电针对奥沙利铂所致大鼠外周神经毒性的保护机制

Feng-jiao WANG; She SHI; Yong-qiang WANG; Ke WANG; Shen-dong FAN; Ya-nan ZHANG; Chen-chen FENG; Zi-yong JU

doi:10.1007/s11655-022-2896-1

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电针对奥沙利铂所致大鼠外周神经毒性的保护机制

Acupuncture Research | Updated：2022-09-08

- 电针对奥沙利铂所致大鼠外周神经毒性的保护机制
- Protective Mechanism of Electroacupuncture on Peripheral Neurotoxicity Induced by Oxaliplatin in Rats
- Chinese Journal of Integrative Medicine 2022年28卷第9期页码：833-839
- Affiliations：
  
  1.College of Acumox and Tuina, Shanghai University of Traditional Chinese Medicine, Shanghai (201203), China
  2.Department of Anesthesiology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai(200120), China
  3.Acupuncture Anesthesia Clinical Research Institute, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai (200437), China
- Author bio：
  
  Dr. JU Zi-yong, E-mail: juziyong@hotmail.com.
- Funds：
  
  the National Natural Science Foundation of China(81874506);the Shanghai Clinical Research Center for Acupuncture and Moxibustion(20MC1920500)
- DOI：10.1007/s11655-022-2896-1
  中图分类号：
- 纸质出版日期：2022-09，
  
  网络出版日期：2022-07-07，
  
  录用日期：2021-10-20
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Feng-jiao WANG, She SHI, Yong-qiang WANG, 等. 电针对奥沙利铂所致大鼠外周神经毒性的保护机制[J]. Chinese Journal of Integrative Medicine, 2022,28(9):833-839.

Feng-jiao WANG, She SHI, Yong-qiang WANG, et al. Protective Mechanism of Electroacupuncture on Peripheral Neurotoxicity Induced by Oxaliplatin in Rats[J]. Chinese Journal of Integrative Medicine, 2022,28(9):833-839.
Feng-jiao WANG, She SHI, Yong-qiang WANG, 等. 电针对奥沙利铂所致大鼠外周神经毒性的保护机制[J]. Chinese Journal of Integrative Medicine, 2022,28(9):833-839. DOI： 10.1007/s11655-022-2896-1.

Feng-jiao WANG, She SHI, Yong-qiang WANG, et al. Protective Mechanism of Electroacupuncture on Peripheral Neurotoxicity Induced by Oxaliplatin in Rats[J]. Chinese Journal of Integrative Medicine, 2022,28(9):833-839. DOI： 10.1007/s11655-022-2896-1.

摘要

目的:

研究电针 (EA) 对奥沙利铂诱导的大鼠周围神经病变 (OIPN) 的影响.

方法:

雄性SD大鼠按随机数字表法分为3组:对照组、OIPN组和EA (OIPN + EA)组

每组10只. 测定机械敏感度、冷敏感性和微循环血流强度的时间进程. 电镜观察背根神经节(DRG)的形态. Western blot检测DRGs中核因子E2相关因子2 (Nrf2)、血红素加氧酶-1 (HO-1)和瞬时受体电位(TRP)蛋白家族的表达水平.

结果:

EA 治疗显着降低OIPN 大鼠的机械异常性疼痛和冷异常性疼痛 (

0.01) . 值得注意的是

奥沙利铂治疗导致DRGs微循环血流受损和病理形态缺陷 (

0.01) . 而电针治疗增加了微循环血流量

减轻了奥沙利铂引起的病理变化 (

0.01) . 此外

OIPN大鼠DRGs中Nrf2和HO-1表达下调

TRP蛋白家族过度表达 (

0.01) . EA增加了DRG中Nrf2和HO-1的表达水平

降低了TRP蛋白家族的水平 (

0.05或

0.01) .

结论:

电针可能是一种潜在的 OIPN 替代疗法

其机制可能主要由恢复 Nrf2/HO-1 信号通路介导.

Abstract

Objective:

To study the effect of electroacupuncture (EA) on oxaliplatin-induced peripheral neuropathy (OIPN) in rats.

Methods:

Male Sprague-Dawley rats were equally divided into 3 groups using a random number table: the control group

the OIPN group

and the EA (OIPN + EA) group

with 10 rats in each. The time courses of mechanical

cold sensitivity

and microcirculation blood flow intensity were determined. The morphology of the dorsal root ganglion (DRG) was observed by electron microscopic examination. The protein levels of nuclear factor E2-related factor 2 (Nrf2)

heme oxygenase-1 (HO-1)

and the transient receptor potential (TRP) protein family in DRGs were assayed by Western blot.

Results:

EA treatment significantly reduced mechanical allodynia and cold allodynia in OIPN rats (

0.01). Notably

oxaliplatin treatment resulted in impaired microcirculatory blood flow and pathomorphological defects in DRGs (

0.01). EA treatment increased the microcirculation blood flow and attenuated the pathological changes induced by oxaliplatin (

0.01). In addition

the expression levels of Nrf2 and HO-1 were down-regulated

and the TRP protein family was over-expressed in the DRGs of OIPN rats (

0.01). EA increased the expression levels of Nrf2 and HO-1 and decreased the level of TRP protein family in DRG (

0.05 or

0.01).

Conclusion:

EA may be a potential alternative therapy for OIPN

and its mechanism may be mainly mediated by restoring the Nrf2/HO-1 signaling pathway.

关键词

电针奥沙利铂周围神经病变背根神经节核因子E2相关因子2 (Nrf2)

Keywords

electroacupunctureoxaliplatinperipheral neuropathydorsal root ganglionnuclear factor-erythroid-2-related factor 2

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