Imperatorin is responsible for the vasodilatation activity of Angelica Dahurica var. Formosana regulated by nitric oxide in an endothelium-dependent manner

Hong Nie; Lan-zhen Meng; Jiu-yao Zhou; Xiao-feng Fan; Yong Luo-; Guang-wen Zhang

doi:10.1007/s11655-009-0442-z

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Imperatorin is responsible for the vasodilatation activity of Angelica Dahurica var. Formosana regulated by nitric oxide in an endothelium-dependent manner

OriginalPaper | Updated：2021-08-27

- Imperatorin is responsible for the vasodilatation activity of Angelica Dahurica var. Formosana regulated by nitric oxide in an endothelium-dependent manner
- Imperatorin is responsible for the vasodilatation activity of Angelica Dahurica var. Formosana regulated by nitric oxide in an endothelium-dependent manner
- 中国结合医学杂志（英文版） 2009年15卷第6期页码：442-447
- Affiliations：
  
  1. College of Pharmacy, Jinan University,Guangzhou,China
  2. Guangzhou University of Chinese Medicine,Guangzhou,China
  3. Research Center for Analytical Sciences of Northeastern University,Shenyang,China
  4. College of Science and Engineering, Jinan University,Guangzhou,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30400596), Jinan University Natural Science Foundation (No. 51204017), and 211 Project of Jinan University
- DOI：10.1007/s11655-009-0442-z
  中图分类号：
- 纸质出版日期：2009，
  
  网络出版日期：2010-1-18，
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Nie, H., Meng, Lz., Zhou, Jy. et al. Imperatorin is responsible for the vasodilatation activity of Angelica Dahurica var. Formosana regulated by nitric oxide in an endothelium-dependent manner., Chin. J. Integr. Med. 15, 442–447 (2009). https://doi.org/10.1007/s11655-009-0442-z

Hong Nie, Lan-zhen Meng, Jiu-yao Zhou, et al. Imperatorin is responsible for the vasodilatation activity of Angelica Dahurica var. Formosana regulated by nitric oxide in an endothelium-dependent manner[J]. Chinese Journal of Integrative Medicine, 2009,15(6):442-447.
Nie, H., Meng, Lz., Zhou, Jy. et al. Imperatorin is responsible for the vasodilatation activity of Angelica Dahurica var. Formosana regulated by nitric oxide in an endothelium-dependent manner., Chin. J. Integr. Med. 15, 442–447 (2009). https://doi.org/10.1007/s11655-009-0442-z DOI：

Hong Nie, Lan-zhen Meng, Jiu-yao Zhou, et al. Imperatorin is responsible for the vasodilatation activity of Angelica Dahurica var. Formosana regulated by nitric oxide in an endothelium-dependent manner[J]. Chinese Journal of Integrative Medicine, 2009,15(6):442-447. DOI： 10.1007/s11655-009-0442-z.

摘要

To demonstrate the vasodilatation activity of the coumarin-containing Angelica dahurica var. formosana and to further analyze active components in the herb extracts. (1) The vasodilatation effects induced by different extracts (cyclohexane

ethyl acetate

acetone

methanol

95 % ethanol and water) of Angelica dahurica var. formosana on mouse thoracic aorta pre-contracted with phenylephrine were investigated. (2) The amount of imperatorin and isoimperatorin in each extract was measured by high-performance liquid chromatography. (3) The vasodilatation effects of imperatorin and isoimperatorin on mouse thoracic aorta were compared using the same in vitro method. (4) The vasodilatation mechanism of imperatorin in the mouse thoracic aorta pre-contracted with phenylephrine was studied using the methods of denuded endothelium

NG-nitro-L-arginine methylester (L-NAME

a nitric oxide synthase inhibitor)

and propranolol. (1) The cyclohexane and ethyl acetate extracts of Angelica dahurica var. formosana decreased the maximal response of phenylephrine-induced mouse thoracic aorta contraction dose-dependently

with 50% inhibiting concentration (IC50) values of 35.3±12.4 mg/L and 40.5±12.0 mg/L

respectively. The vasodilatation effect of imperatorin and isoimperatorin was dose-dependent. (2) The cyclohexane extract

showing the strongest vasodilatation effect

possessed the highest contents of imperatorin (4.09%) and isoimperatorin (0.27%

w/w). There was a correlation between the vasodilatation activity and the contents of imperatorin and isoimperatorin in the extracts. (3) The vasodilatation effect of imperatorin was about 4-fold stronger than that of isoimperatorin. (4) The vasodilatation effect of imperatorin was signifificantly attenuated to 24.88%±4.06% in the denuded endothelium group compared with the intact endothelium group. And 1 mmol/L L-NAME reduced the imperatorin-induced vasorelaxation by 32.18 %±11.29 %. The principal effective component of Angelica dahurica var. Formosana was found to be imperatorin. Imperatorin-induced vasodilatation is at least partially regulated by nitric oxide

and has no correlation to β-receptor.

Abstract

ethyl acetate

acetone

methanol

NG-nitro-L-arginine methylester (L-NAME

a nitric oxide synthase inhibitor)

with 50% inhibiting concentration (IC50) values of 35.3±12.4 mg/L and 40.5±12.0 mg/L

respectively. The vasodilatation effect of imperatorin and isoimperatorin was dose-dependent. (2) The cyclohexane extract

showing the strongest vasodilatation effect

possessed the highest contents of imperatorin (4.09%) and isoimperatorin (0.27%

and has no correlation to β-receptor.

关键词

Angelica dahurica var. formosanathoracic aortavasodilatationimperatorinisoimperatorinhigh-performance liquid chromatography

Keywords

Angelica dahurica var. formosanathoracic aortavasodilatationimperatorinisoimperatorinhigh-performance liquid chromatography

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