Anticolchicine cytotoxicity enhanced by Dan Gua-Fang (丹瓜方), a Chinese herb prescription in ECV304 in mediums
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Anticolchicine cytotoxicity enhanced by Dan Gua-Fang (丹瓜方), a Chinese herb prescription in ECV304 in mediums
Anticolchicine cytotoxicity enhanced by Dan Gua-Fang (丹瓜方), a Chinese herb prescription in ECV304 in mediums
中国结合医学杂志(英文版)2011年17卷第2期 页码:126-133
Affiliations:
1. Department of Endocrinology, People’s Hospital Affiliated to Fujian University of Traditional Chinese Medicine,Fuzhou,China
2. China Academy of Chinese Medical Sciences,Beijing,China
3. Fujian Institute of Integrative Chinese and Western Medicine,Fuzhou,China
4. Fujian University of Traditional Chinese Medicine,Fuzhou,China
Author bio:
Funds:
Supported by the China Postdoctoral Science Foundation (No. 20070410128), CHEN Ke-ji Development Fund of Integrative Medicine (No. CKJ2006001) and Science Foundation of Health Department, Fujian Province (No. WZY0618)
Heng, Xp., Chen, Kj., Hong, Zf. et al. Anticolchicine cytotoxicity enhanced by Dan Gua-Fang (丹瓜方), a Chinese herb prescription in ECV304 in mediums., Chin. J. Integr. Med. 17, 126–133 (2011). https://doi.org/10.1007/s11655-011-0646-X
Xian-pei Heng, Ke-ji Chen, Zhen-feng Hong, et al. Anticolchicine cytotoxicity enhanced by Dan Gua-Fang (丹瓜方), a Chinese herb prescription in ECV304 in mediums[J]. Chinese Journal of Integrative Medicine, 2011,17(2):126-133.
Heng, Xp., Chen, Kj., Hong, Zf. et al. Anticolchicine cytotoxicity enhanced by Dan Gua-Fang (丹瓜方), a Chinese herb prescription in ECV304 in mediums., Chin. J. Integr. Med. 17, 126–133 (2011). https://doi.org/10.1007/s11655-011-0646-XDOI:
Xian-pei Heng, Ke-ji Chen, Zhen-feng Hong, et al. Anticolchicine cytotoxicity enhanced by Dan Gua-Fang (丹瓜方), a Chinese herb prescription in ECV304 in mediums[J]. Chinese Journal of Integrative Medicine, 2011,17(2):126-133. DOI: 10.1007/s11655-011-0646-X.
Anticolchicine cytotoxicity enhanced by Dan Gua-Fang (丹瓜方), a Chinese herb prescription in ECV304 in mediums
摘要
To study the effect of anticolchicine cytotoxicity of Dan Gua-Fang (丹瓜方)
a Chinesea Chinese)
a Chinese herbal compound prescription on endothelial cells of vein (ECV304) cultivated in mediums of different glucose concentrations as well as the proliferation of those cells in the same conditions
in order to reveal the value of Dan Gua-Fang in preventing and treating endothelial damage caused by hyperglycemia in diabetes mellitus. The research was designed as three stages. The growing state and morphological changes were observed when ECV304 were cultivated in the culture mediums
which have different glucose concentrations with or without Dan Gua-Fang and at the same time with or without colchicine. (1) Dan Gua-Fang at all concentrations reduced the floating cell population of ECV304 cultivated in hyperglycemia mediums. (2) Dan Gua-Fang at all concentrations and hyperglycemia both had a function of promoting “pseudopod-like” structure formation in cultivated ECV304
but the function was not superimposed in mediums containing both hyperglycemia and Dan Gua-Fang. (3) Colchicine reduced and even vanished the “pseudopod-like” structure of the endotheliocyte apparently cultivated in mediums of hyperglycemia or with Dan Gua-Fang. The “pseudopod-like” structure of the endotheliocyte emerged quickly in Dan Gua-Fang groups after colchicine was removed
but it was not the case in hyperglycemia only without Dan Gua-Fang groups. (4) Dan Gua-Fang reduced the mortality of cells cultivated in mediums containing colchicine. The cell revived to its normal state fast after colchicine was removed. Dan Gua-Fang has the functions of promoting the formation of cytoskeleton and fighting against colchicine cytotoxicity.
Abstract
To study the effect of anticolchicine cytotoxicity of Dan Gua-Fang (丹瓜方)
a Chinesea Chinese)
a Chinese herbal compound prescription on endothelial cells of vein (ECV304) cultivated in mediums of different glucose concentrations as well as the proliferation of those cells in the same conditions
in order to reveal the value of Dan Gua-Fang in preventing and treating endothelial damage caused by hyperglycemia in diabetes mellitus. The research was designed as three stages. The growing state and morphological changes were observed when ECV304 were cultivated in the culture mediums
which have different glucose concentrations with or without Dan Gua-Fang and at the same time with or without colchicine. (1) Dan Gua-Fang at all concentrations reduced the floating cell population of ECV304 cultivated in hyperglycemia mediums. (2) Dan Gua-Fang at all concentrations and hyperglycemia both had a function of promoting “pseudopod-like” structure formation in cultivated ECV304
but the function was not superimposed in mediums containing both hyperglycemia and Dan Gua-Fang. (3) Colchicine reduced and even vanished the “pseudopod-like” structure of the endotheliocyte apparently cultivated in mediums of hyperglycemia or with Dan Gua-Fang. The “pseudopod-like” structure of the endotheliocyte emerged quickly in Dan Gua-Fang groups after colchicine was removed
but it was not the case in hyperglycemia only without Dan Gua-Fang groups. (4) Dan Gua-Fang reduced the mortality of cells cultivated in mediums containing colchicine. The cell revived to its normal state fast after colchicine was removed. Dan Gua-Fang has the functions of promoting the formation of cytoskeleton and fighting against colchicine cytotoxicity.
关键词
Dan Gua-Fangendothelial cellcolchicinediabetes mellitus
Keywords
Dan Gua-Fangendothelial cellcolchicinediabetes mellitus
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相关机构
Fujian Institute of Traditional Chinese Medicine
Xiyuan Hospital, China Academy of Chinese Medical Sciences
Department of Endocrine, People’s Hospital Affiliated to Fujian University of Traditional Chinese Medicine
Shandong University of Traditional Chinese Medicine
Department of Cardiology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine