To explore the pharmacologic effects of Chinese medicine Bushen Huayu Jiedu Compound Recipe (补肾化瘀解毒复方

BSHYJDR) in drug-resistance cells of lung cancer. Human lung adenocarcinoma A549/DDP cell strain was selected

serum pharmacology and flow cytometer (FCM) method were adopted

S180 tumor-bearing mice and normal mice were given

through gastrogavage

different doses of a decocted concentration of BSHYJDR. Serum from the abdominal aorta was taken to observe the effect of drug-serum on cisplatin (DDP) concentration

free Ca2+ concentration and the expression of lung drug-resistance protein LRP-56 in A549/DDP cells. Compared with the drug-resistance group

the intracellular DDP concentration in the group taking a high dose and the normal group of Chinese medicine showed significant difference (P<0.05)

while no significant difference was found in the low-dose group (P>0.05). Compared with the drug-resistance group

the Ca2+ concentration in cells and the expression of LRP in lung cancer drug-resistance cells A549/DDP of the high-dose group

the low-dose group and the normal group of Chinese medicine were significantly different (all P<0.01)

the LRP expression of the normal group was obviously higher than that of the drug-resistance group (P<0.05). It was indicated that serum containing Chinese medicine BSHYJDR in the tumor-bearing mice and the normal mice had certainly different

tumor-bearing mice serum containing could improve drug concentration in lung cancer drug-resistance cells

prevent the inflow and release of Ca2+

and inhibit the expression of the drug-resistance gene in the lung cancer drug-resistance cells

which might be the mechanism of BSHYJDR in enhancing the efficacy in reversing and inhibiting tumor.