Study on Taxol in inhibiting human leukemia cell proliferation and inducing apoptosis

Zhao Xiao-ying; Zhang Xiao-hong; Xu Lei; Zhang Xing

doi:10.1007/BF02836412

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Study on Taxol in inhibiting human leukemia cell proliferation and inducing apoptosis

Updated：2021-08-27

- Study on Taxol in inhibiting human leukemia cell proliferation and inducing apoptosis
- Chinese Journal of Integrative Medicine Vol. 10, Issue 3, Pages: 218-220(2004)
- Affiliations：
  
  Department of Hematology, The Second Hospital, College of Medicine, Zhejiang University,Hangzhou,China
- Author bio：
- Funds：
- DOI：10.1007/BF02836412
  CLC：
- Published：2004，
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Xiao-ying, Z., Xiao-hong, Z., Lei, X. et al. Study on Taxol in inhibiting human leukemia cell proliferation and inducing apoptosis., Chin. J. Integr. Med. 10, 218–220 (2004). https://doi.org/10.1007/BF02836412

Zhao Xiao-ying, Zhang Xiao-hong, Xu Lei, et al. Study on Taxol in inhibiting human leukemia cell proliferation and inducing apoptosis. [J]. Chinese Journal of Integrative Medicine 10(3):218-220(2004)
Xiao-ying, Z., Xiao-hong, Z., Lei, X. et al. Study on Taxol in inhibiting human leukemia cell proliferation and inducing apoptosis., Chin. J. Integr. Med. 10, 218–220 (2004). https://doi.org/10.1007/BF02836412 DOI：

Zhao Xiao-ying, Zhang Xiao-hong, Xu Lei, et al. Study on Taxol in inhibiting human leukemia cell proliferation and inducing apoptosis. [J]. Chinese Journal of Integrative Medicine 10(3):218-220(2004) DOI： 10.1007/BF02836412.

摘要

Objective: To explore the effects of Taxol in inhibiting human leukemia k562 cell proliferation and inducing apoptosis in vitro.Methods: Human leukemia K562 cells were treated with Taxol of different concentrations for 12–72 hrs. Cell proliferation was evaluated by MTT assay and morphological changes of apoptosis were examined by microscopy. Cell apoptosis was determined by flow cytometry (FCM) and DNA gel electrophoresis.Results: Growth of K562 cells was inhibited by Taxol with an IC50 value of 0. 84 ⧎g/ml. Typical nuclear condensation and apoptosis bodies were observed as early as 24 hrs after a 0.5 ⧎g/ml Taxol treatment; Apoptotic rate of the Taxol-treated K562 cells increased from 3.7% to 24.0% in 24 hrs. No DNA ladder was observed by DNA gel electrophoresis.Conclusion: Taxol could inhibit K562 cell growth and induce apoptosis in vitro.

Abstract

关键词

TaxolLeukemiaK562 cell linecell apoptosis

Keywords

TaxolLeukemiaK562 cell linecell apoptosis

references

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Department of Clinical Molecular Genetics, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences

National Therapeutic Center of Hematology of Traditional Chinese Medicine, Xiyuan Hospital, China Academy of Chinese Medical Sciences

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