Effect of qi-tonifying and stasis-eliminating therapy (益气活血法) on expression of vascular endothelial growth factor and its receptors Flt-1, Flk-1 in the brain of intracerebral hemorrhagic rats

Zhang Zong-qi; Tang Tao; Luo Jie-kun; Huang Ju-fang; Yang Qi-dong; Li Xing-qun; Jin Yi-qiang; Qi Yong; Guo Can-jie; Zhang Hua-xian; Xing Zhi-hua; Shen Ding-zhu

doi:10.1007/s11655-007-0285-4

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Effect of qi-tonifying and stasis-eliminating therapy (益气活血法) on expression of vascular endothelial growth factor and its receptors Flt-1, Flk-1 in the brain of intracerebral hemorrhagic rats

Updated：2021-08-27

- Effect of qi-tonifying and stasis-eliminating therapy (益气活血法) on expression of vascular endothelial growth factor and its receptors Flt-1, Flk-1 in the brain of intracerebral hemorrhagic rats
- Chinese Journal of Integrative Medicine Vol. 13, Issue 4, Pages: 285-290(2007)
- Affiliations：
  
  1. Institute of the Integration of Traditional Chinese Medicine and Western Medicine, Xiangya Hospital, Central South University,Changsha,China
  2. Department of Neurology, Xiangya Hospital, Central South University,China
  3. Department of Neurobiology, Xiangya Medical College, Central South University,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30400581), Chinese Post-doctoral Science Foundation (No. 2005038224), Hunan Provincial Key Young Teachers in Universities and Hunan Provincial Funds for Key Subjects
- DOI：10.1007/s11655-007-0285-4
  CLC：
- Published：2007，
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Zhang, Zq., Tang, T., Luo, Jk. et al. Effect of qi-tonifying and stasis-eliminating therapy (益气活血法) on expression of vascular endothelial growth factor and its receptors Flt-1, Flk-1 in the brain of intracerebral hemorrhagic rats., Chin. J. Integr. Med. 13, 285–290 (2007). https://doi.org/10.1007/s11655-007-0285-4

Zhang Zong-qi, Tang Tao, Luo Jie-kun, et al. Effect of qi-tonifying and stasis-eliminating therapy (益气活血法) on expression of vascular endothelial growth factor and its receptors Flt-1, Flk-1 in the brain of intracerebral hemorrhagic rats. [J]. Chinese Journal of Integrative Medicine 13(4):285-290(2007)
Zhang, Zq., Tang, T., Luo, Jk. et al. Effect of qi-tonifying and stasis-eliminating therapy (益气活血法) on expression of vascular endothelial growth factor and its receptors Flt-1, Flk-1 in the brain of intracerebral hemorrhagic rats., Chin. J. Integr. Med. 13, 285–290 (2007). https://doi.org/10.1007/s11655-007-0285-4 DOI：

Zhang Zong-qi, Tang Tao, Luo Jie-kun, et al. Effect of qi-tonifying and stasis-eliminating therapy (益气活血法) on expression of vascular endothelial growth factor and its receptors Flt-1, Flk-1 in the brain of intracerebral hemorrhagic rats. [J]. Chinese Journal of Integrative Medicine 13(4):285-290(2007) DOI： 10.1007/s11655-007-0285-4.

摘要

To investigate the effects and mechanism of qi-tonifying and stasis-eliminating (QTSE) therapy on the expression of vascular endothelial growth factor (VEGF) and its receptors Flt-1 and Flk-1 in the brains of intracerebral hemorrhagic (model) rats. One hundred and eighty Sprague-Dawley rats were randomly divided into six groups: the normal group (n=5)

the sham-operative (SO) group (n=35)

the model group (n=35)

the QTSE group (n=35)

the QT group (n=35) and the SE group (n=35). All the rats except those in the normal group and SO group were established into an intracerebral hemorrhage(ICH) model by intracerebral injection of collagenase type VII and the latter three were orally administered with Buyang Huanwu Decoction (补阳还五汤

a classical recipe for QTSE) or with some of its components for qi-tonification and for stasis-elimination

respectively. To the other three groups

normal saline solutions were given instead. Behavioral tests were carried out in the animals randomly chosen from each group on days 1

21 and 28 after modeling. The expressions of VEGF

Flk-1 and Flt-1 were determined by immunohistochemistry and the number of vascular segments with positive expression in the injured brain area of the rats was calculated. From day 7 onwards

the asymmetric forelimb use rate in the QTSE group recovered more significantly than that in the other model groups. In the model group

the expressions of VEGF

Flk-1 and Flt-1 appeared on day 1 and reached a peak on day 21

then weakened gradually. In the QTSE group

as compared with the other model groups

a higher level of VEGF expression was shown from day 7 (P<0.01) and a higher level of Flt-1 expression was shown from the 7th day to the 21st day (P<0.01). QTSE therapy can up-regulate the expressions of VEGF and its receptors (Flk-1 and Flt-1) and improve the recovery of kinetic function in the ICH rats

which may be correlated with its action in modulating vascular regeneration to promote the reconstruction of microvascular networks in the damaged areas.

Abstract

the sham-operative (SO) group (n=35)

the model group (n=35)

the QTSE group (n=35)

a classical recipe for QTSE) or with some of its components for qi-tonification and for stasis-elimination

respectively. To the other three groups

normal saline solutions were given instead. Behavioral tests were carried out in the animals randomly chosen from each group on days 1

21 and 28 after modeling. The expressions of VEGF

Flk-1 and Flt-1 were determined by immunohistochemistry and the number of vascular segments with positive expression in the injured brain area of the rats was calculated. From day 7 onwards

the asymmetric forelimb use rate in the QTSE group recovered more significantly than that in the other model groups. In the model group

the expressions of VEGF

Flk-1 and Flt-1 appeared on day 1 and reached a peak on day 21

then weakened gradually. In the QTSE group

as compared with the other model groups

which may be correlated with its action in modulating vascular regeneration to promote the reconstruction of microvascular networks in the damaged areas.

关键词

qi-tonifying and stasis-eliminatingBuyang Huanwu Decoctionintracerebral hemorrhagevascular regenerationvascular endothelial growth factorreceptorrat

Keywords

qi-tonifying and stasis-eliminatingBuyang Huanwu Decoctionintracerebral hemorrhagevascular regenerationvascular endothelial growth factorreceptorrat

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