Shen-Fu Injection (参附注射液) alleviates post-resuscitation myocardial dysfunction by up-regulating expression of sarcoplasmic reticulum Ca2+-ATPase

Zhi-jun Guo; Cai-jun Wu; Chun-sheng Li

doi:10.1007/s11655-015-2156-8

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Shen-Fu Injection (参附注射液) alleviates post-resuscitation myocardial dysfunction by up-regulating expression of sarcoplasmic reticulum Ca2+-ATPase

Updated：2021-08-27

- Shen-Fu Injection (参附注射液) alleviates post-resuscitation myocardial dysfunction by up-regulating expression of sarcoplasmic reticulum Ca2+-ATPase
- Chinese Journal of Integrative Medicine Vol. 22, Issue 7, Pages: 503-509(2016)
- Affiliations：
  
  1. Intensive Care Unit, Shandong Cancer Hospital and Institute,Jinan,China
  2. Department of Emergency, Beijing Chaoyang Hospital, Capital Medical University,Beijing,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-015-2156-8
  CLC：
- Published：2016，
  
  Published Online：11 August 2015，
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Guo, Zj., Wu, Cj. & Li, Cs. Shen-Fu Injection (参附注射液) alleviates post-resuscitation myocardial dysfunction by up-regulating expression of sarcoplasmic reticulum Ca2+-ATPase., Chin. J. Integr. Med. 22, 503–509 (2016). https://doi.org/10.1007/s11655-015-2156-8

Zhi-jun Guo, Cai-jun Wu, Chun-sheng Li. Shen-Fu Injection (参附注射液) alleviates post-resuscitation myocardial dysfunction by up-regulating expression of sarcoplasmic reticulum Ca2+-ATPase. [J]. Chinese Journal of Integrative Medicine 22(7):503-509(2016)
Guo, Zj., Wu, Cj. & Li, Cs. Shen-Fu Injection (参附注射液) alleviates post-resuscitation myocardial dysfunction by up-regulating expression of sarcoplasmic reticulum Ca2+-ATPase., Chin. J. Integr. Med. 22, 503–509 (2016). https://doi.org/10.1007/s11655-015-2156-8 DOI：

Zhi-jun Guo, Cai-jun Wu, Chun-sheng Li. Shen-Fu Injection (参附注射液) alleviates post-resuscitation myocardial dysfunction by up-regulating expression of sarcoplasmic reticulum Ca2+-ATPase. [J]. Chinese Journal of Integrative Medicine 22(7):503-509(2016) DOI： 10.1007/s11655-015-2156-8.

摘要

To compare the effect of Shen-Fu Injection (SFI) and epinephrine on the expression of sarcoplasmic reticulum Ca2+ ATPase 2a (SERCA2a) in a pig model with post-resuscitation myocardial dysfunction. Ventricular fibrillation (VF) was electrically induced in Wu-zhi-shan miniature pigs. After 8 min of untreated VF and 2 min of cardiopulmonary resuscitation (CPR)

all animals were randomly administered a bolus injection of saline placebo (SA group

n=10)

SFI (0.8 mg/kg

SFI group

n=10) or epinephrine (20 μg/kg

EPI group

n=10). After 4 min of CPR

a 100-J shock was delivered. If the defibrillation attempt failed to attain restoration of spontaneous circulation (ROSC)

manual chest compressions were rapidly resumed for a further 2 min followed by a second defibrillation attempt. Hemodynamic variables were recorded

and plasma concentrations of catecholamines were measured. Adenylate cyclase (AC)

cyclic adenosine monophosphate (cAMP) and the expressions of β1-adrenoceptor (AR) and SERCA 2a were determined. Cardiac output

left ventricular dp/dtmax and negative dp/dtmax were significantly higher in the SFI group than in the SA and EPI groups at 4 and 6 h after ROSC. The expression of β1-AR and SERCA2a at 24 h after ROSC were significantly higher in the SFI group than in the SA and EPI groups (P<0.05 or P<0.01). The administration of epinephrine during CPR decreased the expression of SERCA2a and aggravated postresuscitation myocardial function (P<0.01). SFI attenuated post-resuscitation myocardial dysfunction

and the mechanism might be related to the up-regulation of SERCA2a expression.

Abstract

all animals were randomly administered a bolus injection of saline placebo (SA group

n=10)

SFI (0.8 mg/kg

SFI group

n=10) or epinephrine (20 μg/kg

EPI group

n=10). After 4 min of CPR

a 100-J shock was delivered. If the defibrillation attempt failed to attain restoration of spontaneous circulation (ROSC)

manual chest compressions were rapidly resumed for a further 2 min followed by a second defibrillation attempt. Hemodynamic variables were recorded

and plasma concentrations of catecholamines were measured. Adenylate cyclase (AC)

cyclic adenosine monophosphate (cAMP) and the expressions of β1-adrenoceptor (AR) and SERCA 2a were determined. Cardiac output

and the mechanism might be related to the up-regulation of SERCA2a expression.

关键词

epinephrineShen-fu Injectionsarcoplasmic reticulum Ca2+ ATPase 2acardiac arrestcardiopulmonary resuscitationChinese Medicine

Keywords

epinephrineShen-fu Injectionsarcoplasmic reticulum Ca2+ ATPase 2acardiac arrestcardiopulmonary resuscitationChinese Medicine

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Department of Emergency Medicine, Beijing Chaoyang Hospital, Capital Medical University

Beijing Key Laboratory of Cardiopulmonary Cerebral Resuscitation

Department of Hyperbaric Oxygen, Beijing Chao-Yang Hospital, Capital Medical University

Department of Emergency, Beijing Chao-Yang Hospital, Capital Medical University

Emergency Department of Beijing Chaoyang Hospital, Capital Medical University

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