Effect of muscone on neuronal glutamate transporter EAAC1 expression in rats with acute cerebral ischemia

Liang Hui; Chen Hu; Gao Yin; Cai Ding-fang

doi:10.1007/BF02838617

Your Location：

Home >

Browse articles >

Effect of muscone on neuronal glutamate transporter EAAC1 expression in rats with acute cerebral ischemia

Updated：2021-08-27

- Effect of muscone on neuronal glutamate transporter EAAC1 expression in rats with acute cerebral ischemia
- Chinese Journal of Integrative Medicine Vol. 9, Issue 4, Pages: 285-288(2003)
- Affiliations：
  
  Department of Integrated TCM and Western Medicine, Huashan Hospital, Fudan University,Shanghai
- Author bio：
- Funds：
  
  This study was supported by the fund of “Hundred Persons Project” of Shanghai Public Health Bureau (No. 97BR016)
- DOI：10.1007/BF02838617
  CLC：
- Published：2003，
Scan for full text
Hui, L., Hu, C., Yin, G. et al. Effect of muscone on neuronal glutamate transporter EAAC1 expression in rats with acute cerebral ischemia., Chin. J. Integr. Med. 9, 285–288 (2003). https://doi.org/10.1007/BF02838617

Liang Hui, Chen Hu, Gao Yin, et al. Effect of muscone on neuronal glutamate transporter EAAC1 expression in rats with acute cerebral ischemia. [J]. Chinese Journal of Integrative Medicine 9(4):285-288(2003)
Hui, L., Hu, C., Yin, G. et al. Effect of muscone on neuronal glutamate transporter EAAC1 expression in rats with acute cerebral ischemia., Chin. J. Integr. Med. 9, 285–288 (2003). https://doi.org/10.1007/BF02838617 DOI：

Liang Hui, Chen Hu, Gao Yin, et al. Effect of muscone on neuronal glutamate transporter EAAC1 expression in rats with acute cerebral ischemia. [J]. Chinese Journal of Integrative Medicine 9(4):285-288(2003) DOI： 10.1007/BF02838617.

摘要

Objective: To investigate muscone’s neuroprotective action and its mechanism in rats with acute cerebral ischemic injury.Methods: Rat focal ischemic model of middle cerebral artery occlusion (MCAO) was established by thread inserting method. TTC staining

RT-PCR and Western blot analysis were used to study the effects of muscone on infracted volume

EAAClmRNA and protein levels.Results: Infarcted volume in the muscone treated group was significantly lower than that in the model control group. Compared with normal group

EAAC1 mRNA level increased in the model control group at 6 and 24 hrs

and protein level increased at 24 hrs after modelling

muscone could reduce the increased expression in model rats.Conclusion: Muscone possesses the action of neuroprotection

its mechanism may be related to reduction of EAAC1 and protein expressions.

Abstract

RT-PCR and Western blot analysis were used to study the effects of muscone on infracted volume

EAAClmRNA and protein levels.Results: Infarcted volume in the muscone treated group was significantly lower than that in the model control group. Compared with normal group

EAAC1 mRNA level increased in the model control group at 6 and 24 hrs

and protein level increased at 24 hrs after modelling

muscone could reduce the increased expression in model rats.Conclusion: Muscone possesses the action of neuroprotection

its mechanism may be related to reduction of EAAC1 and protein expressions.

关键词

musconeCerebral Ischemianeuronal glutamate transporterneuroprotection

Keywords

musconeCerebral Ischemianeuronal glutamate transporterneuroprotection

references

XIA X, ZHANG YX. Mordern application and research of traditional Chinese medicine-section of internal medicine. Shanghai: Shanghai Traditional Chinese Medical University Press, 1997: 407–409.

JIANG ZY, LI CD, ZHOU D etal. Protection effect of moschus on neuron damage caused by rat’s experimental cerebral ischemia. Chinese Journal of Traditional Medical Science and Technology 2001;8(2): 96–97

JI YB. Pharmacological action and application of available composition of traditional Chinese medicine. Haerbing: Heilongjiang Scientific and Technical Press, 1995: 313–315.

Yukio Nishizawa. Glutamate release and neuronal damage in ischemia. Life Science 2001;69(4): 369–381

Danbolt NC. Glutamate uptake. Progress in Neurology 2001;65(1): 1–105.

David JR. Takeo Oshima, David A. Glutamate in severe brain ischemia is mainly by reversed uptake. Nature 2000, 403(6767): 316–321

Longa EZ, Weinstein PR, Carlson S, et al. Reversible middle cerebral artery occlusion without craniectomy in rat. Stroke 1989; 20: 84–91.

Derugin N, Wendland M, Muramatsu K, et al. Evolution of brain injury after transient middle cerebral artery occlusion in neonatal rats. Stroke 2000; 31 (7): 1752–1761.

Li Tao, Daiwei Shen, Sujay Pandey, et al. Structure and developmental expression of the mouse RGR opsin gene. Molecular Vision 1998;4: 25–30.

MO QZ, GONG B, MAO HM, et al. The absorption, distribution and excretion of3H-muscone in the body. Research of Chinese traditional patent medicine 1984; (7): 1–4.

Knai Y, Trotti D, NussbergerS, et al. The high-affinity glutamate transporter family. In: Reith MEA ed. Neurotransmitter Transporters: Structure, Function and regulation. New Jerey: Human Press, 1997: 171–181.

Dube GR, Marshall KC. Modulation of excitatory synaptic transmission in locus coeruleus by multiple presynaptic merabotropic glutamate receptors. Neuroscience 1997;80: 511–521.

FULL TEXT LINK

More>

Views

348

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Baicalin attenuates focal cerebral ischemic reperfusion injury by inhibition of protease-activated receptor-1 and apoptosis

Treatment of cerebral ischemia with combination of bone marrow-derived mesenchymal stem cells and Chinese medicine

Attenuation of brain inflammatory response after focal cerebral ischemia/reperfusion with Xuesaitong Injection in rats

Assessment and explorations on the mechanism of neuroprotection of patients in ischemic stroke by traditional Chinese medicine

Related Author

No data

Related Institution

Department of Carcliology and Neurology, Hospital of Oriental Medicine, Kyung Hee University

Department of Herbal Pharmacology, College of Korean Medicine, Kyung Hee University

Department of Gerontology, Qilu Hospital, Shandong University

China Institute of Pharmacology, College of Medicine, Shandong University

Department of Experimental Pathology, Institute of Basic Medicine, Shandong Academy of Medical Sciences

Address：1 Caochang, xiyuan, Beijing, People's Republic of China Postal code：100091
Tel：010-62886827, 62877592 Fax：010-62874291
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05067934号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰