Effects of Astragalus membranaceus in promoting T-helper cell type 1 polarization and interferon-γ production by up-regulating T-bet expression in patients with asthma

Wang Gang; Liu  Chun-tao; Wang Zeng-li; Yan Cun-liang; Luo Feng-ming; Wang Lei; Li Ting-qian

doi:10.1007/s11655-006-0262-y

Your Location：

Home >

Browse articles >

Effects of Astragalus membranaceus in promoting T-helper cell type 1 polarization and interferon-γ production by up-regulating T-bet expression in patients with asthma

Updated：2021-08-27

- Effects of Astragalus membranaceus in promoting T-helper cell type 1 polarization and interferon-γ production by up-regulating T-bet expression in patients with asthma
- Chinese Journal of Integrative Medicine Vol. 12, Issue 4, Pages: 262-267(2006)
- Affiliations：
  
  1. Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University,Chengdu,China
  2. Department of Respiratory Disease, West China Hospital, Sichuan University,China
  3. Department of Laboratory Medicine, Shenzhen Hospital Affiliated to Peking University,China
- Author bio：
- Funds：
  
  Supported by Science Foundation of State Administration of Traditional Chinese Medicine (No. 04-05JQ03), and partly supported by China Postdoctoral Science Foundation (No. 2005037800).
- DOI：10.1007/s11655-006-0262-y
  CLC：
- Published：2006，
Scan for full text
Wang, G., Liu, Ct., Wang, Zl. et al. Effects of Astragalus membranaceus in promoting T-helper cell type 1 polarization and interferon-γ production by up-regulating T-bet expression in patients with asthma., Chin. J Integ. Med. 12, 262–267 (2006). https://doi.org/10.1007/s11655-006-0262-y

Wang Gang, Liu Chun-tao, Wang Zeng-li, et al. Effects of Astragalus membranaceus in promoting T-helper cell type 1 polarization and interferon-γ production by up-regulating T-bet expression in patients with asthma. [J]. Chinese Journal of Integrative Medicine 12(4):262-267(2006)
Wang, G., Liu, Ct., Wang, Zl. et al. Effects of Astragalus membranaceus in promoting T-helper cell type 1 polarization and interferon-γ production by up-regulating T-bet expression in patients with asthma., Chin. J Integ. Med. 12, 262–267 (2006). https://doi.org/10.1007/s11655-006-0262-y DOI：

Wang Gang, Liu Chun-tao, Wang Zeng-li, et al. Effects of Astragalus membranaceus in promoting T-helper cell type 1 polarization and interferon-γ production by up-regulating T-bet expression in patients with asthma. [J]. Chinese Journal of Integrative Medicine 12(4):262-267(2006) DOI： 10.1007/s11655-006-0262-y.

摘要

To explore the effect of Astragalus membranaceus (AM) on T-helper cell type 1 (Th1) specific transcription factor T-box expressed in T cells (T-bet) expression and Th1/Th2 equilibrium. The levels of T-bet mRNA in peripheral blood mononuclear cells (PBMCs) from 15 patients with asthma and 15 healthy subjects were determined by reverse transcription-polymerase chain reaction (RT-PCR). PBMCs in asthma patients were incubated with AM and then the concentration of interferon γ (IFN-γ) and interleukin-4 (IL-4) in the supernate before and after AM intervention were determined by ELISA. The numbers of CD4+CCR3+ and CD4+CCR5+ cells were counted by flow cytometry. The expression of T-bet mRNA and the level of IFN-γ were lower

but level of serum IL-4 was higher in asthma patients when compared with those in healthy subjects respectively. After AM (60 μ g/ml) intervention

the former two parameters raised and showed a positive correlation between them

while the level of IL-4 was decreased. The mean percentage of CD4+CCR3+ cells in asthma patients was significantly higher but that of CD4+CCR5+ cells was lower when compared with those in healthy subjects respectively. After AM intervention

the abnormal change in the two indexes was improved to certain extent

showing a reversing status of Th2 polarization. AM could increase the expression of T-bet mRNA and Th1 cytokines such as IFN-γ

and might reverse the Th2 predominant status in asthma patients.

Abstract

but level of serum IL-4 was higher in asthma patients when compared with those in healthy subjects respectively. After AM (60 μ g/ml) intervention

the former two parameters raised and showed a positive correlation between them

the abnormal change in the two indexes was improved to certain extent

showing a reversing status of Th2 polarization. AM could increase the expression of T-bet mRNA and Th1 cytokines such as IFN-γ

and might reverse the Th2 predominant status in asthma patients.

关键词

AsthmaAstragalus membranaceustranscription factorT-box expressed in T cellsT helper cellsTinterferon-γ

Keywords

AsthmaAstragalus membranaceustranscription factorT-box expressed in T cellsT helper cellsTinterferon-γ

references

Holgate ST. The epidemic of allergy and asthma. Nature 1999;402(Suppl): B2–B4.

Kon OM, AB Kay. I cells and chronic asthma. Int Arch Allergy Immunol 1999;118(2–4): 133–135.

Szabo SJ, Kim ST, Costa GL, et al. A novel transcription factor, T-bet, directs Th1 lineage commitment. Cell 2000;100(6): 655–669.

Liu CT, Wang G, Luo FM, et al. Imbalanced T cell-specific transcription factors T-bet and GATA-3 contributes to type 2 T helper cell polarization in asthmatic patients. Chin J Tuberc Respir Dis 2004;27(6): 398–402.

Wang G, Liu CT, Wang ZL, et al. Antisense oligonucleotides-induced local blockade of T-bet expression leads to airway inflammation in rats. Acta Pharmacol Sin 2006;27(5): 561–567.

Finotto S, Neurath MF, Glickman JN, et al. Development of spontaneous airway changes consistent with human asthma in mice lacking T-bet. Science 2002;295(5553): 336–338

Global Initiative for Asthma (GINA). Global strategy for asthma management and prevention: NHLBI/WHO Workshop report. Bethesda: National Heart, Lung and Blood Institute. National Institute of Health, 2002.

Shen ZY, Shi SZ, Zha LG, et al. Kidney reinforcement regimen in the treatment of bronchial asthma: endocrinological and immunological studies. J Tradit Chin Med 1982;2(2): 135–140.

Li XM, Zhang TF, Sampson H, et al. The potential use of Chinese herbal medicines in treating allergic asthma. Ann Allergy Asthma Immunol 2004;93(Suppl 1): S35–S44.

Markham AW, Wilkinson JM. Complementary and alternative medicines (CAM) in the management of asthma: an examination of the evidence. J Asthma 2004;41(2): 131–139.

Yu JL, Dou DQ, Chen XH, et al. Protopanaxatriol-type ginsenosides differentially modulate type 1 and type 2 cytokines production from murine splenocytes. Planta Med 2005;71(3): 202–207.

Ko E, Park JW, Rho S, et al. Chung-Yeul-Gue-Soup-Sa-Gan-Tang, traditional Korean medicine, enhances CD4(+) T cell activates and modulates Th1/Th2 lineage development. J Pharmacol Sci 2004;94(4): 359–367.

Yesilada E, Bedir E, Calis I, et al. Effects of triterpene saponins from Astragalus speies on in vitro cytokines release. J Ethnopharmacol 2005;96(1–2): 71–77.

Wei H, Sun R, Xiao W, et al. Traditional Chinese medicine, Astragalus, reverses predominance of Th2 cytokines and their upstream transcript factors in lung cancer patients. Oncol Rep 2003;10(5): 1507–1512.

Mao SP, Cheng KL, Zhou YF. Modulatory effect of Astragalus membranaceus on Th1/Th2 cytokine in patients with herpes simplex keratitis. Chin J Integr Tradit West Med 2004;24(2): 121–123.

World Medical Association Declaration of Helsinki: ethical principles for medical research involving human subjects. JAMA 2000;284(23): 3043–3045.

Romagnani S. The role of lymphocytes in allergic disease. J Allergy Clin Immunol 2000;105(3): 399–408.

Zhang Y, Apilado R, Coleman J, et al. Interferon gamma stabilizes the T helper cell type 1 phenotype. J Exp Med 2001;194(2): 165–172.

Hardy KJ, Manger B, Newton M, et al. Molecular events involved in regulating human interferon-gamma gene expression during T cell activation. J Immunol 1987;138(7): 2353–2358.

Szabo SJ, Sullivan BM, Peng SL, et al. Molecular mechanisms regulating Th1 immune response. Annu Rev Immunol 2003;21: 713–758.

Afkarian M, Sedy JR, Yang J, et al. T-bet is a STAT1-induced regulator of IL-12R expression in naive CD4+ T cells. Natl Immunol 2002;3(6): 549–557.

Kang H, Ahn KS, Cho C, et al. Immunomodulatory effect of Astragali Radix extract on murine Th1/Th2 cell lineage development. Biol Pharm Bull 2004;27(12): 1946–1950.

Loetscher P, Uguccioni M, Bordoli L, et al. CCR5 is characteristic of Th1 lymphocytes. Nature 1998;391(6665): 344–345.

Sallusto F, Mackay CR, Lanazvecchia A. Selective expression of the eotaxin receptor CCR3 by human T helper 2 cells. Science 1997;277(5334): 2005–2007.

Ylikoski E, Kinos R, Sirkkanen N, et al. Association stuty of 15 novel single-nucleotide polymorphisms of the T-bet locus among Finnish asthma families. Clin Exp Allergy 2004; 34(7): 1049–1055.

FULL TEXT LINK

More>

Views

375

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Effect of Sanqi Oral Liquid (三芪口服液) on the expressions of CD4+, CD8+ and CD68+ cells in 5/6 nephrectomized rats with chronic renal failure

Tripterygium polyglycosid attenuates the established airway inflammation in asthmatic mice

Panax notoginseng saponins induced up-regulation, phosphorylation and binding activity of MEK, ERK, AKT, PI-3K protein kinases and GATA transcription factors in hematopoietic cells

Longer overall survival in a patient with advanced non-small cell lung cancer treated with Chinese medicine and chemotherapy

Effects and mechanisms of actions of Chinese herbal medicines for asthma

Related Author

No data

Related Institution

Guangzhou University of Chinese Medicine

Pharmaceutical Manufacturing Section, Guangdong Provincial Hospital of Traditional Chinese Medicine

Epidemiological Teaching and Research Section, Guangdong Provincial Hospital of Traditional Chinese Medicine

Animal Laboratory Center, Guangdong Provincial Hospital of Traditional Chinese Medicine

Clinical Laboratory, Guangdong Provincial Hospital of Traditional Chinese Medicine

Address：1 Caochang, xiyuan, Beijing, People's Republic of China Postal code：100091
Tel：010-62886827, 62877592 Fax：010-62874291
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05067934号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰