Effects of serum containing Xinlikang on angiotensin II induced hypertrophy in cultured neonatal rat cardiomyocytes

Tu Xin; Wang Ying; Tu Jin-wen; Yang Yi; Wang Jin-ming

doi:10.1007/BF02836521

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Effects of serum containing Xinlikang on angiotensin II induced hypertrophy in cultured neonatal rat cardiomyocytes

Updated：2021-08-27

- Effects of serum containing Xinlikang on angiotensin II induced hypertrophy in cultured neonatal rat cardiomyocytes
- Chinese Journal of Integrative Medicine Vol. 12, Issue 3, Pages: 194-198(2006)
- Affiliations：
  
  1. Center for Human Genome Research, College of Life Science and Technology, Huazhong University of Science andTechnology,Wuhan
  2. Department of Cardiology, 1st Hospital, Zhengzhou University,China
  3. Department of Internal Medicine, Hubei Hospital of Traditional Chinese Medicine, Hubei College of Traditional Chinese Medicine,China
  4. Departmentof Cardiology, Renmin Hospital, Wuhan University,China
- Author bio：
- Funds：
  
  Supported by Grant from Hubei Science and Technology Bureau Research Project (No. 2001AA309B)
- DOI：10.1007/BF02836521
  CLC：
- Published：2006，
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Xin, T., Ying, W., Jin-wen, T. et al. Effects of serum containing Xinlikang on angiotensin II induced hypertrophy in cultured neonatal rat cardiomyocytes., Chin. J. Integr. Med. 12, 194–198 (2006). https://doi.org/10.1007/BF02836521

Tu Xin, Wang Ying, Tu Jin-wen, et al. Effects of serum containing Xinlikang on angiotensin II induced hypertrophy in cultured neonatal rat cardiomyocytes. [J]. Chinese Journal of Integrative Medicine 12(3):194-198(2006)
Xin, T., Ying, W., Jin-wen, T. et al. Effects of serum containing Xinlikang on angiotensin II induced hypertrophy in cultured neonatal rat cardiomyocytes., Chin. J. Integr. Med. 12, 194–198 (2006). https://doi.org/10.1007/BF02836521 DOI：

Tu Xin, Wang Ying, Tu Jin-wen, et al. Effects of serum containing Xinlikang on angiotensin II induced hypertrophy in cultured neonatal rat cardiomyocytes. [J]. Chinese Journal of Integrative Medicine 12(3):194-198(2006) DOI： 10.1007/BF02836521.

摘要

Objective: To evaluate the effects of Xinlikang (XLK) on angiotensin II (Ang II) induced hypertrophic cultured neonatal rat’s cardiomyocyte (CMC).Methods: Primary cultured neonatal rat’s CMCs with the purity certified by immunohistochemical technique

were divided into three groups. Rats in the normal control group were untreated;those in the model group were established into hypertrophic models but underwent no treatment;and those in the XLK group were established to hypertrophic models and treated with XLK containing serum obtained from rats with aorta coarctation after 8 days of feeding with XLK. MTT and phase-contrast microscope were used to evaluate the effect of XLK on cell activity

pulsating rhythm and surface area;Atrial natriuretic peptide (ANP) expression was determined by radioimmunoassay; Protein content was determined by Bradford method;and DNA synthesis was detected by flow cytometric assay.Results: Immunohistochemistry results showed that more than 90% of the cells were α-sarcometin actin stained positive cells. No significant effect of XLK on normal CMC was found. Ang II could significantly induce hypertrophy in CMCs

and XLK could significantly decrease the increased surface area and the accelerated pulsating rate in them. ANP expression was 780 ±38 μg/L in the model group

and 430 ±23 ώg/L in the control group

and the elevated expression of ANP in model rats was significantly decreased in the XLK group;The DNA content in the G0/G1 and G2/M phases was significantly enhanced and at the same time i was accompanied with increase of total protein content in the model rats after being stimulated by Ang II for 24 h

showing that serum-containing XLK could also significantly suppress total protein synthesis (P < 0. 05).Conclusion: XLK could improve Ang II mediated pathological growth of CMCs without influencing the growth of normal CMCs

suggesting that XLK is probably an effective drug for treatment of myocardial hypertrophy and heart failure.

Abstract

and XLK could significantly decrease the increased surface area and the accelerated pulsating rate in them. ANP expression was 780 ±38 μg/L in the model group

and 430 ±23 ώg/L in the control group

suggesting that XLK is probably an effective drug for treatment of myocardial hypertrophy and heart failure.

关键词

Xinlikangangiotensin IIcardiomyocytehypertrophy

Keywords

Xinlikangangiotensin IIcardiomyocytehypertrophy

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Key Laboratory of Basic Pharmacology of Guizhou, Department of Pharmacology, Zunyi Medical College

Department of Hematology, the Affiliated Hospital of Guiyang Medical College

Key Laboratory of Basic Pharmacology of Guizhou and Department of Pharmacology, Zunyi Medical College

The Experimental Research Center, China Academy of Chinese Medical Sciences

The Cardiology Department of Chongqing Hospital of Traditional Chinese Medicine and Chongqing the First Hospital

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