Effect of Lang-Chuang-Ding Decoction (狼疮定汤) on DNA Methylation of CD70 Gene Promoter in Peripheral Blood Mononuclear Cells of Female Patients with Systemic Lupus Erythematosus
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Effect of Lang-Chuang-Ding Decoction (狼疮定汤) on DNA Methylation of CD70 Gene Promoter in Peripheral Blood Mononuclear Cells of Female Patients with Systemic Lupus Erythematosus
Effect of Lang-Chuang-Ding Decoction (狼疮定汤) on DNA Methylation of CD70 Gene Promoter in Peripheral Blood Mononuclear Cells of Female Patients with Systemic Lupus Erythematosus
中国结合医学杂志(英文版)2018年24卷第5期 页码:348-352
Affiliations:
1. Department of Rheumatism, the Second Hospital Affiliated to Zhejiang Chinese Medical University,Hangzhou,China
2. College of Basic Medical Science, Zhejiang Chinese Medical University,Hangzhou,China
Author bio:
Funds:
Supported by the National Natural Science Foundation of China (No. 81373633) and the Traditional Chinese Medicine Scientific Research Fund of Zhejiang Province, China (No. 2016ZA095)
Sun, J., Shao, Tj., Zhang, Dy. et al. Effect of Lang-Chuang-Ding Decoction (狼疮定汤) on DNA Methylation of CD70 Gene Promoter in Peripheral Blood Mononuclear Cells of Female Patients with Systemic Lupus Erythematosus., Chin. J. Integr. Med. 24, 348–352 (2018). https://doi.org/10.1007/s11655-017-2804-2
Jing Sun, Tie-juan Shao, Dong-yu Zhang, et al. Effect of Lang-Chuang-Ding Decoction (狼疮定汤) on DNA Methylation of CD70 Gene Promoter in Peripheral Blood Mononuclear Cells of Female Patients with Systemic Lupus Erythematosus[J]. Chinese Journal of Integrative Medicine, 2018,24(5):348-352.
Sun, J., Shao, Tj., Zhang, Dy. et al. Effect of Lang-Chuang-Ding Decoction (狼疮定汤) on DNA Methylation of CD70 Gene Promoter in Peripheral Blood Mononuclear Cells of Female Patients with Systemic Lupus Erythematosus., Chin. J. Integr. Med. 24, 348–352 (2018). https://doi.org/10.1007/s11655-017-2804-2DOI:
Jing Sun, Tie-juan Shao, Dong-yu Zhang, et al. Effect of Lang-Chuang-Ding Decoction (狼疮定汤) on DNA Methylation of CD70 Gene Promoter in Peripheral Blood Mononuclear Cells of Female Patients with Systemic Lupus Erythematosus[J]. Chinese Journal of Integrative Medicine, 2018,24(5):348-352. DOI: 10.1007/s11655-017-2804-2.
Effect of Lang-Chuang-Ding Decoction (狼疮定汤) on DNA Methylation of CD70 Gene Promoter in Peripheral Blood Mononuclear Cells of Female Patients with Systemic Lupus Erythematosus
摘要
To investigate the effect of Lang-chuang-ding Decoction (狼疮定汤
LCD) on the expression of DNA methylation of CD70 gene promoter in peripheral blood mononuclear cells (PBMCs) of females with systemic lupus erythematosus (SLE). PBMCs isolated from female patients with SLE or healthy donors were cultured and treated with LCD medicated serum or normal serum for 24 or 48 h. The mRNA expressions of CD70 gene in PBMCs were detected by reverse transcription polymerase chain reaction (PCR); the DNA methylation of the CD70 gene promoter region was detected by methylation-specific PCR. After treated with medicated serum for 48 h
the mRNA expression levels of CD70 in PBMCs of SLE patients were signifificantly higher than those of healthy donors (P<0.05); the DNA methylation levels of CD70 promoter region in PBMCs of SLE patients treated with medicated serum for 48 h were signifificantly higher than those treated with fetal bovine serum (P<0.01). LCD could inhibit CD70 gene expression in PBMCs of SLE patients by promoting the DNA methylation of CD70 gene promoter.
Abstract
To investigate the effect of Lang-chuang-ding Decoction (狼疮定汤
LCD) on the expression of DNA methylation of CD70 gene promoter in peripheral blood mononuclear cells (PBMCs) of females with systemic lupus erythematosus (SLE). PBMCs isolated from female patients with SLE or healthy donors were cultured and treated with LCD medicated serum or normal serum for 24 or 48 h. The mRNA expressions of CD70 gene in PBMCs were detected by reverse transcription polymerase chain reaction (PCR); the DNA methylation of the CD70 gene promoter region was detected by methylation-specific PCR. After treated with medicated serum for 48 h
the mRNA expression levels of CD70 in PBMCs of SLE patients were signifificantly higher than those of healthy donors (P<0.05); the DNA methylation levels of CD70 promoter region in PBMCs of SLE patients treated with medicated serum for 48 h were signifificantly higher than those treated with fetal bovine serum (P<0.01). LCD could inhibit CD70 gene expression in PBMCs of SLE patients by promoting the DNA methylation of CD70 gene promoter.
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Departement of Rheumatology, Immunology and Nephropathy, Zhejiang Provincial Hospital of Integrated Chinese and Western Medicine, Hanghzhou
Georg-August-Universität Göttingen, Göttingen
The Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics’ Ministry of Public Health
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