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Study on the effects of Guiqi Oral Liquid in promoting recovery of hematopoiesis in acute irradiation injured mice
Study on the effects of Guiqi Oral Liquid in promoting recovery of hematopoiesis in acute irradiation injured mice
- 中国结合医学杂志（英文版） 2005年11卷第4期 页码：283-286
- Affiliations：
Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong Science and Technology University,Wuhan
- Author bio：
- Funds：Supported by the National Natural Science Foundation of China (No. 39870926)
DOI：10.1007/BF02835790
中图分类号：纸质出版日期：2005，
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Miao, Z., Wen-li, L., Han-ying, S. et al. Study on the effects of Guiqi Oral Liquid in promoting recovery of hematopoiesis in acute irradiation injured mice., Chin. J. Integr. Med. 11, 283–286 (2005). https://doi.org/10.1007/BF02835790
Zheng Miao, Liu Wen-li, Sun Han-ying, et al. Study on the effects of Guiqi Oral Liquid in promoting recovery of hematopoiesis in acute irradiation injured mice[J]. Chinese Journal of Integrative Medicine, 2005,11(4):283-286.
Miao, Z., Wen-li, L., Han-ying, S. et al. Study on the effects of Guiqi Oral Liquid in promoting recovery of hematopoiesis in acute irradiation injured mice., Chin. J. Integr. Med. 11, 283–286 (2005). https://doi.org/10.1007/BF02835790 DOI：
Zheng Miao, Liu Wen-li, Sun Han-ying, et al. Study on the effects of Guiqi Oral Liquid in promoting recovery of hematopoiesis in acute irradiation injured mice[J]. Chinese Journal of Integrative Medicine, 2005,11(4):283-286. DOI： 10.1007/BF02835790.
摘要
Objective: To explore the effects and possible mechanisms of Guiqi Oral Liquid (GQOL) on the recovery of hematopoiesis in acute irradiation injured mice.Methods: The acute irradiation injured mice were randomly divided into 2 groups: the treated group and the control group
and also a normal control group was set up with 6 mice in it receiving no treatment. After the mice in the former two groups were irradiated by 6.0 Gy60Coγ-ray
every one of them was given 0. 4 ml GQOL or saline in equal volume through a gastric tube twice a day for 14 days. On the 4th
8th and 14th day after irradiation
the bone marrow mononuclear cells (BMMNC) and megakaryocytes in bone marrow tissues of the mice were counted
the proportion of hematopoietic tissues (by area) was measured
and the expression of adhesion molecules
CD44 and CD54
in bone marrow were estimated by immunochemistry. The colony forming unit of spleen (CFU-S) in the mice were counted on the 8th day after irradiation.Results: On the 4th
8th
14th day after irradiation
the count of BMMNC and megakaryocyte
and the proportion of hematopoietic tissues in the treated group were higher than those in the control group (P<0.01 orP<0.05). CD44 and CD54 expression in the treated group were higher than those in the control group on the 4th and 8th day (P<0.01)
but near normal on the 14th day (P<0.01). On the 8th day
CFU-S count in the treated group was higher than that in the control group (P<0.01).Conclusion: GQOL can regulate the expression of adhesion molecules
CD44 and CD54
in the bone marrow of the acute irradiation injured mice
which may be one of the mechanisms of GQOL in accelerating the early phase hematopoiesis recovery of mice.
Abstract
Objective: To explore the effects and possible mechanisms of Guiqi Oral Liquid (GQOL) on the recovery of hematopoiesis in acute irradiation injured mice.Methods: The acute irradiation injured mice were randomly divided into 2 groups: the treated group and the control group
and also a normal control group was set up with 6 mice in it receiving no treatment. After the mice in the former two groups were irradiated by 6.0 Gy60Coγ-ray
every one of them was given 0. 4 ml GQOL or saline in equal volume through a gastric tube twice a day for 14 days. On the 4th
8th and 14th day after irradiation
the bone marrow mononuclear cells (BMMNC) and megakaryocytes in bone marrow tissues of the mice were counted
the proportion of hematopoietic tissues (by area) was measured
and the expression of adhesion molecules
CD44 and CD54
in bone marrow were estimated by immunochemistry. The colony forming unit of spleen (CFU-S) in the mice were counted on the 8th day after irradiation.Results: On the 4th
8th
14th day after irradiation
the count of BMMNC and megakaryocyte
and the proportion of hematopoietic tissues in the treated group were higher than those in the control group (P<0.01 orP<0.05). CD44 and CD54 expression in the treated group were higher than those in the control group on the 4th and 8th day (P<0.01)
but near normal on the 14th day (P<0.01). On the 8th day
CFU-S count in the treated group was higher than that in the control group (P<0.01).Conclusion: GQOL can regulate the expression of adhesion molecules
CD44 and CD54
in the bone marrow of the acute irradiation injured mice
which may be one of the mechanisms of GQOL in accelerating the early phase hematopoiesis recovery of mice.
关键词
Guiqi Oral Liquidirradiation injuryCD44CD54colony forming unit of spleen
Keywords
Guiqi Oral Liquidirradiation injuryCD44CD54colony forming unit of spleen
references
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Gaugler MH, Squiban C, van der Meeren A, et al. Late and persistent up-regulation of intercellular adhesion molecule-1 (ICAM-1) expression by ionizing radiation in human endothelial cells in vitro. Int J Radiat Biol 1997; 72(2): 201–209.
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Chen T, Xie Y. Role of adhesion molecules in mobilization of hematopoietic cells. Chin Med J 2003; 116(2): 273–277.
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