Effect of electroacupuncture on expression of p53 protein in cerebral cortex of rats with global cerebral ischemia/reperfusion injury

Bu Yuan; Gen De-qing; Ge Wei; Xu Xing-shun; Zeng Yin-ming

doi:10.1007/BF02836387

Your Location：

Home >

Browse articles >

Effect of electroacupuncture on expression of p53 protein in cerebral cortex of rats with global cerebral ischemia/reperfusion injury

Updated：2021-08-27

- Effect of electroacupuncture on expression of p53 protein in cerebral cortex of rats with global cerebral ischemia/reperfusion injury
- Chinese Journal of Integrative Medicine Vol. 10, Issue 2, Pages: 135-137(2004)
- Affiliations：
  
  1. Xuzhou Medical College,China
  2. Internal Neurological Department, Affiliated Hospital of Xuzhou Medical College,China
  3. Key Laboratory of anesthesiology, Xuzhou Medical College,China
- Author bio：
- Funds：
  
  This item was supported by Fund on Opening Subject of Key Laboratory in Jiangsu Province (No.K9842)
- DOI：10.1007/BF02836387
  CLC：
- Published：2004，
Scan for full text
Yuan, B., De-qing, G., Wei, G. et al. Effect of electroacupuncture on expression of p53 protein in cerebral cortex of rats with global cerebral ischemia/reperfusion injury., Chin. J. Integr. Med. 10, 135–137 (2004). https://doi.org/10.1007/BF02836387

Bu Yuan, Gen De-qing, Ge Wei, et al. Effect of electroacupuncture on expression of p53 protein in cerebral cortex of rats with global cerebral ischemia/reperfusion injury. [J]. Chinese Journal of Integrative Medicine 10(2):135-137(2004)
Yuan, B., De-qing, G., Wei, G. et al. Effect of electroacupuncture on expression of p53 protein in cerebral cortex of rats with global cerebral ischemia/reperfusion injury., Chin. J. Integr. Med. 10, 135–137 (2004). https://doi.org/10.1007/BF02836387 DOI：

Bu Yuan, Gen De-qing, Ge Wei, et al. Effect of electroacupuncture on expression of p53 protein in cerebral cortex of rats with global cerebral ischemia/reperfusion injury. [J]. Chinese Journal of Integrative Medicine 10(2):135-137(2004) DOI： 10.1007/BF02836387.

摘要

Objective: To observe the effect of electroacupuncture (EA) on expression of p53 protein in cerebral cortex of senile rats with global cerebral ischemia/reperfusion (IR) injury and to explore its mechanism.Methods: The cerebral IR injury rat model was established referring to Pulsinelli 4-vessel occlusion method. Thirty-six SD rats were randomly and evenly divided into the control group

the IR group and the IR plus EA (IR-EA) group. The animals in the control group were subjected to electrocauterization of vertebral arteries in bilateral flank orifice alone with the general carotid arteries unoccluded. To rats in the IR-EA group

immediately and 24h

48h

72h after cerebral IR

EA treatment on bilateral acupoint “Zusanli” (ST36) was applied once a day

lasting for 60 minutes. After the final treatment

all the rats were sacrificed and their brains were taken to examine p53 protein expression by the immunohistochemical method.Results: Cells with positive p53 immunoreactivity in the cerebral cortex of rats in the IR group was significantly higher than that in the control group (P<0.05)

while that in the IR-EA group was significantly lower than that in the IR group (P<0.05).Conclusion: EA could remarkably reduce expression of p53 protein in the cerebral cortex of senile rats with global cerebral IR injury

which might be one of the means for EA to inhibit neuronal apoptosis after cerebral IR injury.

Abstract

immediately and 24h

48h

72h after cerebral IR

EA treatment on bilateral acupoint “Zusanli” (ST36) was applied once a day

lasting for 60 minutes. After the final treatment

which might be one of the means for EA to inhibit neuronal apoptosis after cerebral IR injury.

关键词

electroacupuncturecerebral ischemia/reperfusionp53 protein

Keywords

electroacupuncturecerebral ischemia/reperfusionp53 protein

references

Yan YP, Sun FY. The effect of electroacupuncture on neuronal apoptosis after cerebral ischemia/reperfusion. Acupuncture Research 1998; 23(2): 33–35.

Watanabe H, Ohta S, Kumon Y, et al. Increase in p53 protein expression following cortical infarction in the spontaneously hypertensive rat. Brain Res 1997; 837(1 -2): 38–45.

Pulsinelli WA, Brierley JB. A new model of bilateral hemispheric ischemia in the unanesthetized rat. Stroke 1979;10(3): 267–272.

Qiu ML. Subject of experimental acupuncture. Shanghai: Shanghai Publishing House of Science and Technology, 1995: 2–3.

Ashcroft M, Vousden KH. Regulation of p53 stability. Oncogene 1999;18(53): 7637–7643.

van Lookeren Campagne M, Gill R. Cell cycle-related gene expression in the adult rat brain: selective induction of cyclin Gl and p21 WAF1/CIP1 in neurons following focal cerebral ischemia. Neuroscience 1998; 84(4): 1097–1112.

Selvakumaran M, Lin HK, Miyashita T, et al. Immediate early up-regulation of bax expression by p53 but not TGF beta 1: a paradigm for distinct apoptotic pathways. Oncogene 1994; 9(6): 1791–1798.

Miyashita T, Harigai M, Hanada M, et al. Identification of a p53-dependent negative response element in the bcl-2 gene. Cancer Res 1994; 54(12): 3131–3135.

Wei XE, Geng DQ, Liu YH. Experimental study on relationship between neuronal apoptosis in frontal cortex of rats after global cerebral ischemia/reperfusion injury and expression of p53 protein. Henan Practical Journal on Neuropathy 2003;6(3): 1–3.

FULL TEXT LINK

More>

Views

387

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Improved mesenchymal stem cell survival in ischemic heart through electroacupuncture

Effect of electroacupuncture on the pathomorphology of the sciatic nerve and the sensitization of P2X3 receptors in the dorsal root ganglion in rats with chronic constrictive injury

Electroacupuncture on PC6 prevents opioid-induced nausea and vomiting after laparoscopic surgery

Effects of electroacupuncture combined psycho-intervention on cognitive function and event-related potentials P300 and mismatch negativity in patients with internet addiction

Related Author

No data

Related Institution

Department of Integrated Chinese and Western Medicine, West China Hospital of Sichuan University

Institute of Cardiovascular Diseases, Pingjin Hospital, Medical College of Armed Police Forces

School of Nursing, Guangzhou University of Chinese Medicine

School of Fundamental Medical Sciences, Guangzhou University of Chinese Medicine

State Key Laboratory of Medicinal Chemical Biology, College of Life Science, Nankai University

Address：1 Caochang, xiyuan, Beijing, People's Republic of China Postal code：100091
Tel：010-62886827, 62877592 Fax：010-62874291
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05067934号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰