Synergistic cytotoxicity of ampelopsin sodium and carboplatin in human non-small cell lung cancer cell line SPC-A1 by G1 cell cycle arrested

Li Lu; Li-ning Yang; Xue-xi Wang; Chun-li Song; Hong Qin; Yong-jie Wu

doi:10.1007/s11655-016-2591-1

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Synergistic cytotoxicity of ampelopsin sodium and carboplatin in human non-small cell lung cancer cell line SPC-A1 by G1 cell cycle arrested

OriginalPaper | Updated：2021-08-27

- Synergistic cytotoxicity of ampelopsin sodium and carboplatin in human non-small cell lung cancer cell line SPC-A1 by G1 cell cycle arrested
- Synergistic cytotoxicity of ampelopsin sodium and carboplatin in human non-small cell lung cancer cell line SPC-A1 by G1 cell cycle arrested
- 中国结合医学杂志（英文版） 2017年23卷第2期页码：125-131
- Affiliations：
  
  1. Department of Pharmacology, School of Medicine, Lanzhou University, Key Laboratory of Preclinical Study for New Drugs of Gansu Province,Lanzhou,China
  2. Department of Pharmacy, The Second Hospital of Gansu Province,Lanzhou,China
  3. Institute of Integrative Traditional and Western Medicine, School of Medicine, Lanzhou University,Lanzhou,China
- Author bio：
- Funds：
  
  Supported by the Natural Science Foundation of Gansu Province, China (No. 0710RJZA044)
- DOI：10.1007/s11655-016-2591-1
  中图分类号：
- 纸质出版日期：2017，
  
  网络出版日期：2016-6-14，
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Lu, L., Yang, Ln., Wang, Xx. et al. Synergistic cytotoxicity of ampelopsin sodium and carboplatin in human non-small cell lung cancer cell line SPC-A1 by G1 cell cycle arrested., Chin. J. Integr. Med. 23, 125–131 (2017). https://doi.org/10.1007/s11655-016-2591-1

Li Lu, Li-ning Yang, Xue-xi Wang, et al. Synergistic cytotoxicity of ampelopsin sodium and carboplatin in human non-small cell lung cancer cell line SPC-A1 by G1 cell cycle arrested[J]. Chinese Journal of Integrative Medicine, 2017,23(2):125-131.
Lu, L., Yang, Ln., Wang, Xx. et al. Synergistic cytotoxicity of ampelopsin sodium and carboplatin in human non-small cell lung cancer cell line SPC-A1 by G1 cell cycle arrested., Chin. J. Integr. Med. 23, 125–131 (2017). https://doi.org/10.1007/s11655-016-2591-1 DOI：

Li Lu, Li-ning Yang, Xue-xi Wang, et al. Synergistic cytotoxicity of ampelopsin sodium and carboplatin in human non-small cell lung cancer cell line SPC-A1 by G1 cell cycle arrested[J]. Chinese Journal of Integrative Medicine, 2017,23(2):125-131. DOI： 10.1007/s11655-016-2591-1.

摘要

To evaluate the cytotoxic effects of ampelopsin sodium (Amp-Na) and carboplatin (CBP) used alone or in combination on human non-small cell lung cancer (NSCLC) cells SPC-A1 in vitro and its related mechanism. Cytotoxic effects were assessed by 3-(4

5-dimethylthiazol-2-yl)-2

5-diphenyltetrazolium bromide (MTT) assays. The synergistic effects of the drugs were calculated with coefficient of drug interaction (CDI). Cell cycle was determined by flow cytometry (FCM). The levels of p53

p21

cyclinE

cyclinD1

and phosphorylated cyclin-dependent kinase-2 (p-CDK2) were evaluated by Western blot. Amp-Na (6.25–200 μg/mL) and CBP (3.13–100 μg/mL) alone exhibited prominent cytotoxic activity in a concentration-dependent manner on SPC-A1 cells with 50% inhibitive concentration values of 57.07±14.46 and 34.97±6.30 μg/mL

respectively. Drug combinations were associated with significantly higher cytotoxic effects than each drug alone (P<0.05 or 0.01). The CDI analysis confirmed the synergy of Amp-Na and CBP on inhibiting cancer cell viability across a wide concentration range (CDI <1). FCM and Western blot showed that synergistic cytotoxic effects of Amp-Na and CBP were related to G1 arrested which mainlym ediated by p 21 through the inhibition of CDK2 activity independent of the p53 tumor suppressor pathway. Amp-Na exhibits anticancer activities and enhances the antitumor activities of CBP through up-regulation of p21 and inhibition of CDK2 activity in human NSCLC cells SPC-A1. These results suggest that Amp-Na may be applied to enhance the anticancer action of CBP.

Abstract

5-dimethylthiazol-2-yl)-2

p21

cyclinE

cyclinD1

关键词

ampelopsincarboplatinhuman non-small cell lung cancercytotoxic effectp21

Keywords

ampelopsincarboplatinhuman non-small cell lung cancercytotoxic effectp21

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