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Protective Effect of Norcantharidin on Collagen-Induced Arthritis Rats
Protective Effect of Norcantharidin on Collagen-Induced Arthritis Rats
- 中国结合医学杂志（英文版） 2018年24卷第4期 页码：278-283
- Affiliations：
1. Department of Traditional Chinese Medicine, Peking University Third Hospital,Beijing,China
2. Division of Rheumatology, PLA Army General Hospital,Beijing,China
3. Institute of Basic Research in Clinical Medicine, Academy of Chinese Medical Sciences,Beijing,China
4. Division of Rheumatology, Peking University Third Hospital,Beijing,China
- Author bio：
- Funds：
DOI：10.1007/s11655-017-2792-2
中图分类号：纸质出版日期：2018，
网络出版日期：2017-12-21，
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Shen, Hb., Huo, Zj., Bai, Yj. et al. Protective Effect of Norcantharidin on Collagen-Induced Arthritis Rats., Chin. J. Integr. Med. 24, 278–283 (2018). https://doi.org/10.1007/s11655-017-2792-2
Hong-bo Shen, Ze-jun Huo, Yun-jing Bai, et al. Protective Effect of Norcantharidin on Collagen-Induced Arthritis Rats[J]. Chinese Journal of Integrative Medicine, 2018,24(4):278-283.
Shen, Hb., Huo, Zj., Bai, Yj. et al. Protective Effect of Norcantharidin on Collagen-Induced Arthritis Rats., Chin. J. Integr. Med. 24, 278–283 (2018). https://doi.org/10.1007/s11655-017-2792-2 DOI：
Hong-bo Shen, Ze-jun Huo, Yun-jing Bai, et al. Protective Effect of Norcantharidin on Collagen-Induced Arthritis Rats[J]. Chinese Journal of Integrative Medicine, 2018,24(4):278-283. DOI： 10.1007/s11655-017-2792-2.
摘要
To observe the effect of norcantharidin (NCTD) on collagen-induced arthritis (CIA) rats. Sixty Sprague-Dawley(SD) rats were randomly divided into 6 groups (n=10): normal group
CIA model group(model group)
NCTD low-dose group [1.35 mg/(kg•d)]
NCTD middle-dose group [2.7 mg/(kg•d)]
NCTD high-dose group [5.4 mg/(kg•d)] and methotrexate (MTX) group [1.8 mg/(kg/w)]. Anesthetized rats were sacrificed by luxation of cervical vertebra after 4 weeks of administration. The arthritis scores were evaluated twice a week. The pathological changes in the ankle joints of rats were observed by hematoxylin-eosin (H&E) staining. The serum levels of interleukin (IL) 1β
IL-6
tumor necrosis factor (TNF)-α
vascular endothelial growth factor (VEGF)
IL-17 and transform growth factor (TGF) β were detected by enzyme linked immunosorbent assay (ELISA). The mRNA expression of retinoid-related orphan nuclear receptorγt (RORγt) and forkhead box P3 (Foxp3) in peripheral blood lymphocytes were confirmed by real-time polymerase chain reaction. MTX and high-dose NCTD not only decreased the arthritis scores but also alleviated the pathological changes in CIA rats’ ankle joints compared with the model group (P<0.05 or P<0.01). All doses of NCTD significantly inhibited the serum levels of IL-6
IL-17 and TNF-α in CIA rats (P<0.05). Only middle- and high-dose of NCTD prominently decreased serum IL-1β and TGF-β levels of CIA rats (P<0.05). However
NCTD has no effect on vascular endothelial growth factor (VEGF) level in CIA rats. The Foxp3 mRNA expression in all NCTD groups were increased significantly than in the model group (P<0.05). The mRNA expression of RORγt in NCTD high-dose group was decreased apparently in comparison with the model group (P<0.05). NCTD showed therapeutic effect on CIA rats by inhibition of cytokines and regulation of Th17/Treg cells.
Abstract
To observe the effect of norcantharidin (NCTD) on collagen-induced arthritis (CIA) rats. Sixty Sprague-Dawley(SD) rats were randomly divided into 6 groups (n=10): normal group
CIA model group(model group)
NCTD low-dose group [1.35 mg/(kg•d)]
NCTD middle-dose group [2.7 mg/(kg•d)]
NCTD high-dose group [5.4 mg/(kg•d)] and methotrexate (MTX) group [1.8 mg/(kg/w)]. Anesthetized rats were sacrificed by luxation of cervical vertebra after 4 weeks of administration. The arthritis scores were evaluated twice a week. The pathological changes in the ankle joints of rats were observed by hematoxylin-eosin (H&E) staining. The serum levels of interleukin (IL) 1β
IL-6
tumor necrosis factor (TNF)-α
vascular endothelial growth factor (VEGF)
IL-17 and transform growth factor (TGF) β were detected by enzyme linked immunosorbent assay (ELISA). The mRNA expression of retinoid-related orphan nuclear receptorγt (RORγt) and forkhead box P3 (Foxp3) in peripheral blood lymphocytes were confirmed by real-time polymerase chain reaction. MTX and high-dose NCTD not only decreased the arthritis scores but also alleviated the pathological changes in CIA rats’ ankle joints compared with the model group (P<0.05 or P<0.01). All doses of NCTD significantly inhibited the serum levels of IL-6
IL-17 and TNF-α in CIA rats (P<0.05). Only middle- and high-dose of NCTD prominently decreased serum IL-1β and TGF-β levels of CIA rats (P<0.05). However
NCTD has no effect on vascular endothelial growth factor (VEGF) level in CIA rats. The Foxp3 mRNA expression in all NCTD groups were increased significantly than in the model group (P<0.05). The mRNA expression of RORγt in NCTD high-dose group was decreased apparently in comparison with the model group (P<0.05). NCTD showed therapeutic effect on CIA rats by inhibition of cytokines and regulation of Th17/Treg cells.
关键词
norcantharidincollagen-induced arthritiscytokineTh17/Tregforkhead box P3retinoid related orphan nuclear receptor γ t
Keywords
norcantharidincollagen-induced arthritiscytokineTh17/Tregforkhead box P3retinoid related orphan nuclear receptor γ t
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