Effects of Total Flavonoids ofHippophae Rhamnoides L. on intracellular free calcium in cultured vascular smooth muscle cells of spontaneously hypertensive rats and Wistar-Kyoto rats

Zhu Fu; Huang Bo; Hu Chun-yan; Jiang Qing-yuan; Lu Zhen-guo; Lu Ming; Wang Mei-hua; Gong Min; Qiao Chun-ping; Chen Wei; Huang Pan-hua

doi:10.1007/BF02835791

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Effects of Total Flavonoids ofHippophae Rhamnoides L. on intracellular free calcium in cultured vascular smooth muscle cells of spontaneously hypertensive rats and Wistar-Kyoto rats

Updated：2021-08-27

- Effects of Total Flavonoids ofHippophae Rhamnoides L. on intracellular free calcium in cultured vascular smooth muscle cells of spontaneously hypertensive rats and Wistar-Kyoto rats
- Chinese Journal of Integrative Medicine Vol. 11, Issue 4, Pages: 287-292(2005)
- Affiliations：
  
  1. Cardiovascular Department, Nanhui District Central Hospital,Shanghai
  2. Cardiovascular Department, Shanghai Renhe Hospital,China
  3. Internal Medicine Department, Longhua Hospital, Shanghai University of Traditional Chinese Medicine,China
- Author bio：
- Funds：
  
  Supported by One-hundred-people Plan of Hygiene System in Shanghai (No. 990122)
- DOI：10.1007/BF02835791
  CLC：
- Published：2005，
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Fu, Z., Bo, H., Chun-yan, H. et al. Effects of Total Flavonoids ofHippophae Rhamnoides L. on intracellular free calcium in cultured vascular smooth muscle cells of spontaneously hypertensive rats and Wistar-Kyoto rats., Chin. J. Integr. Med. 11, 287–292 (2005). https://doi.org/10.1007/BF02835791

Zhu Fu, Huang Bo, Hu Chun-yan, et al. Effects of Total Flavonoids ofHippophae Rhamnoides L. on intracellular free calcium in cultured vascular smooth muscle cells of spontaneously hypertensive rats and Wistar-Kyoto rats. [J]. Chinese Journal of Integrative Medicine 11(4):287-292(2005)
Fu, Z., Bo, H., Chun-yan, H. et al. Effects of Total Flavonoids ofHippophae Rhamnoides L. on intracellular free calcium in cultured vascular smooth muscle cells of spontaneously hypertensive rats and Wistar-Kyoto rats., Chin. J. Integr. Med. 11, 287–292 (2005). https://doi.org/10.1007/BF02835791 DOI：

Zhu Fu, Huang Bo, Hu Chun-yan, et al. Effects of Total Flavonoids ofHippophae Rhamnoides L. on intracellular free calcium in cultured vascular smooth muscle cells of spontaneously hypertensive rats and Wistar-Kyoto rats. [J]. Chinese Journal of Integrative Medicine 11(4):287-292(2005) DOI： 10.1007/BF02835791.

摘要

Objective: To explore the effects of total flavonoids ofHippophae rhamnoides L. (TFH)

quercetin (Que) and isorhamnetin (Isor) on the intracellular free calcium ([Ca2+ ]i) in vascular smooth muscle cells (VSMC) of spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats (WKY).Methods: Fluo 3-acetoxymethylester(Fluo-3/AM) was used to observe the effects of TFH (100mg/L) and its essential monomers

namely Que (l0-4 mol/L) and Isor (l0-4 mol/L) on changes of [Ca2+]i in cultured SHR and WKY VSMC (abbr. to Ca-SHR & Ca-WKY) following exposure to high K+

norepinephrine (NE) and angiotensin II (Ang II)

and to compare with the effects of verapamil (Ver).Results: (1) TFH

Que and Isor had inhibitory effects on resting Ca-SHR (P<0.05)

but had no significant effects on Ca-WKY (P>0.05). (2) High K+ could increase Ca-SHR more significantly than Ca-WKY (P<0.05); TFH

Que and Isor could inhibit the elevation of [Ca2+]i induced by high K+-depolarization

with the effects similar to that of Ver

and the effect on Ca-SHR was more significant than that on Ca-WKY (P<0.05). (3) NE and Ang II could increase Ca-SHR more significantly than Ca-WKY (P<0.05)

TFH

Que and Isor had remarkably inhibitory effect on the elevation of Ca-SHR and Ca-WKY induced by NE or Ang II. (4) In the absence of extracellular Ca2+

TFH

Que and Isor also had certain inhibitory effect on Ca-SHR and Ca-WKY induced by NE

and the effect on the former was more significant than that on the latter(P<0. 05).Conclusion: TFH

Que and lsor might decrease the levels of [Ca2+]i in VSMCs by blocking both voltage-dependent calcium channels (VDC) and receptor-operated calcium channels (ROC) in physiological or pathological state

which may be one of the important mechanisms of their hypotensive and protective effects on target organs in patients with hypertension.

Abstract

Objective: To explore the effects of total flavonoids ofHippophae rhamnoides L. (TFH)

namely Que (l0-4 mol/L) and Isor (l0-4 mol/L) on changes of [Ca2+]i in cultured SHR and WKY VSMC (abbr. to Ca-SHR & Ca-WKY) following exposure to high K+

norepinephrine (NE) and angiotensin II (Ang II)

and to compare with the effects of verapamil (Ver).Results: (1) TFH

Que and Isor had inhibitory effects on resting Ca-SHR (P<0.05)

but had no significant effects on Ca-WKY (P>0.05). (2) High K+ could increase Ca-SHR more significantly than Ca-WKY (P<0.05); TFH

Que and Isor could inhibit the elevation of [Ca2+]i induced by high K+-depolarization

with the effects similar to that of Ver

and the effect on Ca-SHR was more significant than that on Ca-WKY (P<0.05). (3) NE and Ang II could increase Ca-SHR more significantly than Ca-WKY (P<0.05)

TFH

Que and Isor had remarkably inhibitory effect on the elevation of Ca-SHR and Ca-WKY induced by NE or Ang II. (4) In the absence of extracellular Ca2+

TFH

Que and Isor also had certain inhibitory effect on Ca-SHR and Ca-WKY induced by NE

and the effect on the former was more significant than that on the latter(P<0. 05).Conclusion: TFH

which may be one of the important mechanisms of their hypotensive and protective effects on target organs in patients with hypertension.

关键词

total flavonoids ofHippophae rhamnoides Lspontaneously hypertensive ratsWistar-Kyoto ratsvascular smooth muscle cellsIntracellular Free Calcium

Keywords

total flavonoids ofHippophae rhamnoides Lspontaneously hypertensive ratsWistar-Kyoto ratsvascular smooth muscle cellsIntracellular Free Calcium

references

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Xiyuan Hospital, China Academy of Chinese Medical Sciences

Research Department, China Academy of Chinese Medical Sciences

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Faculty of TCM, The First Military Medical University

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