Role of low dosage arsenic trioxide on pulmonary dendritic cells in asthmatic mice

Zhou Lin-fu; Yin Kai-sheng; Zhou Zhi-min

doi:10.1007/BF02838615

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Role of low dosage arsenic trioxide on pulmonary dendritic cells in asthmatic mice

Updated：2021-08-27

- Role of low dosage arsenic trioxide on pulmonary dendritic cells in asthmatic mice
- Chinese Journal of Integrative Medicine Vol. 9, Issue 4, Pages: 281-284(2003)
- Affiliations：
  
  Department of Respiratory Diseases, the First Affiliated Hospital of Nanjing Medical University,Nanjing
- Author bio：
- Funds：
  
  This study was supported by a research foundation from Jiangsu Provincial Administration Bureau of TCM (No. 9974)
- DOI：10.1007/BF02838615
  CLC：
- Published：2003，
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Lin-fu, Z., Kai-sheng, Y. & Zhi-min, Z. Role of low dosage arsenic trioxide on pulmonary dendritic cells in asthmatic mice., Chin. J. Integr. Med. 9, 281–284 (2003). https://doi.org/10.1007/BF02838615

Zhou Lin-fu, Yin Kai-sheng, Zhou Zhi-min. Role of low dosage arsenic trioxide on pulmonary dendritic cells in asthmatic mice. [J]. Chinese Journal of Integrative Medicine 9(4):281-284(2003)
Lin-fu, Z., Kai-sheng, Y. & Zhi-min, Z. Role of low dosage arsenic trioxide on pulmonary dendritic cells in asthmatic mice., Chin. J. Integr. Med. 9, 281–284 (2003). https://doi.org/10.1007/BF02838615 DOI：

Zhou Lin-fu, Yin Kai-sheng, Zhou Zhi-min. Role of low dosage arsenic trioxide on pulmonary dendritic cells in asthmatic mice. [J]. Chinese Journal of Integrative Medicine 9(4):281-284(2003) DOI： 10.1007/BF02838615.

摘要

Objective: To investigate the distribution and recruitment of pulmonary dendritic cells (DCs) and the influence of low dosage arsenic trioxide (As2O3) on them in the airway of asthmatic mice.Methods: Thirty BALB/c mice were randomly divided into 3 groups: the control group

the asthmatic group and the As2 O3 treated group. The mice asthmatic model was induced via sensitizing with peritoneal injection of ovalbumin (OVA) for two times and then provocated with aerosol inhalation of OVA for a week. The treated group was peritoneally injected with 0. 2 ml solution of As2O3 (4mg/kg) 0. 5h after each provocation. The immunohistochemistry and computerised image analysis were applied to detect quantitatively the DCs in the lung and airway of mice.Results: All intraepithelial nonlymphoid dendritic cells-145 (NLDC-145) throughout the respiratory tree in the mice of the control group formed a network with the density of DCs varying from (575±54) cells/mm2 epithelial surface in the large airway

to (68±12) cells/ mm2 epithelial surface in the small airway. The distribution of airway NLDC-145+ in the asthmatic group was similar to that in the control group

but its density was significantly upregulated (P < 0.01). The distribution of airway NLDC-145 in the treated group was similar to that in the asthmatic group

only its density was significantly downregulated (P < 0.01).Conclusion: There is an integral network of NLDC-145+ throughout the respiratory tree. To downregulate the density but not change the distribution of pulmonary DCs could be an important therapeutic mechanism of low dosage As2 O3 in treating asthma.

Abstract

to (68±12) cells/ mm2 epithelial surface in the small airway. The distribution of airway NLDC-145+ in the asthmatic group was similar to that in the control group

but its density was significantly upregulated (P < 0.01). The distribution of airway NLDC-145 in the treated group was similar to that in the asthmatic group

关键词

Arsenic Trioxidebronchial asthmadendritic cellimmunohistochemistry

Keywords

Arsenic Trioxidebronchial asthmadendritic cellimmunohistochemistry

references

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