Effect of ligustrazine nanoparticles nano spray on transforming growth factor-β/Smad signal pathway of rat peritoneal mesothelial cells induced by tumor necrosis factor-α

Shuai Yan; Lan Yang; Yin-zi Yue; Wen-lin Li; Li Zeng; Jin Yue; Chun-qin Mao

doi:10.1007/s11655-015-2180-8

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Effect of ligustrazine nanoparticles nano spray on transforming growth factor-β/Smad signal pathway of rat peritoneal mesothelial cells induced by tumor necrosis factor-α

Updated：2021-08-27

- Effect of ligustrazine nanoparticles nano spray on transforming growth factor-β/Smad signal pathway of rat peritoneal mesothelial cells induced by tumor necrosis factor-α
- Chinese Journal of Integrative Medicine Vol. 22, Issue 8, Pages: 629-634(2016)
- Affiliations：
  
  1. Suzhou Hospital of Traditional Chinese Medicine,Jiangsu Province,Suzhou,China
  2. First Clinical Medical College of Nanjing University of Chinese Medicine,Nanjing,China
  3. Library of Nanjing University of Chinese Medicine,Nanjing,China
  4. Yancheng Hospital of Traditional Chinese Medicine,Jiangsu Province,Yancheng,China
  5. College of Pharmacy of Nanjing University of Chinese Medicine,Nanjing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81373843) and College Graduate Student Innovative Research Fund of Jiangsu Province, China (No. CXZZ13-0613) and Administration of Traditional Chinese Medicine Science and Technology Project of Jiangsu Province, China (No. LZ13006)
- DOI：10.1007/s11655-015-2180-8
  CLC：
- Published：2016，
  
  Published Online：30 September 2015，
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Yan, S., Yang, L., Yue, Yz. et al. Effect of ligustrazine nanoparticles nano spray on transforming growth factor-β/Smad signal pathway of rat peritoneal mesothelial cells induced by tumor necrosis factor-α., Chin. J. Integr. Med. 22, 629–634 (2016). https://doi.org/10.1007/s11655-015-2180-8

Shuai Yan, Lan Yang, Yin-zi Yue, et al. Effect of ligustrazine nanoparticles nano spray on transforming growth factor-β/Smad signal pathway of rat peritoneal mesothelial cells induced by tumor necrosis factor-α. [J]. Chinese Journal of Integrative Medicine 22(8):629-634(2016)
Yan, S., Yang, L., Yue, Yz. et al. Effect of ligustrazine nanoparticles nano spray on transforming growth factor-β/Smad signal pathway of rat peritoneal mesothelial cells induced by tumor necrosis factor-α., Chin. J. Integr. Med. 22, 629–634 (2016). https://doi.org/10.1007/s11655-015-2180-8 DOI：

Shuai Yan, Lan Yang, Yin-zi Yue, et al. Effect of ligustrazine nanoparticles nano spray on transforming growth factor-β/Smad signal pathway of rat peritoneal mesothelial cells induced by tumor necrosis factor-α. [J]. Chinese Journal of Integrative Medicine 22(8):629-634(2016) DOI： 10.1007/s11655-015-2180-8.

摘要

To study the effect of ligustrazine nanoparticles nano spray (LNNS) on transforming growth factor β (TGF-β)/Smad signal protein of rat peritoneal mesothelial cells (RPMC) induced by tumor necrosis factor α (TNF-α)

and the anti-adhesion mechanism of LNNS in the abdominal cavity. The primary culture and subculture of rat peritoneal mesothelial cells (RPMC) was processed by trypsin digestion method in vitro. The third generation was identifified for experiment and divided into 5 groups: a blank group: RPMC without treatment; a control group: RPMC stimulated with TNF-α; RPMC treated by a low-dosage LNNS group (2.5 mg/L); RPMC treated by a medium-dosage LNNS group (5 mg/L); and RPMC treated by a high-dosage LNNS group (10 mg/L). Reverse transcription-polymerase chain reaction was applied to test the expression of fifibronectin

collagen I (COL-I)

TGF-β mRNA

and Western blot method to test the Smad protein 7 expression of RPMC. Compared with the blank group

a signifificant elevation in fifibronectin (FN)

COL-I and TGF-β mRNA expression of RPMC were observed in the control group (P<0.05). Compared with the control group

LNNS suppressed the expressions of FN

COL-I and TGF-β mRNA in a concentrationdependent manner (P<0.05). The expression of Smad7 protein of RPMC was down-regulated by TNF-α stimulation

and up-regulated with the increase of LNNS dose (P<0.05). TNF-α may induce changes in RPMC’s viability

leading to peritoneal injury. LNNS could reverse the induction of fifibrosis related cytokine FN

COL-I and TGF-β

up-regulating the expression of Smad7 by TNF-α in RPMC

thus attenuate peritoneal injury by repairing mesothelial cells.

Abstract

collagen I (COL-I)

TGF-β mRNA

and Western blot method to test the Smad protein 7 expression of RPMC. Compared with the blank group

a signifificant elevation in fifibronectin (FN)

COL-I and TGF-β mRNA expression of RPMC were observed in the control group (P<0.05). Compared with the control group

LNNS suppressed the expressions of FN

COL-I and TGF-β mRNA in a concentrationdependent manner (P<0.05). The expression of Smad7 protein of RPMC was down-regulated by TNF-α stimulation

and up-regulated with the increase of LNNS dose (P<0.05). TNF-α may induce changes in RPMC’s viability

leading to peritoneal injury. LNNS could reverse the induction of fifibrosis related cytokine FN

COL-I and TGF-β

up-regulating the expression of Smad7 by TNF-α in RPMC

thus attenuate peritoneal injury by repairing mesothelial cells.

关键词

peritoneal mesothelial cellsLigustrazineexperimental researchtransforming growth factor β/Smad

Keywords

peritoneal mesothelial cellsLigustrazineexperimental researchtransforming growth factor β/Smad

references

Parker MC, Wilson MS, van Goor H, Moran BJ, Jeekel J, Duron JJ, et al. Adhesions and colorectal surgery—call for action. Colorectal Dis 2007;9:66–72.

Cheong YC, Laird SM, Li TC, Shelton JB, Ledger WL, Cooke ID. Peritoneal healing and adhesion formation/ reformation. Hum Reprod Update 2001;7:556–566.

Kössi J, Salminen P, Rantala A, Laato M. Population-based study of the surgical workload and economic impact of bowel obstruction caused by postoperative adhesions. Br J Surg 2003;90:1441–1444.

Schnuriger B, Barmparas G, Branco BC, Lustenberger T, Inaba K, Demetriades D. Prevention of postoperative peritoneal adhesions: a review of the literature. Am J Surg 2011;201:111–121.

Brüggmann D, Tchartchian G, Wallwiener M, Münstedt K, Tinneberg HR, Hackethal A. Intra-abdominal adhesions: definition, origin, significance in surgical practice, and treatment options. Dtsch Arztebl Int 2010;107:769–775.

Ergul E, Korukluoglu B. Peritoneal adhesions: facing the enemy. Int J Surg 2008;6:253–260.

Liakakos T, Thomakos N, Fine PM, Dervenis C, Young RL. Peritoneal adhesions: etiology, pathophysiology, and clinical significance. Recent advances in prevention and management. Dig Surg 2001;18:260–273.

Ward BC, Panitch A. Abdominal adhesions: current and novel therapies. J Surg Res 2011;165:91–111.

Ambler DR, Fletcher NM, Diamond MP, Saed GM. Effects of hypoxia on the expression of inflammatory markers IL-6 and TNF-a in human normal peritoneal and adhesion fibroblasts. Syst Biol Reprod Med 2012;58:324–329.

Corona R, Verguts J, Schonman R, Binda MM, Mailova K, Koninckx PR. Postoperative inflammation in the abdominal cavity increases adhesion formation in a laparoscopic mouse model. Fertil Steril 2011;95:1224–1228.

Pismensky SV, Kalzhanov ZR, Eliseeva MY, Kosmas IP, Mynbaev OA. Severe inflammatory reaction induced by peritoneal trauma is the key driving mechanism of postoperative adhesion formation. BMC Surg 2011;11:30.

ten Broek RP, Wilbers J, van Goor H. Electrocautery causes more ischemic peritoneal tissue damage than ultrasonic dissection. Surg Endosc 2011;25:1827–1834.

Thompson J. Pathogenesis and prevention of adhesion formation. Dig Surg 1998;15:153–154.

Holmdahl L, Ivarsson ML. The role of cytokines, coagulation, and fibrinolysis in peritoneal tissue repair. Eur J Surg 1999;165:1012–1019.

Falk P, Ma C, Chegini N, Holmdahl L. Differential regulation of mesothelial cell fibrinolysis by transforming growth factor beta 1. Scand J Clin Lab Invest 2000;60:439–447.

Liu Y. Epithelial to mesenchymal transition in renal fibrogenesis: pathologic significance, molecular mechanism, and therapeutic intervention. J Am Soc Nephrol 2004;15:1–12.

Guo H, Leung JC, Cheung JS, Chan LY, Wu EX, Lai KN. Non-viral Smad7 gene delivery and attenuation of postoperative peritoneal adhesion in an experimental model. Br J Surg 2009;96:1323–1335.

Mao CQ, Zeng L, Lu TL, Wang XW, Li ZY, Su T. Effects of Ligustrazine nano spray on celiac adhesion related factors and fibrinolysis system in rats. Chin Pharmacol Bull 2012;28:118–123.

Li ZY, Mao CQ, Zeng L, Lu TL. Determination of entrapment efficiency of tetramethylpyrazine polylactic aci nanoparticles. J Nangjing Univ Tradit Chin Med (Chin) 2011;27:77–79.

Zeng L, Mao CQ, Lu TL, Qian L, Li ZY. Su T. Preparation technology and quality assay of ligustrazine poly lactic acid nanoparticles. Chin Tradit Patent Med (Chin) 2013;35:261–263.

Zeng L, Mao CQ, Lu TL, Qian L, Li ZY, Su T. Culture and identification of peritoneal mesothelium cells in rats. J Nangjing Univ Tradit Chin Med (Chin) 2012;28:334–336.

Coccolini F, Ansaloni L, Manfredi R, Campanati L, Poiasina E, Bertoli P, et al. Peritoneal adhesion index (PAI): proposal of a score for the “ignored iceberg” of medicine and surgery. World J Emerg Surg 2013;8:6.

Verrecchia F, Mauviel A. Transforming growth factor-beta signaling through the Smad pathway: role in extracellular matrix gene expressionand regulation. J Invest Dermatol 2002;118:211–215.

Zhang SK, Cui NQ, Zhuo YZ, Li DH, Liu JH. Modified Xiaochaihu Decoction prevents the progreßsion of chronic pancreatitis in rats possibly by inhibitingtransforming growth factor-1/Sma-and mad-related proteins signaling pathway. Chin J Integr Med 2013;19:935–939..

Maciver AH, McCall M, James Shapiro AM. Intra-abdominal adhesions: cellular mechanisms and strategies for prevention. Int J Surg 2011;9:589–594.

Bai JR, Wu XZ. The mechanism and prevention of postoperative peritoneal adhesion. Chin J Surg Integr Tradit West Med (Chin) 2011;17:332–336.

Che XW, Zhang Y, Wang H, Wang W. Effect of ligustrazine injection on levels of interleukin-4 and interferon-gamma in patients with bronchial asthma. Chin J Integr Med 2008;14:217–220.

Mark C, Ester P, Anita R. Role of transforming growth factor-ß signaling in cancer. J Natl Cancer Inst 2000;92:1388–1402.

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Fujian University of Traditional Chinese Medicine

Department of Endocrinology, the People’s Hospital Affiliated to Fujian Traditional Chinese Medicine University

Department of Tumor and Hematology, Dongzhimen Hospital of Beijing University of Chinese Medicine

. for Research on the Theory of Qi-Blood of Integrated Medicine, Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine, Beijing

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