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Effect of serum from overfatigue rats on JNK/c-Jun/HO-1 pathway in human umbilical vein endothelial cells and the intervening effect of Tongxinluo (通心络) superfine powder
Effect of serum from overfatigue rats on JNK/c-Jun/HO-1 pathway in human umbilical vein endothelial cells and the intervening effect of Tongxinluo (通心络) superfine powder
- 中国结合医学杂志（英文版） 2009年15卷第2期 页码：121-127
- Affiliations：
1. Department of Pharmacology, Hebei Yiling Pharmaceutical Research Institute,Shijiazhuang,China
2. Synthesized Department of the Affiliated Hospital of Chengde Medical College,Chengde,China
3. Department of Gastroenterology, Traditional Chinese Medicine Hospital of Hebei,Shijiazhuang,China
4. Department of Neurology, Traditional Chinese Medicine Hospital of Shijiazhuang,Shijiazhuang,China
- Author bio：
- Funds：Supported by the National Key Basic Research and Development Project (973 Project, No. 2005CB523301), the International Science and Technology Cooperation Program (No. 2006DFB32460)
DOI：10.1007/s11655-009-0121-0
中图分类号：纸质出版日期：2009，
网络出版日期：2009-4-29，
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Liang, Jq., Xu, Hb., Wu, Yl. et al. Effect of serum from overfatigue rats on JNK/c-Jun/HO-1 pathway in human umbilical vein endothelial cells and the intervening effect of Tongxinluo (通心络) superfine powder., Chin. J. Integr. Med. 15, 121–127 (2009). https://doi.org/10.1007/s11655-009-0121-0
Jun-qing Liang, Hai-bo Xu, Yi-ling Wu, et al. Effect of serum from overfatigue rats on JNK/c-Jun/HO-1 pathway in human umbilical vein endothelial cells and the intervening effect of Tongxinluo (通心络) superfine powder[J]. Chinese Journal of Integrative Medicine, 2009,15(2):121-127.
Liang, Jq., Xu, Hb., Wu, Yl. et al. Effect of serum from overfatigue rats on JNK/c-Jun/HO-1 pathway in human umbilical vein endothelial cells and the intervening effect of Tongxinluo (通心络) superfine powder., Chin. J. Integr. Med. 15, 121–127 (2009). https://doi.org/10.1007/s11655-009-0121-0 DOI：
Jun-qing Liang, Hai-bo Xu, Yi-ling Wu, et al. Effect of serum from overfatigue rats on JNK/c-Jun/HO-1 pathway in human umbilical vein endothelial cells and the intervening effect of Tongxinluo (通心络) superfine powder[J]. Chinese Journal of Integrative Medicine, 2009,15(2):121-127. DOI： 10.1007/s11655-009-0121-0.
摘要
To cultivate human umbilical vein endothelial cells (HUVECs) in the serum of overfatigue rats with the intervention of Tongxinluo (通心络) superfine powder (TXLSP). By examining the variation of the activity of JNK/c-Jun/HO-1 pathway
the possible mechanisms of vascular endothelial dysfunction under overfatigue conditions and the intervening effect of TXLSP were explored. The HUVECs were randomly divided into the normal control group
the model group
the SP600125 (a specifific antagonist of JNK) group
the TXLSP group and the TXLSP + SP600125 group. The content of carboyhemoglobin (COHb) and the leak rate of lactic dehydrogenase (LDH) in different groups were measured. The mRNA and protein expression of JNK
c-Jun
HO-1 and the phosphorylation level of c-Jun (P-c-Jun) were detected using Western blot and PCR methods. Compared with the normal control group
the COHb level in supernatant was increased signifificantly in the model group
and the expression of HO-1
JNK
c-Jun mRNA and corresponding proteins and P-c-Jun were also increased remarkably. The increases in these parameters were signifificantly decreased by SP600125. TXLSP showed remarkable up-regulation on the expression of JNK
c-Jun
P-c-Jun and HO-1 mRNA and their protein expression. Compared with the SP600125 group
the expressions of JNK
c-Jun
P-c-Jun and HO-1 mRNA and its protein in the TXLSP+SP600125 group were signifificantly increased at different time points (P<0.05
P<0.01). The vascular endothelial dysfunction under overfatigue conditions is related to the activity of the JNK/c-Jun/HO-1 pathway. One of the mechanisms of TXLSP in improving the vascular endothelial function is to adjust the activity of the JNK/c-Jun/HO-1 pathway at gene and protein levels.
Abstract
To cultivate human umbilical vein endothelial cells (HUVECs) in the serum of overfatigue rats with the intervention of Tongxinluo (通心络) superfine powder (TXLSP). By examining the variation of the activity of JNK/c-Jun/HO-1 pathway
the possible mechanisms of vascular endothelial dysfunction under overfatigue conditions and the intervening effect of TXLSP were explored. The HUVECs were randomly divided into the normal control group
the model group
the SP600125 (a specifific antagonist of JNK) group
the TXLSP group and the TXLSP + SP600125 group. The content of carboyhemoglobin (COHb) and the leak rate of lactic dehydrogenase (LDH) in different groups were measured. The mRNA and protein expression of JNK
c-Jun
HO-1 and the phosphorylation level of c-Jun (P-c-Jun) were detected using Western blot and PCR methods. Compared with the normal control group
the COHb level in supernatant was increased signifificantly in the model group
and the expression of HO-1
JNK
c-Jun mRNA and corresponding proteins and P-c-Jun were also increased remarkably. The increases in these parameters were signifificantly decreased by SP600125. TXLSP showed remarkable up-regulation on the expression of JNK
c-Jun
P-c-Jun and HO-1 mRNA and their protein expression. Compared with the SP600125 group
the expressions of JNK
c-Jun
P-c-Jun and HO-1 mRNA and its protein in the TXLSP+SP600125 group were signifificantly increased at different time points (P<0.05
P<0.01). The vascular endothelial dysfunction under overfatigue conditions is related to the activity of the JNK/c-Jun/HO-1 pathway. One of the mechanisms of TXLSP in improving the vascular endothelial function is to adjust the activity of the JNK/c-Jun/HO-1 pathway at gene and protein levels.
关键词
overfatigueserumvascular endothelial dysfunctionsignal conductionTongxinluo superfine powder
Keywords
overfatigueserumvascular endothelial dysfunctionsignal conductionTongxinluo superfine powder
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