Study on effects of extracts fromSalvia Miltiorrhiza andCurcuma Longa in inhibiting phosphorylated extracellular signal regulated kinase expression in rat’s hepatic stellate cells

Cheng Yang; Ping Jian; Liu Cheng; Tan Ying-zi; Chen Gao-feng

doi:10.1007/BF02836524

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Study on effects of extracts fromSalvia Miltiorrhiza andCurcuma Longa in inhibiting phosphorylated extracellular signal regulated kinase expression in rat’s hepatic stellate cells

Updated：2021-08-27

- Study on effects of extracts fromSalvia Miltiorrhiza andCurcuma Longa in inhibiting phosphorylated extracellular signal regulated kinase expression in rat’s hepatic stellate cells
- Chinese Journal of Integrative Medicine Vol. 12, Issue 3, Pages: 207-211(2006)
- Affiliations：
  
  Department of Hepatocirrhosis, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine,Shanghai
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30300458)
- DOI：10.1007/BF02836524
  CLC：
- Published：2006，
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Yang, C., Jian, P., Cheng, L. et al. Study on effects of extracts fromSalvia Miltiorrhiza andCurcuma Longa in inhibiting phosphorylated extracellular signal regulated kinase expression in rat’s hepatic stellate cells., Chin. J. Integr. Med. 12, 207–211 (2006). https://doi.org/10.1007/BF02836524

Cheng Yang, Ping Jian, Liu Cheng, et al. Study on effects of extracts fromSalvia Miltiorrhiza andCurcuma Longa in inhibiting phosphorylated extracellular signal regulated kinase expression in rat’s hepatic stellate cells. [J]. Chinese Journal of Integrative Medicine 12(3):207-211(2006)
Yang, C., Jian, P., Cheng, L. et al. Study on effects of extracts fromSalvia Miltiorrhiza andCurcuma Longa in inhibiting phosphorylated extracellular signal regulated kinase expression in rat’s hepatic stellate cells., Chin. J. Integr. Med. 12, 207–211 (2006). https://doi.org/10.1007/BF02836524 DOI：

Cheng Yang, Ping Jian, Liu Cheng, et al. Study on effects of extracts fromSalvia Miltiorrhiza andCurcuma Longa in inhibiting phosphorylated extracellular signal regulated kinase expression in rat’s hepatic stellate cells. [J]. Chinese Journal of Integrative Medicine 12(3):207-211(2006) DOI： 10.1007/BF02836524.

摘要

Objective: To study the effect of salvianolic acid B (SAB) and curcumin

the extracts ofSalvia Miltiorrhiza andCurcuma Longa

on the proliferation and activation of hepatic stellate cell (HSC)

and the extracellular signal regulated kinase (ERK) expression in it.Methods: Rat’s HSC-T6 were cultured and treated by SAB or curcumin. The inhibitory effect on cell proliferation was determined by 3-(4

5-dimthyl-2-2-thiazoly)-2

5-diphenyl-2H-tetrazolium bromide (MTT) colorimetry

and the expression levels of α smooth actin (α-SMA)

collagen type I

and ERK were determined by Western blot.Results: SAB and curcumin inhibited the proliferation and activation of rat’s HSC-T6 in dose-dependent fashion and significantly reduced the expression level of a-SMA (P < 0.01). Curcumin significantly reduced the expression of collagen type I (P < 0.05). Both SAB and curcumin showed insignificant effect on the ERK expression level

but they could significantly reduce the level of phosphorylated-ERK expression

showing significant difference as compared with that in the control group (P < 0. 01 andP < 0. 05 respectively).Conclusion: SAB and curcumin could significantly inhibit the proliferation

activation of HSC

and the production of type I collagen in HSC

the mechanism may be associated with their inhibition on ERK phosphorylation.

Abstract

Objective: To study the effect of salvianolic acid B (SAB) and curcumin

the extracts ofSalvia Miltiorrhiza andCurcuma Longa

on the proliferation and activation of hepatic stellate cell (HSC)

5-dimthyl-2-2-thiazoly)-2

5-diphenyl-2H-tetrazolium bromide (MTT) colorimetry

and the expression levels of α smooth actin (α-SMA)

collagen type I

but they could significantly reduce the level of phosphorylated-ERK expression

showing significant difference as compared with that in the control group (P < 0. 01 andP < 0. 05 respectively).Conclusion: SAB and curcumin could significantly inhibit the proliferation

activation of HSC

and the production of type I collagen in HSC

the mechanism may be associated with their inhibition on ERK phosphorylation.

关键词

salvianolic acid BCurcuminextracellular signal regulated kinasephosphorylation

Keywords

salvianolic acid BCurcuminextracellular signal regulated kinasephosphorylation

references

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