Protective effects and mechanism of puerarin on learning-memory disorder after global cerebral ischemia-reperfusion injury in rats

Hai-qin Wu; He-na Guo; Hu-qing Wang; Ming-ze Chang; Gui-lian Zhang; Ying-xian Zhao

doi:10.1007/s11655-009-0054-4

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Protective effects and mechanism of puerarin on learning-memory disorder after global cerebral ischemia-reperfusion injury in rats

OriginalPaper | Updated：2021-08-27

- Protective effects and mechanism of puerarin on learning-memory disorder after global cerebral ischemia-reperfusion injury in rats
- Protective effects and mechanism of puerarin on learning-memory disorder after global cerebral ischemia-reperfusion injury in rats
- 中国结合医学杂志（英文版） 2009年15卷第1期页码：54-59
- Affiliations：
  
  Department of Neurology, the Second Affiliated Hospital, Medical College of Xi’an Jiaotong University,Xi’an,China
- Author bio：
- Funds：
  
  Supported by the Key Technologies Research and Development Program of Shaanxi Province (No. 2002k10-G2)
- DOI：10.1007/s11655-009-0054-4
  中图分类号：
- 纸质出版日期：2009，
  
  网络出版日期：2009-3-7，
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Wu, Hq., Guo, Hn., Wang, Hq. et al. Protective effects and mechanism of puerarin on learning-memory disorder after global cerebral ischemia-reperfusion injury in rats., Chin. J. Integr. Med. 15, 54–59 (2009). https://doi.org/10.1007/s11655-009-0054-4

Hai-qin Wu, He-na Guo, Hu-qing Wang, et al. Protective effects and mechanism of puerarin on learning-memory disorder after global cerebral ischemia-reperfusion injury in rats[J]. Chinese Journal of Integrative Medicine, 2009,15(1):54-59.
Wu, Hq., Guo, Hn., Wang, Hq. et al. Protective effects and mechanism of puerarin on learning-memory disorder after global cerebral ischemia-reperfusion injury in rats., Chin. J. Integr. Med. 15, 54–59 (2009). https://doi.org/10.1007/s11655-009-0054-4 DOI：

Hai-qin Wu, He-na Guo, Hu-qing Wang, et al. Protective effects and mechanism of puerarin on learning-memory disorder after global cerebral ischemia-reperfusion injury in rats[J]. Chinese Journal of Integrative Medicine, 2009,15(1):54-59. DOI： 10.1007/s11655-009-0054-4.

摘要

To observe the effect of puerarin on the learning-memory disorder after global cerebral ischemia-reperfusion injury in rats

and to explore its mechanism of action. The global cerebral ischemia-reperfusion injury model was established using the modifified Pulsinelli four-vessel occlusion in Sprague-Dawley rats. Rats were intraperitoneally injected with puerarin (100 mg/kg) 1 h before ischemia and once every 6 h afterwards. The learning-memory ability was evaluated by the passive avoidance test. The dynamic changes of the cell counts of apoptosis and positive expression of Bcl-2 in the hippocampus CA1 region were determined by the TUNEL and immunohistochemical methods

respectively. (1) Compared with the reperfusion group

the step through latency (STL) in the passive avoidance test in the puerarin group was prolonged signifificantly (P<0.01). (2) The apoptotic neurons were injured most severely on the 3rd day in the hippocampal CA1 region after global ischemia and reperfusion. In the puerarin group

the number of apoptotic cells decreased at respective time points after ischemia-reperfusion (P<0.01). (3) The level of positive expression of Bcl-2 varied according to the duration of reperfusion and the peak level occurred on day 1 in the hippocampal CA1 region after global cerebral ischemia. Compared with the reperfusion group

the expression of Bcl-2 in the puerarin group was up-regulated at the respective time points after ischemia reperfusion (P<0.01)

reaching the peak on day 1. Puerarin could improve the learning-memory ability after global cerebral ischemia and reperfusion in rats. The protective mechanism might be related to the effect of inhibiting or delaying the cell apoptosis through up-regulating the expression of Bcl-2 after ischemia and reperfusion.

Abstract

To observe the effect of puerarin on the learning-memory disorder after global cerebral ischemia-reperfusion injury in rats

respectively. (1) Compared with the reperfusion group

the expression of Bcl-2 in the puerarin group was up-regulated at the respective time points after ischemia reperfusion (P<0.01)

关键词

Puerarincerebral ischemia-reperfusion injurylearning-memory disorder

Keywords

Puerarincerebral ischemia-reperfusion injurylearning-memory disorder

references

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