediated MDR colorectal cancer after chemotherapy.

Abstract

To evaluate the underlying mechanism of Jianpi Jiedu Recipe (健脾解毒方

the effects of JJR on the expression of cyclooxygenase-2 (COX-2) were tested by real-time polymerase chain reaction (PCR) technique and COX-2 gene silenced by siRNA. Secondly

real-time quantitative PCR and Western blot analysis were used to detect the multidrug resistance gene 1 (MDR1) mRNA and P-gp expression. JJR had an inhibitory effect on the growth of tumors in vivo

and it

in combination with chemotherapeutic drugs

could reverse the drug-resistance of HCT8/V cells and increase the sensitivity of HCT8/V cells to VCR

DDP

5-Fu

and THP. ICP-MS results showed that JJR could increase the concentration of drugs in HCT8/V cells (P<0.01). Furthermore

it was shown that JJR could reverse drug resistance of colorectal cancer cells by decreasing MDR1 expression and P-gp level via downregulation of COX-2

which has been represented as one of the major mechanisms that contributes to the MDR phenotype (P<0.01). JJR reversed multidrug resistance and enhanced the sensitivity to chemotherapy

which could be attributed to the down-regulation of COX-2 in MDR1/P-gp-mediated MDR colorectal cancer after chemotherapy.

关键词

colorectal cancermultidrug resistanceP-glycoproteinmultidrug resistance genecyclooxygenase-2Jianpi Jiedu RecipeChinese Medicine

Keywords

colorectal cancermultidrug resistanceP-glycoproteinmultidrug resistance genecyclooxygenase-2Jianpi Jiedu RecipeChinese Medicine

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Department of Colorectal and Anal Surgery, the First Affiliated Hospital of Guiyang College of Traditional Chinese Medicine

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