Xiangshao Granules Ameliorate Post-stroke Depression by Inhibiting Activation of Microglia and IDO1 Expression in Hippocampus and Prefrontal Cortex

LI Cheng-gang; XU Lu-shan; SUN Liang; XU Yu-hao; CAO Xiang; ZHAO Chen-chen; XIA Sheng-nan; ZHANG Qing-xiu; XU Yun

doi:10.1007/s11655-024-3903-5

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Xiangshao Granules Ameliorate Post-stroke Depression by Inhibiting Activation of Microglia and IDO1 Expression in Hippocampus and Prefrontal Cortex

Original Article | Updated：2024-12-25

- Xiangshao Granules Ameliorate Post-stroke Depression by Inhibiting Activation of Microglia and IDO1 Expression in Hippocampus and Prefrontal Cortex
- Xiangshao Granules Ameliorate Post-stroke Depression by Inhibiting Activation of Microglia and IDO1 Expression in Hippocampus and Prefrontal Cortex
- 中国结合医学杂志（英文版） 2025年31卷第1期页码：28-38
- Affiliations：
  
  1.Department of Neurology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing (210008), China
  2.State Key Laboratory of Pharmaceutical Biotechnology and Institute of Translational Medicine for Brain Critical Diseases, Nanjing University, Nanjing (210008), China
  3.Jiangsu Key Laboratory for Molecular Medicine, Medical School of Nanjing University, Nanjing (210008), China
  4.Nanjing Neurology Medical Center, Nanjing (210008), China
- Author bio：
  
  Dr. XU Yun, E-mail: xuyun20042001@aliyun.com
- Funds：
  
  the National Natural Science Foundation of China(82130036;81920108017);Jiangsu Province Key Medical Discipline(ZDXKA2016020);the Key Research and Development Program of Jiangsu Province of China(BE2020620)
- DOI：10.1007/s11655-024-3903-5
  中图分类号：
- 纸质出版日期：2025-01，
  
  网络出版日期：2024-10-02，
  
  录用日期：2023-06-07
- Accepted：
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Xiangshao Granules Ameliorate Post-stroke Depression by Inhibiting Activation of Microglia and IDO1 Expression in Hippocampus and Prefrontal Cortex[J]. 中国结合医学杂志（英文版）, 2025,31(1):28-38.

LI CHENG-GANG, XU LU-SHAN, SUN LIANG, et al. Xiangshao Granules Ameliorate Post-stroke Depression by Inhibiting Activation of Microglia and IDO1 Expression in Hippocampus and Prefrontal Cortex. [J]. Chinese journal of integrative medicine, 2025, 31(1): 28-38.
Xiangshao Granules Ameliorate Post-stroke Depression by Inhibiting Activation of Microglia and IDO1 Expression in Hippocampus and Prefrontal Cortex[J]. 中国结合医学杂志（英文版）, 2025,31(1):28-38. DOI： 10.1007/s11655-024-3903-5.

LI CHENG-GANG, XU LU-SHAN, SUN LIANG, et al. Xiangshao Granules Ameliorate Post-stroke Depression by Inhibiting Activation of Microglia and IDO1 Expression in Hippocampus and Prefrontal Cortex. [J]. Chinese journal of integrative medicine, 2025, 31(1): 28-38. DOI： 10.1007/s11655-024-3903-5.

摘要

Abstract

Objective:

To investigate the therapeutic effect of Xiangshao Granules (XSG) on post-stroke depression (PSD) and explore the underlying mechanisms.

Methods:

Forty-three C57BL/6J mice were divided into 3 groups: sham (

=15)

PSD+vehicle (

=14)

and PSD+XSG (

=14) groups according to a random number table. The PSD models were constructed using chronic unpredictable mild stress (CUMS) after middle cerebral artery occlusion (MCAO). The sham group only experienced the same surgical operation

but without MACO and CUMS stimulation. The XSG group received XSG (60 mg/kg per day) by gavage for 4 weeks. The mice in the sham and vehicle groups were given the same volume of 0.9% saline at the same time. The body weight and behavior tests including open field test

sucrose preference test

tail suspension test

and elevated plus-maze test

were used to validate the PSD mouse model. Real-time fluorescence quantitative polymerase chain reaction (RT-qPCR)

enzyme-linked immunosorbent assay (ELISA)

and immunofluorescence staining were used to evaluate the anti-inflammatory effects of XSG. The potential molecular mechanisms were explored and verified through network pharmacology analysis

Nissl staining

Western blot

ELISA

and RT-qPCR

respectively.

Results:

The body weight and behavior tests showed that MCAO combined with CUMS successfully established the PSD models. XSG alleviated neuronal damage

reduced the expressions of pro-apoptotic proteins Caspase-3 and B-cell lymphoma-2 (BCL-2)-associated X (BAX)

and increased the expression of anti-apoptotic protein BCL-2 in PSD mice (

0.05

0.01). XSG inhibited microglial activation and the expressions of pro-inflammatory cytokines including tumor necrosis factor-α

interleukin (IL)-1β

and IL-6 via the toll-like receptor 4/nuclear factor kappa-B signaling pathway in PSD mice (

0.05 or

0.01). Furthermore

XSG decreased the expression of indoleamine 2

3-dioxygenase1 (IDO1) and increased the concentration of 5-hydroxytryptamine in PSD mice (

0.05 or

0.01).

Conclusion:

XSG could reverse the anxiety/depression-like behaviors and reduce the neuronal injury in the hippocampus and prefrontal cortex of PSD mice

which may be a potential therapeutic agent for PSD.

关键词

Keywords

post-stroke depressionXiangshao Granulesindoleamine 2;3-dioxygenase 1Chinese medicine

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