Effects of Shenggu injection on mRNA expression of vascular endothelia growth factor in rat osteoblastsin vitro

Cheng Jie; Peng Rui; Yu Yuan; Wang Yong-qing; Zou Yang; Xia Ren-yun

doi:10.1007/BF02836503

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Effects of Shenggu injection on mRNA expression of vascular endothelia growth factor in rat osteoblastsin vitro

Updated：2021-08-27

- Effects of Shenggu injection on mRNA expression of vascular endothelia growth factor in rat osteoblastsin vitro
- Chinese Journal of Integrative Medicine Vol. 11, Issue 3, Pages: 191-194(2005)
- Affiliations：
  
  1. Department of Orthopaedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan
  2. Department of Orthopaedics, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology,China
  3. Hubei Academy of Traditional Medical and Pharmaceutical Science,China
- Author bio：
- Funds：
  
  Supported by the Project of Health Office of Hubei Province (No. 2003-89-15)
- DOI：10.1007/BF02836503
  CLC：
- Published：2005，
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Jie, C., Rui, P., Yuan, Y. et al. Effects of Shenggu injection on mRNA expression of vascular endothelia growth factor in rat osteoblastsin vitro, ., Chin. J. Integr. Med. 11, 191–194 (2005). https://doi.org/10.1007/BF02836503

Cheng Jie, Peng Rui, Yu Yuan, et al. Effects of Shenggu injection on mRNA expression of vascular endothelia growth factor in rat osteoblastsin vitro. [J]. Chinese Journal of Integrative Medicine 11(3):191-194(2005)
Jie, C., Rui, P., Yuan, Y. et al. Effects of Shenggu injection on mRNA expression of vascular endothelia growth factor in rat osteoblastsin vitro, ., Chin. J. Integr. Med. 11, 191–194 (2005). https://doi.org/10.1007/BF02836503 DOI：

Cheng Jie, Peng Rui, Yu Yuan, et al. Effects of Shenggu injection on mRNA expression of vascular endothelia growth factor in rat osteoblastsin vitro. [J]. Chinese Journal of Integrative Medicine 11(3):191-194(2005) DOI： 10.1007/BF02836503.

摘要

Objective: To study the effects of Shenggu injection (SGI) on mRNA expression of vascular endothelia growth factor (VEGF) in rat osteoblastsin vitro and to explore its possible molecular mechanisms in promoting fracture healing.Methods: Rat osteoblasts culturedin vitro were stimulated with SGI according to the protocol. The expression levels of VEGF mRNA in the cells in every group were examined by reverse-transcriptase ploymerase chain reaction (RT-PCR).Results: When osteoblasts were stimulated with different concentrations of SGI for 5 days

the expression of VEGF mRNA peaked with 1 mg/ml SGI on the 5th day. When treated with 1 mg/ml SGI from the 1st to the 5th day

the expression of VEGF mRNA increased gradually with the increase of culturing time.Conclusion: SGI could promote significantly the expression of VEGF mRNA in rat osteoblastsin vitro. The levels of expression of VEGF mRNA changed along with different concentrations and stimulating time of SGI.

Abstract

the expression of VEGF mRNA peaked with 1 mg/ml SGI on the 5th day. When treated with 1 mg/ml SGI from the 1st to the 5th day

关键词

Shenggu injectionosteoblastvascular endothelia growth factorreverse-transcriptase ploymerase chain reactionfracture healing

Keywords

Shenggu injectionosteoblastvascular endothelia growth factorreverse-transcriptase ploymerase chain reactionfracture healing

references

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Saadeh PB, Mehrara BJ, Steinbrech DS, et al. Transforming growth factor-beta 1 modulated the expression of vascular endothelial growth factor by osteoblasts. Am J Physiol 1999;277(4): 628–637.

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