Effects of baicalin on expression of inducible nitric oxide synthase in cultured fibroblasts stimulated by cytokines

Bi Xin-ling; Gu Jun; Nie Ben-yong; Li Quan; Liu Hui; Mi Qing-sheng

doi:10.1007/BF02836558

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Effects of baicalin on expression of inducible nitric oxide synthase in cultured fibroblasts stimulated by cytokines

Updated：2021-08-27

- Effects of baicalin on expression of inducible nitric oxide synthase in cultured fibroblasts stimulated by cytokines
- Chinese Journal of Integrative Medicine Vol. 10, Issue 1, Pages: 40-43(2004)
- Affiliations：
  
  1. Department of Dermatology, Changhai Hospital of the Second Military Medical University,Shanghai
  2. Department of Dermatology, Military Hospital210, Dalian City,Liaoning Province
  3. John Robarts Institute, West Ontario University,Canada
- Author bio：
- Funds：
  
  This Project is supported by State Natural Science Foundation (No. 30271197)
- DOI：10.1007/BF02836558
  CLC：
- Published：2004，
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Xin-ling, B., Jun, G., Ben-yong, N. et al. Effects of baicalin on expression of inducible nitric oxide synthase in cultured fibroblasts stimulated by cytokines., Chin. J. Integr. Med. 10, 40–43 (2004). https://doi.org/10.1007/BF02836558

Bi Xin-ling, Gu Jun, Nie Ben-yong, et al. Effects of baicalin on expression of inducible nitric oxide synthase in cultured fibroblasts stimulated by cytokines. [J]. Chinese Journal of Integrative Medicine 10(1):40-43(2004)
Xin-ling, B., Jun, G., Ben-yong, N. et al. Effects of baicalin on expression of inducible nitric oxide synthase in cultured fibroblasts stimulated by cytokines., Chin. J. Integr. Med. 10, 40–43 (2004). https://doi.org/10.1007/BF02836558 DOI：

Bi Xin-ling, Gu Jun, Nie Ben-yong, et al. Effects of baicalin on expression of inducible nitric oxide synthase in cultured fibroblasts stimulated by cytokines. [J]. Chinese Journal of Integrative Medicine 10(1):40-43(2004) DOI： 10.1007/BF02836558.

摘要

Objective: To investigate the effects of baicalin on expression of inducible nitric oxide synthase (iNOS) in fibroblasts and its mechanisms in treating psoriasis.Methods: Fibroblasts cultured in vitro were stimulated with tumor necrosis factor-α (TNF-α)

interferon-γ (IFN-γ)

interleukin-8 (IL-8) in different groups. iNOS was detected by western blot and immunocytochemistry assay

and in addition

the effects of baicalin on its expression were investigated.Results: Fibroblasts did not express iNOS without cytokine stimulation. When treated for 24 h with 1.0 × 106U/L TNF-α

0.2 × 106U/L IFN-γ

0.2 × 106 pg/L IL-8 alone or in combinations indicated

fibroblasts produced iNOS when stimulated by TNF-α alone while neither IFN-γ nor IL-8 could induce the production of iNOS. The combination of TNF-α and IL-8 induced a strong expression of iNOS

the combined exposure of three kinds of cytokines showed an even stronger effects. The strongly stained area was in the cytoplasm near the nuclei. Expression of iNOS induced by TNF-α and IL-8 was inhibited by 50 μg/ ml of baicalin.Conclusion: Fibroblasts might express iNOS when stimulated by certain cytokines. Baicalin decreased production of nitric oxide through inhibiting the expression of iNOS

furthermore it reduced inflammation

which might be part of its mechanisms in treating psoriasis.

Abstract

interferon-γ (IFN-γ)

interleukin-8 (IL-8) in different groups. iNOS was detected by western blot and immunocytochemistry assay

and in addition

the effects of baicalin on its expression were investigated.Results: Fibroblasts did not express iNOS without cytokine stimulation. When treated for 24 h with 1.0 × 106U/L TNF-α

0.2 × 106U/L IFN-γ

0.2 × 106 pg/L IL-8 alone or in combinations indicated

fibroblasts produced iNOS when stimulated by TNF-α alone while neither IFN-γ nor IL-8 could induce the production of iNOS. The combination of TNF-α and IL-8 induced a strong expression of iNOS

furthermore it reduced inflammation

which might be part of its mechanisms in treating psoriasis.

关键词

Baicalinfibroblastsinducible nitric oxide synthase

Keywords

Baicalinfibroblastsinducible nitric oxide synthase

references

Werner S, Smole H. Paracrine regulation of keratinocyte proliferation and differentiation. Trends Cell Biol 2001; 11: 143–146.

Fransson J, de la Torre B, Hammar H. Psoriatic fibroblasts secrete lower amounts of IL-6 than healthy fibroblasts before and after stimulation with TNF-α. Arch Dermatol Res 1999;291: 538–541.

Shimizu Y, Sakai M, Umemura Y, et al. Immunohistochemical localization of nitric oxide synthase in normal human skin: expression of endothelial-type and inducibletype nitric oxide synthase in keratinocytes. J Dermatol 1997;24: 80–87.

Zheng MR, Xie Y, Sun RZ, et al. Report on the treatment of psoriasis vulgaris by baicalin. Chin J Derm Venereol 1990;4: 217–218.

Cavicchi M, Whittle BJ. Potentiation of cytokine induced iNOS expression in the human intestinal epithelial cell line, DLD-1 by cyclic AMP. Gut 1999;45: 367–374.

Miura H, Sano S, Higashiyama M, et al. Involvement of insulin-like growth factorI in psoriasis as a paracrine growth factor: dermal fibroblasts play a regulatory role in developing psoriatic lesions. Arch Dermatol Res 2000; 292: 590–597.

Oyama N, Iwatsuki K, Satoh M, et al. Dermal fibroblasts are one of the therapeutic targets for topical application of la, 25-dihydroxyvitamin D3: the possible involvement of transforming growth factor-β induction. Br J Dermatol 2000; 143: 1140–1148.

Qin XW, Zheng MR, Mou XL, et al. Effects of baicalin on the chemotaxis of psoriatic PMN to LTB4. Chin J Dermatol 1998;2: 116–117.

Chen YC, Shen SC, Chen LG, et al. Wogonin, baicalin, and baicalein inhibition of inducible nitric oxide synthase and cyclo-oxygenase-2 gene expressions induced by nitric oxide synthase inhibitors and lipopolysaccharide. Biochem Pharmacol 2001;61: 1417–1427.

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