Therapeutic and immunoregulatory effect of GATA-binding protein-3/T-box expressed in T-cells ratio of astragalus polysaccharides on 2,4,6-trinitrobenzene sulfonic acid-induced colitis in rats

Yong-jian Gao; Feng Zhu; Jia-ming Qian; Jia-yuan Dai

doi:10.1007/s11655-015-2151-0

Your Location：

Home >

Browse articles >

Therapeutic and immunoregulatory effect of GATA-binding protein-3/T-box expressed in T-cells ratio of astragalus polysaccharides on 2,4,6-trinitrobenzene sulfonic acid-induced colitis in rats

OriginalPaper | Updated：2021-08-27

- Therapeutic and immunoregulatory effect of GATA-binding protein-3/T-box expressed in T-cells ratio of astragalus polysaccharides on 2,4,6-trinitrobenzene sulfonic acid-induced colitis in rats
- Therapeutic and immunoregulatory effect of GATA-binding protein-3/T-box expressed in T-cells ratio of astragalus polysaccharides on 2,4,6-trinitrobenzene sulfonic acid-induced colitis in rats
- 中国结合医学杂志（英文版） 2016年22卷第12期页码：918-924
- Affiliations：
  
  Department of Gastroenterology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing,China
- Author bio：
- Funds：
  
  Supported by Doctoral Fund of Ministry of Education of China
- DOI：10.1007/s11655-015-2151-0
  中图分类号：
- 纸质出版日期：2016，
  
  网络出版日期：2015-8-26，
Scan for full text
Gao, Yj., Zhu, F., Qian, Jm. et al. Therapeutic and immunoregulatory effect of GATA-binding protein-3/T-box expressed in T-cells ratio of astragalus polysaccharides on 2,4,6-trinitrobenzene sulfonic acid-induced colitis in rats., Chin. J. Integr. Med. 22, 918–924 (2016). https://doi.org/10.1007/s11655-015-2151-0

Yong-jian Gao, Feng Zhu, Jia-ming Qian, et al. Therapeutic and immunoregulatory effect of GATA-binding protein-3/T-box expressed in T-cells ratio of astragalus polysaccharides on 2,4,6-trinitrobenzene sulfonic acid-induced colitis in rats[J]. Chinese Journal of Integrative Medicine, 2016,22(12):918-924.
Gao, Yj., Zhu, F., Qian, Jm. et al. Therapeutic and immunoregulatory effect of GATA-binding protein-3/T-box expressed in T-cells ratio of astragalus polysaccharides on 2,4,6-trinitrobenzene sulfonic acid-induced colitis in rats., Chin. J. Integr. Med. 22, 918–924 (2016). https://doi.org/10.1007/s11655-015-2151-0 DOI：

Yong-jian Gao, Feng Zhu, Jia-ming Qian, et al. Therapeutic and immunoregulatory effect of GATA-binding protein-3/T-box expressed in T-cells ratio of astragalus polysaccharides on 2,4,6-trinitrobenzene sulfonic acid-induced colitis in rats[J]. Chinese Journal of Integrative Medicine, 2016,22(12):918-924. DOI： 10.1007/s11655-015-2151-0.

摘要

To analyze the immunological characteristics of 2

6-trinitrobenzene sulfonic acid (TNBS)-induced colitis model and examine the therapeutic effects and mechanisms of Astragalus polysaccharides (APS) treatment. Thirty-two male specific pathogen free Spragne-Dawley rats were randomly equally assigned to four groups: control

TNBS

APS and prednisone groups. Experimental colitis was induced by enema administration of TNBS. Then rats were treated with APS (0.5 g•kg−1•day−1

once daily) or prednisone (1.0 mg•kg−1•day−1

once daily) by gavage for 14 days. Macroscopic lesion and histological damage were determined

and activity of myeloperoxidase (MPO) was measured in the colonic tissues. Expressions of T-box expressed in T-cells (T-bet) and GATA-binding protein-3 (GATA-3) were determined by immunohistochemistry analysis and western blot. Both macroscopic lesion and histological colonic damage induced by TNBS were reduced by APS and prednisone treatment. These were accompanied by significant attenuation of MPO activity (P=0.03). TNBS intervention enhanced the expression of both GATA-3 and T-bet

but the expression of T-bet was significantly enhanced than that of GATA-3

resulting in significant reduction of GATA-3/T-bet ratio (P=0.025). APS administration enhanced the expression of T-bet (P=0.04) and GATA-3 (P=0.019) in comparison to TNBS group

and resulting in an up-regulated GATA-3/T-bet ratio. Prednisone treatment inhibited both expressions; however it also resulted in up-regulation of the GATA-3/T-bet ratio. These results demonstrated that APS exerted a beneficial immune regulatory effect on experimental colitis. It promoted the expression of T helper cell 1 (Th1) and T helper cell 2 (Th2) specific transcription factors but ultimately favor a shift toward Th2 phenotype

suggesting that APS possessed therapeutic potential in experimental colitis.

Abstract

To analyze the immunological characteristics of 2

TNBS

APS and prednisone groups. Experimental colitis was induced by enema administration of TNBS. Then rats were treated with APS (0.5 g•kg−1•day−1

once daily) or prednisone (1.0 mg•kg−1•day−1

once daily) by gavage for 14 days. Macroscopic lesion and histological damage were determined

but the expression of T-bet was significantly enhanced than that of GATA-3

resulting in significant reduction of GATA-3/T-bet ratio (P=0.025). APS administration enhanced the expression of T-bet (P=0.04) and GATA-3 (P=0.019) in comparison to TNBS group

suggesting that APS possessed therapeutic potential in experimental colitis.

关键词

Astragalus polysaccharides246-trinitrobenzene sulfonic acid-induced colitisratsT-betGATA-3Chinese Medicine

Keywords

Astragalus polysaccharides246-trinitrobenzene sulfonic acid-induced colitisratsT-betGATA-3Chinese Medicine

references

MacDonald TT, Monteleone G, Pender SL. Recent developments in the immunology of inflammatory bowel disease. Scand J Immunol 2000;51:2–9.

Dharmani P, Chadee K. Biologic therapies against inflammatory bowel disease: a dysregulated immune system and the cross talk with gastrointestinal mucosa hold the key. Curr Mol Pharmacol 2008;1:195–212.

Peng SL. The T-box transcription factor T-bet in immunity and autoimmunity. Cell Mol Immunol 2006;3:87–95.

Zhu J, Yamane H, Cote-Sierra J, Guo L, Paul WE. GATA-3 promotes Th2 responses through three different mechanisms: induction of Th2 cytokine production, selective growth of Th2 cells and inhibition of Th1 cell-specifi c factors. Cell Res 2006;16:3–10.

Wang G, Liu CT, Wang ZL, Yan CL, Luo FM, Wang L, et al. Effects of Astragalus membranaceus in promoting T-helper cell type 1 polarization and interferon-gamma production by up-regulating T-bet expression in patients with asthma. Chin J Integr Med 2006;12:262–267.

Zhang W, Zhao F. Effect of supplementary therapy with astragalus on serum Th1/Th2 in children with allergic rhinitis. Chin Remed Clin (Chin) 2006;6:680–683.

Li S, Zhang Y, Zhao J. Preparation and suppressive effect of astragalus polysaccharide in glomerulonephritis rats. Int Immunopharmacol 2007;7:23–28.

Li R, Qiu S, Chen H. Immunomodulatory effects of astragalus polysaccharides on the type 1 diabetic mice. J Chin Integr Med (Chin) 2008;6:166–170.

Ko JK, Lam FY, Cheung AP. Amelioration of experimental colitis by Astragalus membranaceus through anti-oxidation and inhibition of adhesion molecule synthesis. World J Gastroenterol 2005;11:5787–5794.

Ko JK, Chik CW. The protective action of Radix astragalus membranaceus against hapten-induced colitis through modulation of cytokines. Cytokine 2009;47:85–90.

Gao YJ, Zhu F, Qian JM. Effects of astragalus polysaccharides on 2,4,6-trinitrobenzene sulfonic acid-induced colitis in rats. Chin J Clin Nutr (Chin) 2010;18:260–264.

Morris GP, Beck PL, Herridge MS, Depew WT, Szewczuk MR, Wallace JL. Hapten-induced model of chronic infl ammation and ulceration in the rat colon. Gastroenterology 1989;96:795–803.

Cooper HS, Murthy SN, Shah RS, Sedergran DJ. Clinicopathologic study of dextran sulfate sodium experimental murine colitis. Lab Invest 1993;69:238–249.

Wallace JL, Keenan CM. An orally active inhibitor of leukotriene synthesis accelerates healing in a rat model of colitis. Am J Physiol 1990;258:G527–G534.

Sykes AP, Bhogal R, Brampton C, Chander C, Whelan C, Parsons ME, et al. The effect of an inhibitor of matrix metalloproteinases on colonic inflammation in a trinitrobenzenesulphonic acid rat model of inflammatory bowel disease. Aliment Pharmacol Ther 1999;13:1535–1542.

Krawisz JE, Sharon P, Stenson WF. Quantitative assay for acute intestinal inflammation based on myeloperoxidase activity. Assessment of inflammation in rat and hamster models. Gastroenterology 1984;87:1344–1350.

Nakamura K, Honda K, Mizutani T, Akiho H, Harada N. Novel strategies for the treatment of inflammatory bowel disease: selective inhibition of cytokines and adhesion molecules. World J Gastroenterol 2006;12:4628–4635.

Waldner MJ, Neurath MF. Novel cytokine-targeted therapies and intestinal infl ammation. Curr Opin Pharmacol 2009;9:702–707.

Fuss IJ, Becker C, Yang Z, Groden C, Hornung RL, Heller F, et al. Both IL-12p70 and IL-23 are synthesized during active crohn's disease and are down-regulated by treatment with anti-IL-12 p40 monoclonal antibody. Inflamm Bowel Dis 2006;12:9–15.

Mitsuyama K, Matsumoto S, Masuda J, Yamasakii H, Kuwaki K, Takedatsu H, et al. Therapeutic strategies for targeting the IL-6/STAT3 cytokine signaling pathway in infl ammatory bowel disease. Anticancer Res 2007;27:3749–3756.

Liberman AC, Druker J, Refojo D, Holsboer F, Arzt E. Glucocorticoids inhibit GATA-3 phosphorylation and activity in T cells. FASEB J 2009;23:1558–1571.

Neurath MF, Finotto S, Glimcher LH. The role of Th1/Th2 polarization in mucosal immunity. Nat Med 2002;8:567–573.

Bowen H, Kelly A, Lee T, Lavender P. Control of cytokine gene transcription in Th1 and Th2 cells. Clin Exp Allergy 2008;38:1422–1431.

Agnello D, Lankford CS, Bream J, Morinobu A, Gadina M, O'Shea JJ, et al. Cytokines and transcription factors that regulate T helper cell differentiation: new players and new insights. J Clin Immunol 2003;23:147–161.

Chakir H, Wang H, Lefebvre DE, Webb J, Scott FW. T-bet/ GATA-3 ratio as a measure of the Th1/Th2 cytokine profi le in mixed cell populations: predominant role of GATA-3. J Immunol Methods 2003;278:157–169.

Dong L, Chen M, Zhang Q, Li LZ, Xu XQ, Xiao W. T-bet/ GATA-3 ratio is a surrogate measure of Th1/Th2 cytokine profi les and may be novel targets for CpG ODN treatment in asthma patients. Chin Med J (Engl) 2006;119:1396–1399.

Weigmann B, Neurath MF. T-bet as a possible therapeutic target in autoimmune disease. Expert Opin Ther Targets 2002;6:619–622.

Doganci A, Neurath MF, Finotto S. Mucosal immunoregulation: transcription factors as possible therapeutic targets. Curr Drug Targets Infl amm Allergy 2005;4:565–575.

Mowat AM. Anatomical basis of tolerance and immunity to intestinal antigens. Nat Rev Immunol 2003;3:331–341.

Mowat AM, Millington OR, Chirdo FG. Anatomical and cellular basis of immunity and tolerance in the intestine. J Pediatr Gastroenterol Nutr 2004;39 Suppl 3:S723–S724.

Neurath MF, Fuss I, Kelsall BL, Stuber E, Strober W. Antibodies to interleukin 12 abrogate established experimental colitis in mice. J Exp Med 1995;182:1281–1290.

FULL TEXT LINK

More>

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Future view and development of immunology: Exploring the immunology based on Chinese medicine and culture

Cost-effectiveness analysis of combined Chinese medicine and Western medicine for ischemic stroke patients

Objective tongue inspection on 142 liver cancer patients with damp-heat syndrome

Effect of Ermiao Recipe (二妙方) with medicinal guide Angelicae Pubescentis Radix on promoting the homing of bone marrow stem cells to treat cartilage damage in osteoarthritis rats

Effects of Jianpi Jiedu Recipe (健脾解毒方) on reversion of P-glycoprotein-mediated multidrug resistance through COX-2 pathway in colorectal cancer