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Comparative study of the effect of Shugan Shuru Granule on pathology and p53 gene expression in patients with hyperplastic disease of breast
- Chinese Journal of Integrative Medicine Vol. 11, Issue 2, Pages: 123-127(2005)
- Affiliations：
1. Department of Pathology & Pathophysiology, School of Medicine and Life Sciences, Jianghan University,Wuhan
2. Department of Pathology of Wuhan No. 4 Hospital, Jianghan University,China
- Author bio：
- Funds：
DOI：10.1007/BF02836468
CLC：Published：2005，
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Cheng-xin, W., Xuan, H., Xiao-ling, Z. et al. Comparative study of the effect of Shugan Shuru Granule on pathology and p53 gene expression in patients with hyperplastic disease of breast., Chin. J. Integr. Med. 11, 123–127 (2005). https://doi.org/10.1007/BF02836468
Wang Cheng-xin, Huang Xuan, Zhao Xiao-ling, et al. Comparative study of the effect of Shugan Shuru Granule on pathology and p53 gene expression in patients with hyperplastic disease of breast. [J]. Chinese Journal of Integrative Medicine 11(2):123-127(2005)
Cheng-xin, W., Xuan, H., Xiao-ling, Z. et al. Comparative study of the effect of Shugan Shuru Granule on pathology and p53 gene expression in patients with hyperplastic disease of breast., Chin. J. Integr. Med. 11, 123–127 (2005). https://doi.org/10.1007/BF02836468 DOI：
Wang Cheng-xin, Huang Xuan, Zhao Xiao-ling, et al. Comparative study of the effect of Shugan Shuru Granule on pathology and p53 gene expression in patients with hyperplastic disease of breast. [J]. Chinese Journal of Integrative Medicine 11(2):123-127(2005) DOI： 10.1007/BF02836468.
摘要
Objective: To study the effect of Shugan Shuru Granule (SSG) on the p53 gene expression in patients with hyperplastic disease of breast (HDB) to indirectly explore the mechanism of SSG’s effect on HDB on the molecular pathological level.Methods: Sixty-six patients with HDB were allocated in the treated group and the control group
with the former treated with SSG and the latter not. All patients underwent breast operation and their diseased mammary tissues were cut out
sectioned
and observed under microscopy with HE staining and immunohistochemical staining
with ABC method adopted to estimate the degree of hyperplasia and p53 gene expression. The severity of HDB was classified into normal
mild
moderate and severe grades (marked as 0 to III)
according to the degree of hyperplasia in the mammary gland.Results: Hyperplasia in the control group mostly belonged to grade I – III before treatment
showing overgrowth of gland and proliferation of glandular epithelial cells
which were high columnar shaped
more stratified
with papillary or substantive dysplasia. While in the treated group
most belonged to grade 0 – I after SSG treatment
with proliferated gland and dysplasia recovered to normal or disappeared. The positive rate of p53 gene expression in the treated group was 9.09%
and in the control group 39. 39%
comparison between the two groups showing significant difference (P<0. 01)
the intensity in the former was significantly weaker than that in the latter.Conclusion: SSG could not only inhibit the proliferation of mammary duct epithelia and lobuli
but also inhibit the over-expression of P53. Therefore
it could be regarded as an effective remedy for treatment of HDB and prevention of mammary cancer genesis.
Abstract
Objective: To study the effect of Shugan Shuru Granule (SSG) on the p53 gene expression in patients with hyperplastic disease of breast (HDB) to indirectly explore the mechanism of SSG’s effect on HDB on the molecular pathological level.Methods: Sixty-six patients with HDB were allocated in the treated group and the control group
with the former treated with SSG and the latter not. All patients underwent breast operation and their diseased mammary tissues were cut out
sectioned
and observed under microscopy with HE staining and immunohistochemical staining
with ABC method adopted to estimate the degree of hyperplasia and p53 gene expression. The severity of HDB was classified into normal
mild
moderate and severe grades (marked as 0 to III)
according to the degree of hyperplasia in the mammary gland.Results: Hyperplasia in the control group mostly belonged to grade I – III before treatment
showing overgrowth of gland and proliferation of glandular epithelial cells
which were high columnar shaped
more stratified
with papillary or substantive dysplasia. While in the treated group
most belonged to grade 0 – I after SSG treatment
with proliferated gland and dysplasia recovered to normal or disappeared. The positive rate of p53 gene expression in the treated group was 9.09%
and in the control group 39. 39%
comparison between the two groups showing significant difference (P<0. 01)
the intensity in the former was significantly weaker than that in the latter.Conclusion: SSG could not only inhibit the proliferation of mammary duct epithelia and lobuli
but also inhibit the over-expression of P53. Therefore
it could be regarded as an effective remedy for treatment of HDB and prevention of mammary cancer genesis.
关键词
Shugan Shuru Granulehyperplastic disease of breastp53 gene
Keywords
Shugan Shuru Granulehyperplastic disease of breastp53 gene
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