Effects of herbal compound 861 on collagen synthesis and degradation in rat mesangial cells exposed to high glucose

Qing Ma; Li Zhang; Lan Yao; Hai-ping Chen; Bao-en Wang

doi:10.1007/s11655-014-1741-6

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Effects of herbal compound 861 on collagen synthesis and degradation in rat mesangial cells exposed to high glucose

Updated：2021-08-27

- Effects of herbal compound 861 on collagen synthesis and degradation in rat mesangial cells exposed to high glucose
- Chinese Journal of Integrative Medicine Vol. 20, Issue 3, Pages: 209-215(2014)
- Affiliations：
  
  Department of Gerontology, Beijing Friendship Hospital, Capital Medical University,Beijing,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-014-1741-6
  CLC：
- Published：2014，
  
  Published Online：4 March 2014，
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Ma, Q., Zhang, L., Yao, L. et al. Effects of herbal compound 861 on collagen synthesis and degradation in rat mesangial cells exposed to high glucose., Chin. J. Integr. Med. 20, 209–215 (2014). https://doi.org/10.1007/s11655-014-1741-6

Qing Ma, Li Zhang, Lan Yao, et al. Effects of herbal compound 861 on collagen synthesis and degradation in rat mesangial cells exposed to high glucose. [J]. Chinese Journal of Integrative Medicine 20(3):209-215(2014)
Ma, Q., Zhang, L., Yao, L. et al. Effects of herbal compound 861 on collagen synthesis and degradation in rat mesangial cells exposed to high glucose., Chin. J. Integr. Med. 20, 209–215 (2014). https://doi.org/10.1007/s11655-014-1741-6 DOI：

Qing Ma, Li Zhang, Lan Yao, et al. Effects of herbal compound 861 on collagen synthesis and degradation in rat mesangial cells exposed to high glucose. [J]. Chinese Journal of Integrative Medicine 20(3):209-215(2014) DOI： 10.1007/s11655-014-1741-6.

摘要

To investigate the effects of Herbal Compound 861 (Cpd 861) on collagen synthesis and degradation in rat mesangial cells exposed to high glucose. The third to fifth passage of rat mesangial cells were exposed to high glucose and Cpd 861 at a concentration of 0.25–4.00 g/L for 24

48 and 72 h

respectively. Benazepril (10−7–10−3 mmol/L) was selected as positive control. The methyl thiazolyl tetrazolium colorimetric assay was used to evaluate the effect of Cpd 861 on cell proliferation. After incubation with Cpd 861 at a concentration of 2.00 g/L for 48 h

the protein secretions of collagen type IV

matrix metallopeptidase 9 (MMP-9)

tissue inhibitor of metalloproteinase-1 (TIMP-1)

transforming growth factor beta 1 (TGF-β1)

and hepatocyte growth factor (HGF) were detected by enzyme-linked immunosorbent assay method. And rat mesangial cells were harvested to determine MMP-9

TIMP-1

TGF-β1 and HGF mRNA expression by reverse transcription polymerase chain reaction. Cpd 861 inhibited cell proliferation induced by high glucose in a dose- and time-dependent manner. Compared with high glucose

collagen type IV production was decreased significantly by Cpd 861 (P<0.01). Cpd 861 increased the protein secretions and mRNA expressions of MMP-9 and HGF

whereas the protein secretions and mRNA expressions of TIMP-1 and TGF-β1 were reduced markedly (P<0.05). The ratio of MMP-9 to TIMP-1 was enhanced by Cpd 861 significantly. There was no significant difference in all above-mentioned effects between Cpd 861 (2.00 g/L) and benazepril (10−5 mmol/L). The anti-glomerulosclerosis mechanisms of Cpd 861 were partly attributed to its effects of inhibiting mesangial cell proliferation

decreasing collagen synthesis and enhancing collagen degradation.

Abstract

48 and 72 h

the protein secretions of collagen type IV

matrix metallopeptidase 9 (MMP-9)

tissue inhibitor of metalloproteinase-1 (TIMP-1)

transforming growth factor beta 1 (TGF-β1)

and hepatocyte growth factor (HGF) were detected by enzyme-linked immunosorbent assay method. And rat mesangial cells were harvested to determine MMP-9

TIMP-1

collagen type IV production was decreased significantly by Cpd 861 (P<0.01). Cpd 861 increased the protein secretions and mRNA expressions of MMP-9 and HGF

decreasing collagen synthesis and enhancing collagen degradation.

关键词

Herbal Compound 861mesangial cellscollagen type IVmatrix metalloproteinasecytokine

Keywords

Herbal Compound 861mesangial cellscollagen type IVmatrix metalloproteinasecytokine

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