To evaluate the effect of ligustrazine on bone marrow hematopoietic microenvironment and signal transduction in irradiation injured mice. After being irradiated by 6.0 Gy 60Co Y-ray

the mice in the ligustrazine group were orally given ligustrazine 4 mg/mouse twice a day to the end of the experiment. For the control group

normal saline was given instead of ligustrazine and the mice in the normal group was untreated. The mice were sacrificed separately on the 3rd

7th and 14th day after radiation

bone marrow of them was taken and managed as follows: (1) Make stromal cell culture to observe the growth state and the expression of focal adhesion kinase (FAK) of the cells; (2) Make the bone marrow section to examine the pathological and immunohistochemistry changes

including the expression of fetal liver kinase-1 (Flk-1) and FVIII: RAg. The data were recorded and compared among groups. After radiation

the hematopoietic cells in bone marrow decreased and the micro-vessels dilated and congested with bleeding; bone marrow became thinner

red colored with cells of irregular shape and few cells adhere to the bottom of culture flask. These changes began to recover at the 7th day and approached to normal within 2 weeks. The recovery was better

earlier and quicker in the ligustrazine group than that in the control group. The expression levels of Flk-1 decreased significantly. Ligustrazine could promote the recovery of hematopoietic function of radiation damaged bone marrow

the mechanisms might be through improving the microenvironment and signal transduction path for recovery.