Effect of Bushen Yiqi Hexue recipe on pinopodes expression on endometrial surface in embryo implantation dysfunctional mice

Huang Dong-mei; Huang Guang-ying; Lu Fu-er

doi:10.1007/BF02836430

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Effect of Bushen Yiqi Hexue recipe on pinopodes expression on endometrial surface in embryo implantation dysfunctional mice

Updated：2021-08-27

- Effect of Bushen Yiqi Hexue recipe on pinopodes expression on endometrial surface in embryo implantation dysfunctional mice
- Chinese Journal of Integrative Medicine Vol. 10, Issue 4, Pages: 279-283(2004)
- Affiliations：
  
  Institute of Integrated Traditional and Western Medicine, Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan
- Author bio：
- Funds：
  
  Project supported by the National Natural Science Foundation of China (No. 30171193)
- DOI：10.1007/BF02836430
  CLC：
- Published：2004，
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Dong-mei, H., Guang-ying, H. & Fu-er, L. Effect of Bushen Yiqi Hexue recipe on pinopodes expression on endometrial surface in embryo implantation dysfunctional mice., Chin. J. Integr. Med. 10, 279–283 (2004). https://doi.org/10.1007/BF02836430

Huang Dong-mei, Huang Guang-ying, Lu Fu-er. Effect of Bushen Yiqi Hexue recipe on pinopodes expression on endometrial surface in embryo implantation dysfunctional mice. [J]. Chinese Journal of Integrative Medicine 10(4):279-283(2004)
Dong-mei, H., Guang-ying, H. & Fu-er, L. Effect of Bushen Yiqi Hexue recipe on pinopodes expression on endometrial surface in embryo implantation dysfunctional mice., Chin. J. Integr. Med. 10, 279–283 (2004). https://doi.org/10.1007/BF02836430 DOI：

Huang Dong-mei, Huang Guang-ying, Lu Fu-er. Effect of Bushen Yiqi Hexue recipe on pinopodes expression on endometrial surface in embryo implantation dysfunctional mice. [J]. Chinese Journal of Integrative Medicine 10(4):279-283(2004) DOI： 10.1007/BF02836430.

摘要

Objective: To explore the therapeutic mechanism of Bushen Yiqi Hexue recipe (BYHR)

and observe its effect on pinopodes expression on endometrial surface in mifepristone induced embryo implantation dysfunctional mice.Methods: Pregnant Kunming mice were randomly divided into the normal group

the control group and the treated group. Mice in the treated group were administered with BYHR

and those in the normal group and the control group were administered with normal saline

starting from the very first day of pregnancy (Pd1). On the Pd4

mifepristone (RU486) was subcutaneously injected into mice in the control group and the treated group. Pinopodes expression on endometrial surface at 09:30–10:00 pm on Pd4 (regarded as time point 1

T1) and at 09:30–10:00 am on Pd5 (as time point 2

T2) was determined by scanning electron microscopy. Pregnancy rate and average implanted embryos were observed on Pd7.Results: Pregnancy rate and average implanted embryos in the control group were obviously lower than those in the normal group (allP<0.01)

while the two parameters were significantly higher in the treated group (P<0.05

P<0.01

respectively) than those in the control group. In the normal group

abundant developing pinopodes were distributed over the whole endometrial surface in T1

and they were altered to a great deal of fully developed pinopodes in T2. But in the control group

only a few pinopodes were expressed locally on endometrial surface in T1

showing a nonsynchronous figure of development

and they completely disappeared in T2. In the treated group

a lot of developing pinopodes were expressed like those in the normal group but somewhat lagged behind

whereas many fully developed pinopodes were expressed in T2.Conclusion: The decrease of the pinopodes in Tl and the cleaning up of them in T2 is possibly the partial mechanism of mifepristone in inducing embryo implantation dysfunction. It is indicated that BYHR could improve pinopodes expression on endometrial surface

eventually better the uterine receptivity and improve the embryo implantation.

Abstract

Objective: To explore the therapeutic mechanism of Bushen Yiqi Hexue recipe (BYHR)

the control group and the treated group. Mice in the treated group were administered with BYHR

and those in the normal group and the control group were administered with normal saline

starting from the very first day of pregnancy (Pd1). On the Pd4

mifepristone (RU486) was subcutaneously injected into mice in the control group and the treated group. Pinopodes expression on endometrial surface at 09:30–10:00 pm on Pd4 (regarded as time point 1

T1) and at 09:30–10:00 am on Pd5 (as time point 2

while the two parameters were significantly higher in the treated group (P<0.05

P<0.01

respectively) than those in the control group. In the normal group

abundant developing pinopodes were distributed over the whole endometrial surface in T1

and they were altered to a great deal of fully developed pinopodes in T2. But in the control group

only a few pinopodes were expressed locally on endometrial surface in T1

showing a nonsynchronous figure of development

and they completely disappeared in T2. In the treated group

a lot of developing pinopodes were expressed like those in the normal group but somewhat lagged behind

eventually better the uterine receptivity and improve the embryo implantation.

关键词

Bushen Yiqi Hexue recipeembryo implantation dysfunctionendometriumpinopode

Keywords

Bushen Yiqi Hexue recipeembryo implantation dysfunctionendometriumpinopode

references

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Stavreus-Evers A, Aghajanova L, Brismar H, et al. Co-expression of heparin-binding epidermal growth factor-like growth factor and pinopodes in human endometrium at the time of implantation. Mol Hum Reprod 2002; 8(8): 765–769.

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Shanghai Medical College of Fudan University

Shanghai Institute of Planned Parenthood Research/WHO Collaborating Center for Research in Human Reproduction

Institute of Integrative Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology

Institute of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology

Institute of Reproductive Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology

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