Effects of Ning Shen Ling Granule (宁神灵冲剂) and dehydroepiandrosterone on cognitive function in mice undergoing chronic mild stress

Zhang Xiao; Dong Yi-long; Yang Nan; Liu Yan-yong; Gao Rui-feng; Zuo  Ping-ping

doi:10.1007/s11655-007-0046-4

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Effects of Ning Shen Ling Granule (宁神灵冲剂) and dehydroepiandrosterone on cognitive function in mice undergoing chronic mild stress

Updated：2021-08-27

- Effects of Ning Shen Ling Granule (宁神灵冲剂) and dehydroepiandrosterone on cognitive function in mice undergoing chronic mild stress
- Chinese Journal of Integrative Medicine Vol. 13, Issue 1, Pages: 46-49(2007)
- Affiliations：
  
  Department of Pharmacology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Basic Research Program of China (973) (NoG2000057000)
- DOI：10.1007/s11655-007-0046-4
  CLC：
- Published：2007，
Scan for full text
Zhang, X., Dong, Yl., Yang, N. et al. Effects of Ning Shen Ling Granule (宁神灵冲剂) and dehydroepiandrosterone on cognitive function in mice undergoing chronic mild stress., Chin. J Integ. Med. 13, 46–49 (2007). https://doi.org/10.1007/s11655-007-0046-4

Zhang Xiao, Dong Yi-long, Yang Nan, et al. Effects of Ning Shen Ling Granule (宁神灵冲剂) and dehydroepiandrosterone on cognitive function in mice undergoing chronic mild stress. [J]. Chinese Journal of Integrative Medicine 13(1):46-49(2007)
Zhang, X., Dong, Yl., Yang, N. et al. Effects of Ning Shen Ling Granule (宁神灵冲剂) and dehydroepiandrosterone on cognitive function in mice undergoing chronic mild stress., Chin. J Integ. Med. 13, 46–49 (2007). https://doi.org/10.1007/s11655-007-0046-4 DOI：

Zhang Xiao, Dong Yi-long, Yang Nan, et al. Effects of Ning Shen Ling Granule (宁神灵冲剂) and dehydroepiandrosterone on cognitive function in mice undergoing chronic mild stress. [J]. Chinese Journal of Integrative Medicine 13(1):46-49(2007) DOI： 10.1007/s11655-007-0046-4.

摘要

To investigate the changes of spontaneous and cognitive behavior

and cholinergic M receptors in the brain of mice subjected to chronic mild stress (CMS)

and to determine the effect of Ning Shen Ling Granule (宁神灵冲剂

NSL) and dehydroepiandrosterone (DHEA) on them. CMS model mice were established by applying stress every day for 3 consecutive weeks with 7 kinds of unforeseeable stress sources

and they were medicated for 1 week beginning at the 3rd week of modeling. The changes in behavior were determined by Morris Water Maze and spontaneous movement test

and M-receptor binding activity in cerebral cortex

hippocampus and hypothalamus were measured by radioactive ligand assay with 3H-QNB. (1) The spontaneous movement in CMS model mice was significantly reduced

with the latency for searching platform in Morris Water Maze obviously prolonged (P<0.01)

and these abnormal changes in behavior were improved in those treated with NSL and DHEA. (2) The binding ability of M-receptor in cerebral cortex and hippocampus of CMS mice was significantly decreased as compared with those in the control group (P<0.05)

but could be restored to the normal level after intervention with NSL or DHEA. The decline of spontaneous movement and spatial learning and memory ability could be induced in animals by chronic mild stress

and that may be related to the low activity of central cholinergic M-receptors. Both NSL and DHEA could effectively alleviate the above-mentioned changes.

Abstract

To investigate the changes of spontaneous and cognitive behavior

and cholinergic M receptors in the brain of mice subjected to chronic mild stress (CMS)

and to determine the effect of Ning Shen Ling Granule (宁神灵冲剂

NSL) and dehydroepiandrosterone (DHEA) on them. CMS model mice were established by applying stress every day for 3 consecutive weeks with 7 kinds of unforeseeable stress sources

and they were medicated for 1 week beginning at the 3rd week of modeling. The changes in behavior were determined by Morris Water Maze and spontaneous movement test

and M-receptor binding activity in cerebral cortex

hippocampus and hypothalamus were measured by radioactive ligand assay with 3H-QNB. (1) The spontaneous movement in CMS model mice was significantly reduced

with the latency for searching platform in Morris Water Maze obviously prolonged (P<0.01)

and that may be related to the low activity of central cholinergic M-receptors. Both NSL and DHEA could effectively alleviate the above-mentioned changes.

关键词

chronic mild stressed modelNing Shen Ling Granuledehydroepiandrosteronecentral cholinergic M-receptorspontaneous movementMorris water maze

Keywords

chronic mild stressed modelNing Shen Ling Granuledehydroepiandrosteronecentral cholinergic M-receptorspontaneous movementMorris water maze

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