Influence of Yanyankang Powder (眼炎康散) on Th1/Th2 in rats with experimental autoimmune uveitis
Back to article page
|Updated:2021-08-27
|
Influence of Yanyankang Powder (眼炎康散) on Th1/Th2 in rats with experimental autoimmune uveitis
Chinese Journal of Integrative MedicineVol. 22, Issue 3, Pages: 214-218(2016)
Affiliations:
1. Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine,Jinan,China
2. Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan,China
3. Eye Institute of Shandong University of Traditional Chinese Medicine,Jinan,China
Author bio:
Funds:
Supported by the National Natural Science Foundation of China (No. 81072961, 81100658 and 81373826) and the National Science Foundation for Post-doctoral Scientists of China (No. 2012M510068)
Tian, Qm., Bi, Hs., Cui, Y. et al. Influence of Yanyankang Powder (眼炎康散) on Th1/Th2 in rats with experimental autoimmune uveitis., Chin. J. Integr. Med. 22, 214–218 (2016). https://doi.org/10.1007/s11655-015-2182-6
Qing-mei Tian, Hong-sheng Bi, Yan Cui, et al. Influence of Yanyankang Powder (眼炎康散) on Th1/Th2 in rats with experimental autoimmune uveitis. [J]. Chinese Journal of Integrative Medicine 22(3):214-218(2016)
Tian, Qm., Bi, Hs., Cui, Y. et al. Influence of Yanyankang Powder (眼炎康散) on Th1/Th2 in rats with experimental autoimmune uveitis., Chin. J. Integr. Med. 22, 214–218 (2016). https://doi.org/10.1007/s11655-015-2182-6DOI:
Qing-mei Tian, Hong-sheng Bi, Yan Cui, et al. Influence of Yanyankang Powder (眼炎康散) on Th1/Th2 in rats with experimental autoimmune uveitis. [J]. Chinese Journal of Integrative Medicine 22(3):214-218(2016) DOI: 10.1007/s11655-015-2182-6.
Influence of Yanyankang Powder (眼炎康散) on Th1/Th2 in rats with experimental autoimmune uveitis
摘要
To study the influence of Yanyankang Powder (眼炎康散) on Th1/Th2 in rats with experimental autoimmune uveitis (EAU). The EAU models were induced in Lewis rats by immunization with interphotoreceptor retinoid binding protein (IRBP) 1177–1191 in complete Freund's adjuvant. The rats were randomly divided into 3 groups: a model control group
a Yanyankang group
and a prednisone group
9 rats in each group. The model control group was intervened with saline solution by gavage. The Yanyankang group was intervened with Yanyankang Powder 4 g/(kg day) by gavage. The prednisone group were intervened with prednisone acetate tablets 5 mg/(kg d) by gavage. All groups were intervened after immunization once every 2 days for 18 days and monitored by slit-lamp biomicroscopy daily until day 18. The levels of gamma interferon (INF-γ) and interleukin-10 (IL-10) in the supernatants of T cells were determined by enzyme-linked immunosorbent assay. Polymerase chain reaction (PCR) technology was used for measuring Th1 and Th2 related cytokine mRNA expressions. Slighter intraocular inflammation was found in the Yanyankang group and the prednisone group than the control group. The levels of the IFN-γand IL-10 in the supernatants of the spleen lymph node cells were 382.33±6.30
155.87±4.46 μg/L in the Yanyankang group and 270.93±7.76
265.32±11.88 μg/L in the prednisone group. Both had significant differences compared with the control group (941.53±8.59
20.67±4.65 μg/L; =0.01). The PCR results showed the same tendency. Yanyankang Powder showed favorable effects in the rats with EAU by influencing the function of Th1 and Th2 cells.
Abstract
To study the influence of Yanyankang Powder (眼炎康散) on Th1/Th2 in rats with experimental autoimmune uveitis (EAU). The EAU models were induced in Lewis rats by immunization with interphotoreceptor retinoid binding protein (IRBP) 1177–1191 in complete Freund's adjuvant. The rats were randomly divided into 3 groups: a model control group
a Yanyankang group
and a prednisone group
9 rats in each group. The model control group was intervened with saline solution by gavage. The Yanyankang group was intervened with Yanyankang Powder 4 g/(kg day) by gavage. The prednisone group were intervened with prednisone acetate tablets 5 mg/(kg d) by gavage. All groups were intervened after immunization once every 2 days for 18 days and monitored by slit-lamp biomicroscopy daily until day 18. The levels of gamma interferon (INF-γ) and interleukin-10 (IL-10) in the supernatants of T cells were determined by enzyme-linked immunosorbent assay. Polymerase chain reaction (PCR) technology was used for measuring Th1 and Th2 related cytokine mRNA expressions. Slighter intraocular inflammation was found in the Yanyankang group and the prednisone group than the control group. The levels of the IFN-γand IL-10 in the supernatants of the spleen lymph node cells were 382.33±6.30
155.87±4.46 μg/L in the Yanyankang group and 270.93±7.76
265.32±11.88 μg/L in the prednisone group. Both had significant differences compared with the control group (941.53±8.59
20.67±4.65 μg/L; =0.01). The PCR results showed the same tendency. Yanyankang Powder showed favorable effects in the rats with EAU by influencing the function of Th1 and Th2 cells.
关键词
Yanyankang powderexperimental autoimmune uveitisgamma interferoninterleukin-10Lewis ratChinese Medicine
Keywords
Yanyankang powderexperimental autoimmune uveitisgamma interferoninterleukin-10Lewis ratChinese Medicine
references
Whitcup SM, Nussenblatt RB. Immunologic mechanisms of uveitis. New targets for immunomodulation. Arch Ophthalmol 1997;115:520–525.
Rothova A, Suttorp VS, Frits T, Kijlstra A. Causes and frequency of blindness in patients with intraocular inflammatory disease. Br J Ophthalmol 1996;80:332–336.
Huang M, Hao YL, Chen MR, Xie XF. Preparation and quality control of Yanyankang San. Chin J Hospit Pharm (Chin) 2010;30:1714–1716.
The Ministry of Science and Technology of the People's Republic of China. Guidance suggestions for the care and use of laboratory animals. 2006-9-30.
Jia XZ, Hu MH, Wang CH, Wang CY, Zhang FY, Han QL, et al. Coordinated gene expression of Th17- and Tregassociated molecules correlated with resolution of the monophasic experimental autoimmune uveitis. Molecular Vision 2011;17:1493–1507.
Agarwal RK, Caspi RR. Rodent models of experimental autoimmune uveitis. Methods Mol Med 2004;102:395–419.
Aibara M, Lindsey JD, Weinreb RN. Reduction of intraocular pressure in mouse eyes treated with latanoprost. Invest Ophthalmol Vis Sci 2002;43;146–150.
Caspi RR. Th1 and Th2 responses in pathogenesis and regulation of experimental autoimmune uveoretinitis. Int Rev Immunol 2002;21:197–208.
Crane IJ, Xu HP, Wallace C, Manivannan A, Mack M, Liversidge J, et al. Involvement of CCR5 in the passage of Th1-type cells across the blood-retina barrier in experimental autoimmune uveitis. J Leukoc Biol 2006;79:435–443.
Tarrant TK, Silver PB, Chan CC, Wiggert B, Caspi RR. Endogenous IL-12 is required for induction and expression of experimental autoimmune uveitis. Immunol 1998;161:122–127.
Sun B, Sun SH, Chan CC, Wiggert C, Caspi RR. Autoimmunity to a pathogenic retinal antigen begins as a balanced cytokine response that polarizes towards type 1 in a disease-susceptible and towards type 2 in a diseaseresistant genotype. Int Immunol 1999;11:1307–1312.
Mosmann TR. Properties and functions of interleukin-10. Adv Immunol 1994;56:1–26.
Cuzzocrea S, Mazzon E, Dugo L, Serraino I, Paola RD, Genovese T, et al. Absence of endogeneous interleukin-10 enhances the evolution of acute lung injury. Eur Cytokine Net 2002;13:285–297.
Lee YS, Ahjoku AO, Yu CR, Egwuagu CE. Retinal cells suppress intraocular inflammation (uveitis) through production of interleukin-27 and interleukin-10. Immunology 2011;132:492–502.
Rizzo LV, Xu H, Chan CC, Wiggert B, Caspi RR. IL-10 has a protective role in experimental autoimmune uveoretinitis. Int Immunol 1998;10:807–814.
Future view and development of immunology: Exploring the immunology based on Chinese medicine and culture
Cost-effectiveness analysis of combined Chinese medicine and Western medicine for ischemic stroke patients
Objective tongue inspection on 142 liver cancer patients with damp-heat syndrome
Effect of Ermiao Recipe (二妙方) with medicinal guide Angelicae Pubescentis Radix on promoting the homing of bone marrow stem cells to treat cartilage damage in osteoarthritis rats
Effects of Jianpi Jiedu Recipe (健脾解毒方) on reversion of P-glycoprotein-mediated multidrug resistance through COX-2 pathway in colorectal cancer
Related Author
No data
Related Institution
Georg-August-Universität Göttingen, Göttingen
The Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics’ Ministry of Public Health
Medical Informatics Center, Peking University
Department of Hospital Management, Peking University Health Science Center, Peking University
Neurology Department of Peking University Third Hospital, Peking University