Ginsenoside Rb1, a panoxadiol saponin against oxidative damage and renal interstitial fibrosis in rats with unilateral ureteral obstruction

Xi-sheng Xie; Heng-chuan Liu; Man Yang; Chuan Zuo; Yao Deng; Jun-ming Fan

doi:10.1007/s11655-009-0133-9

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Ginsenoside Rb1, a panoxadiol saponin against oxidative damage and renal interstitial fibrosis in rats with unilateral ureteral obstruction

OriginalPaper | Updated：2021-08-27

- Ginsenoside Rb1, a panoxadiol saponin against oxidative damage and renal interstitial fibrosis in rats with unilateral ureteral obstruction
- Ginsenoside Rb1, a panoxadiol saponin against oxidative damage and renal interstitial fibrosis in rats with unilateral ureteral obstruction
- 中国结合医学杂志（英文版） 2009年15卷第2期页码：133-140
- Affiliations：
  
  1. Department of Nephrology, West China Hospital, Sichuan University,Chengdu,China
  2. Department of Medicine Technology, West China School of Public Health, Sichuan University,Chengdu,China
  3. State Key Laboratory of Biotherapy of Human Disease, West China Hospital, Sichuan University,Chengdu,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-009-0133-9
  中图分类号：
- 纸质出版日期：2009，
  
  网络出版日期：2009-4-29，
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Xie, Xs., Liu, Hc., Yang, M. et al. Ginsenoside Rb1, a panoxadiol saponin against oxidative damage and renal interstitial fibrosis in rats with unilateral ureteral obstruction., Chin. J. Integr. Med. 15, 133–140 (2009). https://doi.org/10.1007/s11655-009-0133-9

Xi-sheng Xie, Heng-chuan Liu, Man Yang, et al. Ginsenoside Rb1, a panoxadiol saponin against oxidative damage and renal interstitial fibrosis in rats with unilateral ureteral obstruction[J]. Chinese Journal of Integrative Medicine, 2009,15(2):133-140.
Xie, Xs., Liu, Hc., Yang, M. et al. Ginsenoside Rb1, a panoxadiol saponin against oxidative damage and renal interstitial fibrosis in rats with unilateral ureteral obstruction., Chin. J. Integr. Med. 15, 133–140 (2009). https://doi.org/10.1007/s11655-009-0133-9 DOI：

Xi-sheng Xie, Heng-chuan Liu, Man Yang, et al. Ginsenoside Rb1, a panoxadiol saponin against oxidative damage and renal interstitial fibrosis in rats with unilateral ureteral obstruction[J]. Chinese Journal of Integrative Medicine, 2009,15(2):133-140. DOI： 10.1007/s11655-009-0133-9.

摘要

To investigate the possible protective effect and mechanism of ginsenoside Rb1 against oxidative damage and renal interstitial fibrosis on rats with unilateral ureteral obstruction (UUO). In total

80 male rats were randomly divided into 4 groups

20 in each group: the sham operated group (SOR)

UUO group

UUO with ginsenoside Rb1 treatment group (treated with intraperitoneal injection of 50 mg/ kg daily) and UUO with Losartan treatment group (as the positive control

treated with 20 mg/kg by gastrogavage per day). The rats were randomly sacrifificed on day 3

7 and 14 after surgery

respectively. The histopathologic changes of renal interstitial tissues were observed with Masson staining. The mRNA of transforming growth factor β 1 (TGF-β 1)

collagen I and fibronectin were reversed transcribed and quantified by Real-time PCR. Enzyme-linked immunosorbent assay was used to quantitatively detect TGF-β 1 and 8-hydroxy-2′-deoxyguanosine (8-OHdG) levels. P47phox protein expression was assessed by immunohistochemistry and Western blot analysis. In the UUO model

the obstructed kidney showed typical features of progressive renal tubulointerstitial fibrosis

and the levels of TGF-β1

collagen I and fibronectin increased (P<0.05). As compared with the UUO group

ginsennoside Rb1 significantly inhibited the interstitial fibrosis including tubular injury and collagen deposition

and decreased the levels of TGF-β1 (P<0.05). Ginsenoside Rb1 also inhibited the heme oxygenase (HO-1) and 8-OHdG

two markers of oxidative stress (P<0.05). Moreover

ginsenoside Rb1 suppressed the expression of p47phox

a subunit of nicotinamide adeninedinucleotide phosphate (NADPH) oxidase (P<0.05). Ginsenoside Rb1 can obviously inhibit renal interstitial fibrosis in rats with UUO

its mechanism possibly via against the oxidative damage and suppressing TGF-β1 expression.

Abstract

To investigate the possible protective effect and mechanism of ginsenoside Rb1 against oxidative damage and renal interstitial fibrosis on rats with unilateral ureteral obstruction (UUO). In total

80 male rats were randomly divided into 4 groups

20 in each group: the sham operated group (SOR)

UUO group

UUO with ginsenoside Rb1 treatment group (treated with intraperitoneal injection of 50 mg/ kg daily) and UUO with Losartan treatment group (as the positive control

treated with 20 mg/kg by gastrogavage per day). The rats were randomly sacrifificed on day 3

7 and 14 after surgery

respectively. The histopathologic changes of renal interstitial tissues were observed with Masson staining. The mRNA of transforming growth factor β 1 (TGF-β 1)

the obstructed kidney showed typical features of progressive renal tubulointerstitial fibrosis

and the levels of TGF-β1

collagen I and fibronectin increased (P<0.05). As compared with the UUO group

ginsennoside Rb1 significantly inhibited the interstitial fibrosis including tubular injury and collagen deposition

and decreased the levels of TGF-β1 (P<0.05). Ginsenoside Rb1 also inhibited the heme oxygenase (HO-1) and 8-OHdG

two markers of oxidative stress (P<0.05). Moreover

ginsenoside Rb1 suppressed the expression of p47phox

a subunit of nicotinamide adeninedinucleotide phosphate (NADPH) oxidase (P<0.05). Ginsenoside Rb1 can obviously inhibit renal interstitial fibrosis in rats with UUO

its mechanism possibly via against the oxidative damage and suppressing TGF-β1 expression.

关键词

ginsenoside Rb1unilateral ureteral obstructionoxidative stresstransforming growth factor beta1p47phox

Keywords

ginsenoside Rb1unilateral ureteral obstructionoxidative stresstransforming growth factor beta1p47phox

references

Liu Y. Renal fibrosis: new insights into the pathogenesis and therapeutics. Kidney Int 2006; 69: 213–217.

Manucha W. Biochemical-molecular markers in unilateral ureteral obstruction. Biocell 2007; 31:1–12.

Ha H, Lee HB. Reactive oxygen species amplify glucose signalling in renal cells cultured under high glucose and in diabetic kidney. Nephrology-(Carlton) 2005;10Suppl: S7–S10.

Hwang M, Kim HJ, Noh HJ, Chang YC, Chae YM, Kim KH, et al. TGF-beta1 siRNA suppresses the tubulointerstitial fi brosis in the kidney of ureteral obstruction. Exp Mol Pathol 2006; 81:48–54.

Rhyu DY, Yang Y, Ha H, Lee GT, Song JS, Uh ST, et al. Role of reactive oxygen species in TGF-β 1-induced mitogen-activated protein kinase activation and epithelial-mesenchymal transition in renal tubular epithelial cells. J Am Soc Nephrol 2005; 16:667–675.

Fan Q, Liao J, Kobayashi M, Yamashita M, Gu L, Gohda T, et al. Candesartan reduced advanced glycation endproducts accumulation and diminished nitro-oxidative stress in type 2 diabetic KK/Ta mice. Nephrol Dial Transplant 2004;19:3012–3020.

Sugiyama H, Kobayashi M, Wang DH, Sunami R, Maeshima Y, Yamasaki Y, et al. Telmisartan inhibits both oxidative stress and renal fibrosis after unilateral ureteral obstruction in acatalasemic mice. Nephrol Dial Transplant 2005; 20:2670–2680.

Li JM, Shah AM. ROS generation by nonphagocytic NADPH oxidase: potential relevance in diabetic nephropathy. J Am Soc Nephrol 2003;14: S221–S226.

Moriyama T, Kawada N, Nagatoya K, Takeji M, Horio M, Ando A, et al. Fluvastatin suppresses oxidative stress and fi brosis in the interstitium of mouse kidneys with unilateral ureteral obstruction. Kidney Int 2001;59:2095–2103.

Saborio P, Krieg RJ Jr, Kuemmerle NB, Norkus EP, Schwartz CC, Chan JC. Alpha-tocopherol modulates lipoprotein cytotoxicity in obstructive nephropathy. Pediatr Nephrol 2000;14:740–746.

Izuhara Y, Nangaku M, Inagi R, Tominaga N, Aizawa T, Kurokawa K, et al. Renoprotective properties of angiotensin receptor blockers beyond blood pressure lowering. J Am Soc Nephrol 2005; 16:3631–3641.

Asaba K, Tojo A, Onozato ML, Goto A, Quinn MT, Fujita T, et al. Effects of NADPH oxidase inhibitor in diabetic nephropathy. Kidney Int 2005; 67:1890–1898.

Xu QF, Fang XL, Chen DF. Pharmacokinetics and bioavailability of ginsenoside Rb1 and Rg1 from Panax notoginseng in rats. J Ethnopharmacol 2003; 84:187–192.

Li L, Sheng YX, Zhang JL, Wang SS, Guo DA. High-performance liquid chromatographic assay for the active saponins from Panax notoginseng in rat tissues. Biomed Chromatogr 2006; 20:327–335.

Su BH, Li Z, Fan JM, Wang M, Tang R. Effects of panax notoginseng saponins on the process of renal interstitial fibrosis after unilateral ureteral obstruction in rats. J Sichuan Univ (Natural Sci Edit, Chin) 2005; 36:368–371.

Wang M, Fan JM, Liu XY. Effect of total saponins of Panax notoginseng on transdifferentiation of rats’ tubular epithelial cell induced by IL-1 alpha. Chin J Integr Tradit West Med (Chin) 2004; 24:722–725.

Ohashi R, Yan S, Mu H, Chai H, Yao Q, Lin PH, et al. Effects of homocysteine and ginsenoside Rb1 on endothelial proliferation and superoxide anion production. J Surg Res 2006;133:89–94.

Zhou W, Chai H, Lin PH, Lumsden AB, Yao Q, Chen C. Ginsenoside Rb1 blocks homocysteine-induced endothelial dysfunction in porcine coronary arteries. J Vasc Surg 2005;41:861–868.

Ma Z, Yang Z. Scavenging effects of Astragalus and Gynostemma pentaphyllum with its product on O2− and OH. Chin Tradit Herbal Drugs (Chin) 1999; 22:303–306.

Kang KS, Lee YJ, Park JH, Yokozawa T. The effects of glycine and L-argining on heat stability of ginsenoside Rb1. Biol Pharm Bull 2007;30:1975–1978.

Morrissey JJ, Klahr S. Differential effects of ACE and AT1 receptor inhibition on chemoattractant and adhesion molecule synthesis. Am J Physiol 1998; 274:580–586.

Guo G, Morrissey J, McCracken R, Tolley T, Liapis H, Klahr S. Contributions of angiotensin II and tumor necrosis factor-alpha to the development of renal fibrosis. Am J Physiol Renal Physiol 2001; 280:777–785.

Kaneto H, Morrissey J, McCracken R, Reyes A, Klahr S. Enalapril reduces collagen type IV synthesis and expansion of the interstitium in the obstructed rat kidney. Kidney Int 1994; 45:1637–1647.

Ishidoya S, Morrissey J, McCracken R, Reyes A, Klahr S. Angiotensin II receptor antagonist ameliorates renal tubulointerstitial fibrosis caused by unilateral ureteral obstruction. Kidney Int 1995; 47:1285–1294.

Mizuguchi Y, Miyajima A, Kosaka T, Asano T, Asano T, Hayakawa M. Atorvastatin ameliorates renal tissue damage in unilateral ureteral obstruction. J Urol 2004; 172:2456–2459.

Pfaffl MW, Horgan GW, Dempfle L. Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res 2002; 30: 36.

Fu P, Liu F, Su S, Huang XR, Entman ML, Schwartz RJ, et al. Signaling mechanism of renal fibrosis in unilateral ureteral obstructive kidney disease in ROCK1 knockout mice. J Am Soc Nephrol 2006; 17:3105–3114.

Kanauchi M, Nishioka H, Hashimoto T. Oxidative DNA damage and tubulointerstitial injury in diabetic nephropathy. Nephron 2002; 91:327–329.

Boonla C, Wunsuwan R, Tungsanga K, Tosukhowong P. Urinary 8-hydroxydeoxyguanosine is elevated in patients with nephrolithiasis. Urol Res 2007; 35:185–191.

Pat B, Yang T, Kong C, Watters D, Johnson DW, Gobe G. Activation of ERK in renal fibrosis after unilateral ureteral obstruction: modulation by antioxidants. Kidney Int 2005; 67:931–943.

Moon JA, Kim HT, Cho IS, Sheen YY, Kim DK. IN-1130, a novel transforming growth factor-beta type I receptor kinase (ALK5) inhibitor, suppresses renal fibrosis in obstructive nephropathy. Kidney Int 2006;70:1234–1243.

Mizuguchi Y, Chen J, Seshan SV, Poppas DP, Szeto HH, Felsen D. A novel cell permeable antioxidant peptide decreases renal tubular apoptosis and damage in unilateral ureteral obstruction. Am J Physiol Renal Physiol 2008;295:1545–1553.

Manucha W, Oliveros L, Carrizo L, Seltzer A, Vallés P. Losartan modulation on NOS isoforms and COX-2 expression in early renal fibrogenesis in unilateral obstruction. Kidney Int 2004;65:2091–2107.

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