The Property of Nao Yi-an granule in increasing Nestin immunoreactive neural stem cells in rat brains with intracerebral hemorrhage

Tang Tao; Li Xing-qun; Wu Heng; Luo Jie-kun; Zhang Hua-xian; Luo Tuan-lian

doi:10.1007/BF02836431

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The Property of Nao Yi-an granule in increasing Nestin immunoreactive neural stem cells in rat brains with intracerebral hemorrhage

Updated：2021-08-27

- The Property of Nao Yi-an granule in increasing Nestin immunoreactive neural stem cells in rat brains with intracerebral hemorrhage
- Chinese Journal of Integrative Medicine Vol. 10, Issue 4, Pages: 284-288(2004)
- Affiliations：
  
  Institute of Integrative Traditional Chinese Medicine & Western Medicine, Xiangya Hospital, Central South University,Changsha
- Author bio：
- Funds：
  
  Project supported by the National Natural Science Foundation of China (No. 30171192).
- DOI：10.1007/BF02836431
  CLC：
- Published：2004，
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Tao, T., Xing-qun, L., Heng, W. et al. The Property of Nao Yi-an granule in increasing Nestin immunoreactive neural stem cells in rat brains with intracerebral hemorrhage., Chin. J. Integr. Med. 10, 284–288 (2004). https://doi.org/10.1007/BF02836431

Tang Tao, Li Xing-qun, Wu Heng, et al. The Property of Nao Yi-an granule in increasing Nestin immunoreactive neural stem cells in rat brains with intracerebral hemorrhage. [J]. Chinese Journal of Integrative Medicine 10(4):284-288(2004)
Tao, T., Xing-qun, L., Heng, W. et al. The Property of Nao Yi-an granule in increasing Nestin immunoreactive neural stem cells in rat brains with intracerebral hemorrhage., Chin. J. Integr. Med. 10, 284–288 (2004). https://doi.org/10.1007/BF02836431 DOI：

Tang Tao, Li Xing-qun, Wu Heng, et al. The Property of Nao Yi-an granule in increasing Nestin immunoreactive neural stem cells in rat brains with intracerebral hemorrhage. [J]. Chinese Journal of Integrative Medicine 10(4):284-288(2004) DOI： 10.1007/BF02836431.

摘要

Objective: To explore changes of neural stem cells (NSCs) following intracerebral hemorrhage (ICH) and the effect of Nao Yi-an granule (NYA) on them

and furthermore

to explore NYA’s therapeutic mechanismMethods: Sixty rats were randomly divided into three groups

the sham operation control group

the ICH group and the NYA-treated group. Except those in the sham operation control group

all other rats were replicated to ICH models by stereotactical injection of collagenase type VU into their globus pallidus. After modeling

the rats in the NYA-treated group were administered with NYA at the dosage of 4.92 g·kg-1·d-1 by gastrogavage

while those in the other two groups

4 ml distilled water were given instead. The behavioral test was used to evaluate neurological deficit and immunohistochemical method was used to detect Nestin expression

the special marker for neural stem cells.Results: The ICH animals showed notable placing deficit in forlimbs from day 1 to day 28 after modeling. The deficit in the NYA-treated group was similar to that of the ICH group

but the former got recovered better than the latter from day 21 to 28

with significant difference shown (P<0.05). Nestin-positive cells (N+ cells) could be seen around the hemotoma from day 2 on in the ICH group

the number of which gradually increased from day 4 to 7 (P< 0.01) and reached the peak on day 14 (P<0.05)

but reduced significantly on day 28 (P<0.01). Compared with that of the ICH group at the same time points

there was no significant difference in number of N+ cells in the NYA group on day 2 and 4

but it reached the peak earlier on day 7 (P<0.01)

with the level significantly higher than that in the ICH group (P<0.01)

and this high level lasted to day 14.Conclusion: NYA could promote the recovery of behavioral deficit in collagenase type VII-induced ICH rats

and increase the number of nestin-immunoreactive NSCs

suggesting that its effect on NSCs may be one of its pharmaceutical mechanisms in treating ICH.

Abstract

Objective: To explore changes of neural stem cells (NSCs) following intracerebral hemorrhage (ICH) and the effect of Nao Yi-an granule (NYA) on them

and furthermore

to explore NYA’s therapeutic mechanismMethods: Sixty rats were randomly divided into three groups

the sham operation control group

the ICH group and the NYA-treated group. Except those in the sham operation control group

all other rats were replicated to ICH models by stereotactical injection of collagenase type VU into their globus pallidus. After modeling

the rats in the NYA-treated group were administered with NYA at the dosage of 4.92 g·kg-1·d-1 by gastrogavage

while those in the other two groups

4 ml distilled water were given instead. The behavioral test was used to evaluate neurological deficit and immunohistochemical method was used to detect Nestin expression

but the former got recovered better than the latter from day 21 to 28

with significant difference shown (P<0.05). Nestin-positive cells (N+ cells) could be seen around the hemotoma from day 2 on in the ICH group

the number of which gradually increased from day 4 to 7 (P< 0.01) and reached the peak on day 14 (P<0.05)

but reduced significantly on day 28 (P<0.01). Compared with that of the ICH group at the same time points

there was no significant difference in number of N+ cells in the NYA group on day 2 and 4

but it reached the peak earlier on day 7 (P<0.01)

with the level significantly higher than that in the ICH group (P<0.01)

and this high level lasted to day 14.Conclusion: NYA could promote the recovery of behavioral deficit in collagenase type VII-induced ICH rats

and increase the number of nestin-immunoreactive NSCs

suggesting that its effect on NSCs may be one of its pharmaceutical mechanisms in treating ICH.

关键词

Nao Yi-an granuleneural stem cellintracerebral hemorrhagerats

Keywords

Nao Yi-an granuleneural stem cellintracerebral hemorrhagerats

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National Integrative Center for Cardiovascular Diseases, China-Japan Friendship Hospital

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