Study on the therapeutic mechanism of the active principle of the Chinese drug Paeoniae Radix 801 through affinity biosensors IAsys Plus quartz crystal microbalance

Huang Jia-dong; Song Zhao; Li Jing; Wu Bao-yan; Yin Hui-jun; Chen Ke-ji; Chen Qiang

doi:10.1007/BF02835747

Your Location：

Home >

Browse articles >

Study on the therapeutic mechanism of the active principle of the Chinese drug Paeoniae Radix 801 through affinity biosensors IAsys Plus quartz crystal microbalance

Updated：2021-08-27

- Study on the therapeutic mechanism of the active principle of the Chinese drug Paeoniae Radix 801 through affinity biosensors IAsys Plus quartz crystal microbalance
- Chinese Journal of Integrative Medicine Vol. 11, Issue 1, Pages: 37-40(2005)
- Affiliations：
  
  1. College of Life Sciences, Nankai University,Tianjin
  2. Xiyuan Hospital, China Academy of Traditional Chinese Medicine,China
- Author bio：
- Funds：
  
  Supported by the National Nature Science Foundation of China,(No. 90209054)
- DOI：10.1007/BF02835747
  CLC：
- Published：2005，
Scan for full text
Jia-dong, H., Zhao, S., Jing, L. et al. Study on the therapeutic mechanism of the active principle of the Chinese drug Paeoniae Radix 801 through affinity biosensors IAsys Plus quartz crystal microbalance., Chin. J. Integr. Med. 11, 37–40 (2005). https://doi.org/10.1007/BF02835747

Huang Jia-dong, Song Zhao, Li Jing, et al. Study on the therapeutic mechanism of the active principle of the Chinese drug Paeoniae Radix 801 through affinity biosensors IAsys Plus quartz crystal microbalance. [J]. Chinese Journal of Integrative Medicine 11(1):37-40(2005)
Jia-dong, H., Zhao, S., Jing, L. et al. Study on the therapeutic mechanism of the active principle of the Chinese drug Paeoniae Radix 801 through affinity biosensors IAsys Plus quartz crystal microbalance., Chin. J. Integr. Med. 11, 37–40 (2005). https://doi.org/10.1007/BF02835747 DOI：

Huang Jia-dong, Song Zhao, Li Jing, et al. Study on the therapeutic mechanism of the active principle of the Chinese drug Paeoniae Radix 801 through affinity biosensors IAsys Plus quartz crystal microbalance. [J]. Chinese Journal of Integrative Medicine 11(1):37-40(2005) DOI： 10.1007/BF02835747.

摘要

Objective: To study the targeted point and mechanism of the function of the blood-activating and stasis-removing Chinese drugs

Paeoniae Radix 801(PR801) in its cardiovascular protective effects and its specific binding with endothelin 1(ET-1) as well as the dynamics of the two’s interactive function by means of using affinity biosensors: IAsys Plus and quartz crystal microbalance (IAQCM).Methods: ET-1 was immobilized on the surfaces of IAQCM by using the new surface modification methods. The PR801 in the solution was detected by modified substrates and the specific binding between PR801 and ET-1 was studied.Results: The curves went up or down after adding PR801. There is specific binding between PR801 and ET-1. The bound mass were 0.458 ng/mm2 and 133. 54 ng/cm2

respectively. There exists relatively good stability with these two methods.Conclusion: The affinity biosensors: IAQCM can be used to study the interaction mechanism between PR801 and ET-1

providing a new way to study the interaction mechanism of TCM. PR801 can bind ET-1 specifically in the experiments. Therefore

ET-1 is another target that PR801 can bind specifically besides thromboxane A2.

Abstract

Objective: To study the targeted point and mechanism of the function of the blood-activating and stasis-removing Chinese drugs

respectively. There exists relatively good stability with these two methods.Conclusion: The affinity biosensors: IAQCM can be used to study the interaction mechanism between PR801 and ET-1

providing a new way to study the interaction mechanism of TCM. PR801 can bind ET-1 specifically in the experiments. Therefore

ET-1 is another target that PR801 can bind specifically besides thromboxane A2.

关键词

activating blood circulation to remove stasisPaeoniae Radix 801endothelin-1affinity biosensors

Keywords

activating blood circulation to remove stasisPaeoniae Radix 801endothelin-1affinity biosensors

references

Liu XL, Tang R, Zhang FH. Clinical observations and nursing of PR801 parenteral solution on treatment for ischemic cardio-cerebral vascular diseases. Heilongjiang Med J 2000;24(7): 55.

Liu Z, Zhang GS. Curative effect observations on thrombotic deep phlebitis by PR801. Chin Country Med J 1994;22(4): 50.

Chen F. Observation of compatibility of PR801 with 4 transfusions. J Chin Physician 2002;5(2): 89.

Wang YG, Chen YL, Wang SF, et al. Curative effect observations of Paeoniae Radix 801 on thrombus deep phlebitis PR801. Renshen Yanjiu 2001;13(3): 45.

Zuo XL, Zhuang HY. Clinical observation of propyl gallate in treating coronary arteriosclerotic cardiopathy angina pectoris. Acad J Qiqihar Med Coll 2002;23(1): 37.

He HJ, Fu J, Fu J, et al. Experimental study of effects of removing stasis on the level of endothelin in blood stasis syndrome. J Tanjin Coll TCM 2000;19(1): 42–45.

Yuan L, Miao ZZ, Zhao P, et al. Effects of Puerarin on blood ET-1, SOD, MDA in the patients with stroke or hypertension. Modern J Integr Tradit West Med 2000;9(22): 2197–2199.

Zou Q, Wang CG, Qiu YZ, et al. Clinical observations on treatment for acute paroxysm phase of chronic cor pulmonale with integrated traditional Chinese and western medicine. Practical Clin Emergency J Integr Trad Chin West Med 1999;6(3): 126–128.

Wu XF, Xu YX, Shen GX, et al. Surface plasmon resonance analysis to evaluate the importance of heparin sulfate groups’ binding with human aFGF and bFGF. J Zhejiang Univ Sci 2003;4(1): 86–94.

GZ. Sauerbrey. Use of crystal vibrator for weighting thin films on a microbalance. Z Phys 1959;155: 206–222.

C KöB linger, E Uttenthaler, S Drost, et al. Comparison of the QCM and the SPR method for surface studies and immunological applications. Sens Actuators B 1995; (25): 107.

BD Spangler, EA Wilkinson, JT Murphy, et al, Comparison of the Spreeta (R) surface plasmon resonance sensor and a quartz crystal microbalance for detection ofEscherichia coli heat-labile enterotoxin. Anal Chim Acta 2001, 444(1): 149–161.

X Su, S O’Shea, Determination of monoenzyme- and bienzyme stimulated precipitation by a cuvette-based surface plasmon resonance instrument. Anal Biochem 2001; 299(2): 241.

TP Vikinge, KM Hansson, P Sandströ, et al. Comparison of surface plasmon resonance and quartz crystal microbalance in the study of whole blood and plasma coagulation. Biosens Bioelectron 2000; 15 (11–12): 605–613.

FULL TEXT LINK

More>

Views

379

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Activating blood circulation to remove stasis treatment of hypertensive intracerebral hemorrhage: A multi-center prospective randomized open-label blinded-endpoint trial

Effect of electroacupuncture stimulation on expression of angiotensinogen, angiotensin II type 1 receptor, endothelin-1, and endothelin a receptor mRNA in spontaneously hypertensive rat aorta

Related Author

No data

Related Institution

Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine

Tongzhou Traditional Chinese Medicine Hospital of Beijing

Department of Traditional Chinese Medicine, the Third Hospital of Peking University

Department of Integration of Chinese and Western Medicine, Peking University Health Science Center

Address：1 Caochang, xiyuan, Beijing, People's Republic of China Postal code：100091
Tel：010-62886827, 62877592 Fax：010-62874291
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05067934号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰