Effects of Nephritis No. 3 recipe on nitric oxide, nitric oxide synthase secreted by cultured mesangial cells in rats and the gene expression of inducible nitric oxide synthase

Chen Zhi-qiang; Huang Huai-peng; Huang Wen-zheng; Zhu Xiao-di; Lin Qing-qi

doi:10.1007/BF02838036

Your Location：

Home >

Browse articles >

Effects of Nephritis No. 3 recipe on nitric oxide, nitric oxide synthase secreted by cultured mesangial cells in rats and the gene expression of inducible nitric oxide synthase

Updated：2021-08-27

- Effects of Nephritis No. 3 recipe on nitric oxide, nitric oxide synthase secreted by cultured mesangial cells in rats and the gene expression of inducible nitric oxide synthase
- Chinese Journal of Integrative Medicine Vol. 9, Issue 3, Pages: 210-214(2003)
- Affiliations：
  
  1. Institute of Integrative Chinese and Western Medicine, Hebei Medical University,Shijiazhuang
  2. Nanjing University of TCM,China
  3. The Affiliated Hospital of Tianjin College of TCM,China
- Author bio：
- Funds：
  
  The item was supported by Tianjin Scientific Committee (No. 953101311) and won the second grade Science and Technology Progress award of Tianjin in 2001
- DOI：10.1007/BF02838036
  CLC：
- Published：2003，
Scan for full text
Zhi-qiang, C., Huai-peng, H., Wen-zheng, H. et al. Effects of Nephritis No. 3 recipe on nitric oxide, nitric oxide synthase secreted by cultured mesangial cells in rats and the gene expression of inducible nitric oxide synthase., Chin. J. Integr. Med. 9, 210–214 (2003). https://doi.org/10.1007/BF02838036

Chen Zhi-qiang, Huang Huai-peng, Huang Wen-zheng, et al. Effects of Nephritis No. 3 recipe on nitric oxide, nitric oxide synthase secreted by cultured mesangial cells in rats and the gene expression of inducible nitric oxide synthase. [J]. Chinese Journal of Integrative Medicine 9(3):210-214(2003)
Zhi-qiang, C., Huai-peng, H., Wen-zheng, H. et al. Effects of Nephritis No. 3 recipe on nitric oxide, nitric oxide synthase secreted by cultured mesangial cells in rats and the gene expression of inducible nitric oxide synthase., Chin. J. Integr. Med. 9, 210–214 (2003). https://doi.org/10.1007/BF02838036 DOI：

Chen Zhi-qiang, Huang Huai-peng, Huang Wen-zheng, et al. Effects of Nephritis No. 3 recipe on nitric oxide, nitric oxide synthase secreted by cultured mesangial cells in rats and the gene expression of inducible nitric oxide synthase. [J]. Chinese Journal of Integrative Medicine 9(3):210-214(2003) DOI： 10.1007/BF02838036.

摘要

Objective: To explore the effect of the Nephritis No. 3 (N-3) recipe on nitric oxide (NO)

nitric oxide synthase (NOS) secreted by cultured mesangial cells (MC) and its gene expression of the inducible nitric oxide synthase (iNOS).Methods; The drug (nephritis No. 3)-containing serum was prepared with serum pharmacological technique

and then was applied to react on mesangial cells cultured in fetal calf serum (FCS) and cells cultured in FCS plus lipopolysaccharide. To observe the secretion of NO and NOS and the gene expression of iNOS by means of RT-PCR.Results: Under the two kinds of culture conditions

the content of NO and NOS in the groups with drug-containing serum were higher than those without drug-containing serum (P < 0.05

P < 0.01)

and the expression of iNOS mRNA was up-regulated too.Conclusion: The N-3 could significantly promote the secretion of NO and NOS and the mRNA expression of iNOS in rats.

Abstract

Objective: To explore the effect of the Nephritis No. 3 (N-3) recipe on nitric oxide (NO)

the content of NO and NOS in the groups with drug-containing serum were higher than those without drug-containing serum (P < 0.05

P < 0.01)

and the expression of iNOS mRNA was up-regulated too.Conclusion: The N-3 could significantly promote the secretion of NO and NOS and the mRNA expression of iNOS in rats.

关键词

Nephritis No. 3 recipemesangial cellNitric Oxidenitric oxide synthase

Keywords

Nephritis No. 3 recipemesangial cellNitric Oxidenitric oxide synthase

references

WANG DH, XU XM. NO and nephrosclerosis. Foreign Med, Urolog Fascicles 1998; 18(1): 6–8.

LI XM, DAI ZH, WANG HY. Glomerular MC hyperplastic inhibitory factor. Foreign Med, Urolog Fascicles 1997; 17(1): 31–33.

JIANG DX, HUANG WZ, YANG HT, et al. Experimental study of N-3 recipe on glomerular MC proliferation and its generation of TNF-α. Chin J Basic Med 1999; 5(10): 36–39.

CHEN YP, GAO J, XING BC, et al. Culture of glomerular MC. Acad J Beijing Med Univ 1989; 21(4): 335 -336.

HU MC, YU BS. Action of NO in nephropathy. Chin J Nephropathy 1996; 12 (2): 121–123.

CHEN M, HUANG SM. Enthothelin, NO and diabetic nephropathy. Foreign Med, Endocrinology Fascicle 1999; 19(2): 69–72.

Trachtman H, Futteweit S, Singhal PC. Nitric oxide modulates the synthesis of extracellular matrix protein in cultured rat mesangeal cells. Biochem Biophys Res Commun 1995; 270(1): 120–125.

Trachtman H, Futteweit S, Grag P, et al. Nitric oxide stimulates the activity of a 72-Kda neutral matrix metalloproteinase in cultured rat mesangeal cells. Biochem Biophys Res Commun 1996; 218(3): 704–708.

ZHONG CS, SUN AY. Biomedicine of nitric oxide. Shanghai! Shanghai Med Univ Press, 1997: 256–257.

CHEN ZQ, HUANG HP, HUANG WZ, et al. Effect of N-3 recipe on TGF-α 1 of glomerular MC and its mRNA expression. Chin J TCM Basic Med 2002; 8(9): 46 -48.

FULL TEXT LINK

More>

Views

333

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Clinical effect of Fuzheng Jiedu granule on serum NO, NOS and microelements in patients with coronary heart disease in long-term contact with nickel

Effect of Shengmai injection (生脉注射液) on diaphragmatic contractility in doxorubicin-treated rats

Metabolic regulatory and anti-oxidative effects of modified bushen huoxue decoction (补肾活血方) on experimental rabbit model of osteoarthritis

Divergent immunomodulatory effects of extracts and phenolic compounds from the fern Osmunda japonica Thunb.

Related Author

No data

Related Institution

Institute of Integrative Chinese and Western Medicine, Lanzhou Medical College

Department of Cardiovascular Diseases, Workers’ Hospital, Jinchuan Co.

Department of Hematology, the Affiliated Hospital of Guiyang Medical College

Key Laboratory of Basic Pharmacology of Guizhou and Department of Pharmacology, Zunyi Medical College

Department of Physiology, Bengbu Medical College

Address：1 Caochang, xiyuan, Beijing, People's Republic of China Postal code：100091
Tel：010-62886827, 62877592 Fax：010-62874291
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05067934号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰