Effect of Danshao Huaxian capsule on the expression of collagen I, III, and caspase-3 in hepatic fibrosis rats

Geng Xiao-xia; Yang Qin; Xie Ru-jia; Luo Xin-hua; Li Cheng-xiu; Cheng Ming-liang

doi:10.1007/BF02836432

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Effect of Danshao Huaxian capsule on the expression of collagen I, III, and caspase-3 in hepatic fibrosis rats

Updated：2021-08-27

- Effect of Danshao Huaxian capsule on the expression of collagen I, III, and caspase-3 in hepatic fibrosis rats
- Chinese Journal of Integrative Medicine Vol. 10, Issue 4, Pages: 289-295(2004)
- Affiliations：
  
  1. Department of Infectious Diseases, Affiliated Hospital of Guiyang Medical College,Guizhou
  2. Department of Pathophisiology, Guiyang Medical College,China
  3. Department of Infectious Diseases, People’s Hospital of Guizhou Province,China
  4. Department of Pharmacology, Guiyang Medical College,China
- Author bio：
- Funds：
  
  Project supported by the Funds of High-Level Talents of Guizhou Province (No. f03-1)
- DOI：10.1007/BF02836432
  CLC：
- Published：2004，
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Xiao-xia, G., Qin, Y., Ru-jia, X. et al. Effect of Danshao Huaxian capsule on the expression of collagen I, III, and caspase-3 in hepatic fibrosis rats., Chin. J. Integr. Med. 10, 289–295 (2004). https://doi.org/10.1007/BF02836432

Geng Xiao-xia, Yang Qin, Xie Ru-jia, et al. Effect of Danshao Huaxian capsule on the expression of collagen I, III, and caspase-3 in hepatic fibrosis rats. [J]. Chinese Journal of Integrative Medicine 10(4):289-295(2004)
Xiao-xia, G., Qin, Y., Ru-jia, X. et al. Effect of Danshao Huaxian capsule on the expression of collagen I, III, and caspase-3 in hepatic fibrosis rats., Chin. J. Integr. Med. 10, 289–295 (2004). https://doi.org/10.1007/BF02836432 DOI：

Geng Xiao-xia, Yang Qin, Xie Ru-jia, et al. Effect of Danshao Huaxian capsule on the expression of collagen I, III, and caspase-3 in hepatic fibrosis rats. [J]. Chinese Journal of Integrative Medicine 10(4):289-295(2004) DOI： 10.1007/BF02836432.

摘要

Objective: To study the effect of Danshao Huaxian capsule (DSHX)

a traditional Chinese medical prescription

on the expression of collagen (Col) I

III and cysteinyl aspartate specific proteases-3 (caspase-3) in CCl4-induced hepatic fibrosis in rats. And also it is to explore the mechanism of DSHX in anti-fibrosis.Methods: Eighty male Wistar rats were randomly divided into the normal control group (A)

the model group (B)

the un-treated model group (C)

the low-dose-DSHX treated group (D) and the high-dose-DSHX treated group (E). Except those in Group A

all the other rats were made into hepatic fibrotic models by comprehensive processes including subcutaneous injecting of CCl4

and feeding them with alcoholic high-fat and low-protein diet for 8 weeks. Then the two DSHX-treated groups were treated respectively with low dose (0.5 g/kg) and high dose (1.0 g/kg) DSHX capsule by gastrogavage everyday for 8 weeks. At the end of the experiment

the liver index

levels of hyaluronic acid (HA) and alanine aminotransferase (ALT) in serum

degree of hepatic fibrosis

and urinary excretion of hydroxyproline (Hyp) were measured

and the expression of Col I

Col III and caspase-3 in liver tissues were detected respectively by immunohistochemistric technique.Results: Compared with those in Group B and C

the two DSHX treated groups showed that the liver index

levels of serum HA and ALT and severity of hepatic fibrosis were all significantly lower

the urinary excretion of Hyp was significantly higher; the Col I and Col-III expression was lower (Col I: 1.23±1.14

1.07 ±0.96 vs 4.18±2.26

3.22±1.44

P<0.01;Col III: 1.31±0.69

1.09±0.58 vs 3.04±0.62

2.23±0.58

P<0.05). At the same time

the expression of caspase-3 in Group E was fewer than Group B and C in hepatocytes (3.09±0.65 vs 9.60±2.32

8.82±1.45

P<0.01)

but it was extensively expressed in fibrous septal cells(4.52±0.87 vs 1.69±0. 23

2. 98±0. 36

P<0.01).Conclusion: DSHX capsule shows certain therapeutic effect on hepatic fibrosis in rats

and the mechanism might be related with reducing Col I and Col III deposition

inhibiting hepatocyte apoptosis and promoting fibrous septal cells (mainly the activated hepatic stellate cells) apoptosis.

Abstract

Objective: To study the effect of Danshao Huaxian capsule (DSHX)

a traditional Chinese medical prescription

on the expression of collagen (Col) I

the model group (B)

the un-treated model group (C)

the low-dose-DSHX treated group (D) and the high-dose-DSHX treated group (E). Except those in Group A

all the other rats were made into hepatic fibrotic models by comprehensive processes including subcutaneous injecting of CCl4

the liver index

levels of hyaluronic acid (HA) and alanine aminotransferase (ALT) in serum

degree of hepatic fibrosis

and urinary excretion of hydroxyproline (Hyp) were measured

and the expression of Col I

Col III and caspase-3 in liver tissues were detected respectively by immunohistochemistric technique.Results: Compared with those in Group B and C

the two DSHX treated groups showed that the liver index

levels of serum HA and ALT and severity of hepatic fibrosis were all significantly lower

the urinary excretion of Hyp was significantly higher; the Col I and Col-III expression was lower (Col I: 1.23±1.14

1.07 ±0.96 vs 4.18±2.26

3.22±1.44

P<0.01;Col III: 1.31±0.69

1.09±0.58 vs 3.04±0.62

2.23±0.58

P<0.05). At the same time

the expression of caspase-3 in Group E was fewer than Group B and C in hepatocytes (3.09±0.65 vs 9.60±2.32

8.82±1.45

P<0.01)

but it was extensively expressed in fibrous septal cells(4.52±0.87 vs 1.69±0. 23

2. 98±0. 36

P<0.01).Conclusion: DSHX capsule shows certain therapeutic effect on hepatic fibrosis in rats

and the mechanism might be related with reducing Col I and Col III deposition

inhibiting hepatocyte apoptosis and promoting fibrous septal cells (mainly the activated hepatic stellate cells) apoptosis.

关键词

Liver Fibrosiscollagencysteinyl aspartate specific proteases-3apoptosistraditional Chinese herbs

Keywords

Liver Fibrosiscollagencysteinyl aspartate specific proteases-3apoptosistraditional Chinese herbs

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