Bushen Qiangji Granule (补肾强脊颗粒) medicated serum inhibits osteogenic differentiation of fibroblasts in ankylosing spondylitis by inhibiting the BMP/Smads signal pathway in vitro

Hong-xiao Liu; Nan Jiang; Hui-ying Liang; Ying-yan Zhou; Xing-hua Feng; Xiao-yan Feng; He-qiu Zhang; Zhi-kui Wu; Quan Jiang; Jiao Fu; Xiao-juan Ma; Peng Chen

doi:10.1007/s11655-016-2268-9

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Bushen Qiangji Granule (补肾强脊颗粒) medicated serum inhibits osteogenic differentiation of fibroblasts in ankylosing spondylitis by inhibiting the BMP/Smads signal pathway in vitro

Updated：2021-08-27

- Bushen Qiangji Granule (补肾强脊颗粒) medicated serum inhibits osteogenic differentiation of fibroblasts in ankylosing spondylitis by inhibiting the BMP/Smads signal pathway in vitro
- Chinese Journal of Integrative Medicine Vol. 22, Issue 11, Pages: 817-822(2016)
- Affiliations：
  
  1. Department of Rheumatology, Guang’anmen Hospital, China Academy of Chinese Medical Sciences,Beijing,China
  2. Department of Vaccine Engineering, Beijing Institute of Basic Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30472277 and No. 30801507), Beijing Science and Technology Nova Program (No. 713220 )
- DOI：10.1007/s11655-016-2268-9
  CLC：
- Published：2016，
  
  Published Online：26 October 2016，
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Liu, Hx., Jiang, N., Liang, Hy. et al. Bushen Qiangji Granule (补肾强脊颗粒) medicated serum inhibits osteogenic differentiation of fibroblasts in ankylosing spondylitis by inhibiting the BMP/Smads signal pathway in vitro, ., Chin. J. Integr. Med. 22, 817–822 (2016). https://doi.org/10.1007/s11655-016-2268-9

Hong-xiao Liu, Nan Jiang, Hui-ying Liang, et al. Bushen Qiangji Granule (补肾强脊颗粒) medicated serum inhibits osteogenic differentiation of fibroblasts in ankylosing spondylitis by inhibiting the BMP/Smads signal pathway in vitro. [J]. Chinese Journal of Integrative Medicine 22(11):817-822(2016)
Liu, Hx., Jiang, N., Liang, Hy. et al. Bushen Qiangji Granule (补肾强脊颗粒) medicated serum inhibits osteogenic differentiation of fibroblasts in ankylosing spondylitis by inhibiting the BMP/Smads signal pathway in vitro, ., Chin. J. Integr. Med. 22, 817–822 (2016). https://doi.org/10.1007/s11655-016-2268-9 DOI：

Hong-xiao Liu, Nan Jiang, Hui-ying Liang, et al. Bushen Qiangji Granule (补肾强脊颗粒) medicated serum inhibits osteogenic differentiation of fibroblasts in ankylosing spondylitis by inhibiting the BMP/Smads signal pathway in vitro. [J]. Chinese Journal of Integrative Medicine 22(11):817-822(2016) DOI： 10.1007/s11655-016-2268-9.

摘要

To explore the mechanism of Bushen Qiangji Granule (补肾强脊颗粒

BSQJ) in restraining the osteogenic differentiation of ankylosing spondylitis (AS) fifibroblasts. Hip joint capsules were obtained from AS patients (n=10) receiving total hip replacement and healthy hip joint capsules from patients with hip fracture (n=10) receiving surgery as a control. Finite fifibroblast lines were established from these tissue samples to observe the effect of BSQJ on suppressing osteogenic differentiation of fifibroblasts. The expression of osteogenic marker gene corebinding factor a1 (Cbfa1) and Smad family proteins were examined by Western blot and real-time quantitative polymerase chain reaction (qPCR). The mRNA expression level of Cbfa1 was significantly higher in AS fibroblasts than that in normal fibroblasts and the expression of pSmad1

pSmad5

Smad4 and Cbfa1 in AS fibroblasts was also higher

demonstrating the activation of the BMP/Smads signal pathway in AS fifibroblasts. BSQJ-medicated serum not only restrained the mRNA and protein expression levels of Cbfa1 and inhibited protein expression level of Smad4 but also decreased the expression quantities of pSmad1 and pSmad5. BSQJ can inhibit osteogenic differentiation of AS fifibroblasts in vitro by suppressing the activation of the BMP/Smads signal pathway. This may be the important molecular mechanism of BSQJ in regulating AS ossifification.

Abstract

To explore the mechanism of Bushen Qiangji Granule (补肾强脊颗粒

pSmad5

Smad4 and Cbfa1 in AS fibroblasts was also higher

关键词

Bushen Qiangji Granule medicated serumfifibroblastsankylosing spondylitisossifification

Keywords

Bushen Qiangji Granule medicated serumfifibroblastsankylosing spondylitisossifification

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Department of Rheumatology, Chinese PLA General Hospital

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the 208th Hospital of PLA

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